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产品图片
货号/SKU
NR-55405
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55405?? Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, N440K Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, N440K Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-70°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, N440K Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55405.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), N440K variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55405 lacks the signal sequence, contains 219 residues of the SARS-CoV-2 S glycoprotein (amino acid residues R319 to K537) and features a C-terminal poly-histidine tag. NR-55405 is a variant of SARS-CoV-2 which contains the N440K mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55405 has a theoretical molecular weight of 26,500 daltons.
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The N440K mutation results in higher affinity of S to the human ACE2 receptor.
The biological activity of NR-55405 was measured by its binding ability in a functional ELISA, in which immobilized NR-55405 at 1 microgram per milliliter (100 microliters per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 10 to 78 nanograms per milliliter. The biological activity of NR-55405 was measured by its binding ability in a functional ELISA, in which immobilized Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (ACROBiosystems SAD-S35) at 1 microgram per milliliter (100 microliters per well) can bind NR-55405 with a linear range of 5 to 39 nanograms per milliliter.
Each vial contains approximately 100 micrograms of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose.
A recombinant form of the spike (S) glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), N440K variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55405 lacks the signal sequence, contains 219 residues of the SARS-CoV-2 S glycoprotein (amino acid residues R319 to K537) and features a C-terminal poly-histidine tag. NR-55405 is a variant of SARS-CoV-2 which contains the N440K mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55405 has a theoretical molecular weight of 26,500 daltons.
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The N440K mutation results in higher affinity of S to the human ACE2 receptor.
The biological activity of NR-55405 was measured by its binding ability in a functional ELISA, in which immobilized NR-55405 at 1 microgram per milliliter (100 microliters per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 10 to 78 nanograms per milliliter. The biological activity of NR-55405 was measured by its binding ability in a functional ELISA, in which immobilized Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (ACROBiosystems SAD-S35) at 1 microgram per milliliter (100 microliters per well) can bind NR-55405 with a linear range of 5 to 39 nanograms per milliliter.
Each vial contains approximately 100 micrograms of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2）的穗糖蛋白受体结合结构域（RBD）的重组形式，通过瞬态转染在人胚胎肾HEK293细胞中产生N440K变体，并通过亲和力纯化色谱法. NR-55405缺乏信号序列，含有219个残基的SARS-COV-2S糖蛋白（氨基酸残基R319至K537），并具有C末端多组氨酸标签. NR-55405是SARS-COV-2的变体，其含有与SARS-COV-2参考序列（Genpept：）. NR-55405具有26,500道尔顿的理论分子量.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.这个蛋白质
形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. N440K突变导致S对人ACE2受体的较高亲和力.
NR-55405的生物活性通过其功能性ELISA的结合能力测量，其中固定的NR-55405在每毫升/毫升（每孔100微升）中可以结合人ACE2蛋白（FC标签）（Acrobiosystems AC2-H5257） ;线性范围为每毫升10至78纳图. NR-55405的生物活性通过其功能性ELISA的结合能力测量，其中固定化的抗SARS-COV-2 RBD中和抗体，人IgG1（肌肉系统SAD-S35）在每毫升1微克（100微升） ）可以将NR-55405结合，线性范围为每毫升5至39纳图.
每个小瓶含有大约100微克纯化的重组蛋白在磷酸盐缓冲的盐水中冻干，pH7.4和10％海藻糖. sp6
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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