NR-55417来自SARS相关冠状病毒2的刺突糖蛋白S1结构域,带有C末端组氨酸标签的N501Y变体,来自HEK293细胞的重组(蛋白质）
产品图片
货号/SKU
NR-55417
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55417?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55417.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), N501Y variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55417 lacks the signal sequence, contains 670 residues of the SARS-CoV-2 S glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-55417 is a variant of SARS-CoV-2 which contains the N501Y mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55417 has a theoretical molecular weight of 76,900 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and a B.1.1.7 lineage (also known as 20B/501Y.V1, VOC202012/01 or United Kingdom variant) and a B.1.351 lineage (also known as 20C/501Y.V2 or South Africa variant) include the N501Y mutation. Structural modeling and mouse studies indicate N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence.
The biological activity of NR-55417 was measured by its binding ability in a functional ELISA, in which immobilized NR-55417 at 2 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.1 to 3 ng per mL. Immobilized NR-55417 at 2 ?g per mL (100 ?L per well) can bind Anti-SARS-CoV-2 RBD neutralizing antibody (ACROBiosystems SAD-S35); the linear range is 0.1 to 3 ng per mL. Immobilized NR-55417 at 2 ?g per mL (100 ?L per well) can bind biotinylated human ACE2/ACEH His, Avitag? protein (ACROBiosystems AC2-H82E6); the linear range is 0.1 to 3 ng per mL.
Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), N501Y variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55417 lacks the signal sequence, contains 670 residues of the SARS-CoV-2 S glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-55417 is a variant of SARS-CoV-2 which contains the N501Y mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55417 has a theoretical molecular weight of 76,900 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and a B.1.1.7 lineage (also known as 20B/501Y.V1, VOC202012/01 or United Kingdom variant) and a B.1.351 lineage (also known as 20C/501Y.V2 or South Africa variant) include the N501Y mutation. Structural modeling and mouse studies indicate N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence.
The biological activity of NR-55417 was measured by its binding ability in a functional ELISA, in which immobilized NR-55417 at 2 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.1 to 3 ng per mL. Immobilized NR-55417 at 2 ?g per mL (100 ?L per well) can bind Anti-SARS-CoV-2 RBD neutralizing antibody (ACROBiosystems SAD-S35); the linear range is 0.1 to 3 ng per mL. Immobilized NR-55417 at 2 ?g per mL (100 ?L per well) can bind biotinylated human ACE2/ACEH His, Avitag? protein (ACROBiosystems AC2-H82E6); the linear range is 0.1 to 3 ng per mL.
Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2）的穗状花序的重组形式，由人胚胎肾HEK293细胞的瞬时转染产生N501Y变体，并通过亲和层析纯化. NR-55417缺少信号序列，含有670个残基的SARS-COV-2S糖蛋白（氨基酸残基V16至R685），并具有C末端多组氨酸标签. NR-55417是SARS-COV-2的变体，其含有与SARS-COV-2参考序列（Genpept：）. NR-55417具有76,900道尔顿的理论分子量.来自SARS-COV-2的野生型糖蛋白的晶体结构已经解决了2.8埃分辨率（PDB： 6VXX ）.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S糖蛋白中的新SARS-COV-2突变目前正在研究中，以及B.1.1.7谱系（也称为
20B / 501Y.v1，VOC202012 / 01或英国变体）和B.1.351谱系（也称为20C / 501Y.V2或南非变体）包括N501Y突变.结构建模与鼠标研究
表明N501Y增加了S糖蛋白与ACE2的结合，导致SARS-COV-2毒力增加.
NR-55417的生物活性通过其功能ELISA中的结合能力测量，其中固定化的NR-55417在每毫升/ mL（每孔100μl）中的2μg（100μl）可以结合人ACE2蛋白（FC标签）（Acrobiosystems AC2-H5257） ;线性范围为每mL 0.1至3 ng.固定化NR-55417在2μg
每毫升（每孔100μL）可以结合抗SARS-COV-2 RBD中和抗体（肌肉系统SAD-S35）;线性范围为每mL 0.1至3 ng.固定的NR-55417每毫升（每孔100μl）可以结合生物素化的人ACE2 / Aceh His，Avitag TM蛋白（Acrobiosystems AC2-H82E6）;线性范围为每mL 0.1至3 ng.
每个小瓶在磷酸盐缓冲盐水，pH 7.4和10％海藻糖中含有大约100μg纯化的重组蛋白.
vipr （病毒病原体资源）提供了附加信息和工具.
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