产品描述信息
NR-55472??SARS-Related Coronavirus 2, Isolate hCoV-19/USA/CA-Stanford-15_S02/2021 (Lineage B.1.617.1; Kappa Variant) in Calu-3 Cells(Viruses)|SARS-Related Coronavirus 2|Isolate hCoV-19/USA/CA-Stanford-15_S02/2021 (Lineage B.1.617.1; Kappa Variant) in Calu-3 Cells|-60°C or colder|M Suthar, B PinskyAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate hCoV-19/USA/CA-Stanford-15_S02/2021 (Lineage B.1.617.1; Kappa Variant) in Calu-3 Cells, NR-55472, contributed by Dr. Mehul Suthar and Dr. Benjamin Pinsky.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/USA/CA-Stanford-15_S02/2021 (also referred to as hCoV-19/USA/CA-SU-15_S02/2021) was isolated from a mid-turbinate nasal swab from a 29-year-old male in California, USA on March 5, 2021.
Note: Genome sequence information is provided on the Certificate of Analysis and includes an analysis of all sequence variations observed for each lot.
Under the nomenclature system introduced by GISAID (Global Initiative on Sharing All Influenza Data), SARS-CoV-2, isolate hCoV-19/USA/CA-Stanford-15_S02/2021 is assigned lineage B.1.617.1 and GISAID clade G using Phylogenetic Assignment of Named Global Outbreak LINeages (PANGOLIN) tool. SARS-CoV-2, lineage B.1.617.1 was first detected in India and labelled as a variant of interest (VOI). It was labelled as Kappa variant by the World Health Organization (WHO). The complete genome of SARS-CoV-2, isolate hCoV-19/USA/CA-Stanford-15_S02/2021 has been sequenced (GISAID: EPI_ISL_1675223). The following mutations are present in the clinical isolate: Spike D614G, Spike E154K, Spike E484Q, Spike G142D, Spike H1101D, Spike L452R, Spike P681R, Spike Q1071H, N (Nucleocapsid protein) D377Y, N R203M, NSP3 (Non-structural protein 3) S26L, NSP6 (Non-structural protein 6) I33T, NSP7a (Non-structural protein 7a) V82A, NSP3 T749I, NSP6 T77A, NSP12 (Non-structural protein 12) P323L, NSP13 (Non-structural protein 13) M429I, NSP15 (Non-structural protein 15) K259R, NSP16 (Non-structural protein 16) T93M. Note: Next-Generation Sequencing of passage four SARS-CoV-2, isolate hCoV-19/USA/CA-Stanford-15_S02/2021 at BEI Resources revealed two additional mutations as compared to the clinical isolate (GISAID: EPI_ISL_1675223): NSP14 (Non-structural protein 14) P158S and E (Envelope) F4L. Please refer to the Certificate of Analysis for more information.
Each vial contains approximately 0.1 mL of spin-clarified cell lysate and supernatant from Homo sapiens lung adenocarcinoma cells infected with SARS-CoV-2, isolate hCoV-19/USA/CA-Stanford-15_S02/2021.