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产品图片
货号/SKU
NR-55344
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55344?? Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells|-80°C (Note: Storage at warmer temperatures is not recommended due to a low bioburden.)|BPS BioscienceAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells, NR-55344.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the nucleocapsid (N) protein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724397) was produced by transient transfection in human embryonic kidney HEK293 cells, purified by affinity chromatography and biotinylated. NR-55344 contains the full-length SARS-CoV-2 N protein and features a C-terminal AviTag? BirA biotinylation acceptor sequence fused to a hexa-histidine tag. NR-55344 has a theoretical molecular weight of 48,000 daltons. The crystal structures for the N protein N-terminal domain and C-terminal domain from SARS-CoV-2 have been solved at 1.8 ? resolution (PDB: 7CDZ) and 1.5 ? resolution (PDB: 7CE0), respectively.
Coronavirus N proteins have an N-terminal and C-terminal domain connected by an intrinsically disordered region that has not yet been structurally solved. The N protein is the central component of virions and packages viral genomic RNA into a ribonucleoprotein complex. The SARS-CoV-2 N protein suppresses host RNAi-mediated antiviral responses through double-stranded RNA-binding activity.
Each vial contains approximately 50 ?g of purified recombinant protein in 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM dithiothreitol (DTT) and 20% glycerol.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the nucleocapsid (N) protein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724397) was produced by transient transfection in human embryonic kidney HEK293 cells, purified by affinity chromatography and biotinylated. NR-55344 contains the full-length SARS-CoV-2 N protein and features a C-terminal AviTag? BirA biotinylation acceptor sequence fused to a hexa-histidine tag. NR-55344 has a theoretical molecular weight of 48,000 daltons. The crystal structures for the N protein N-terminal domain and C-terminal domain from SARS-CoV-2 have been solved at 1.8 ? resolution (PDB: 7CDZ) and 1.5 ? resolution (PDB: 7CE0), respectively.
Coronavirus N proteins have an N-terminal and C-terminal domain connected by an intrinsically disordered region that has not yet been structurally solved. The N protein is the central component of virions and packages viral genomic RNA into a ribonucleoprotein complex. The SARS-CoV-2 N protein suppresses host RNAi-mediated antiviral responses through double-stranded RNA-binding activity.
Each vial contains approximately 50 ?g of purified recombinant protein in 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM dithiothreitol (DTT) and 20% glycerol.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关冠状病毒2（SARS-COV-2）的核衣壳（N）蛋白的重组形式，武汉-U-1（Genpept： yp_009724397 ）由瞬态产生
在人胚胎肾HEK293细胞中转染，通过亲和层析纯化和生物素化. NR-55344含有全长SARS-COV-2N蛋白，并具有融合到六征 - 组氨酸标签的C末端AVITAG?Bira Biotinylation受体序列. NR-55344具有理论分子量
48,000道尔顿.来自SARS-COV-2的N蛋白N-末端结构域和C末端结构域的晶体结构已经解决了1.8埃分辨率（PDB： 7CDZ ）和1.5?分辨率
（pdb： 7ce0 ）.
冠状病毒N蛋白质具有由尚未在结构上解决的内部无序区域连接的N末端和C末端域. N蛋白是病毒素的中心成分，并将病毒基因组RNA分成核糖核蛋白复合物. SARS-COV-2N蛋白通过双链RNA结合活性抑制宿主RNAi介导的抗病毒反应.
每个小瓶在40mM Tris-HCl pH 8.0,110mM NaCl，2.2mM KCl，3mM二硫醇（DTT）和20％甘油中，含有大约50μg的纯化的重组蛋白.
vipr （病毒病原体资源）提供了附加信息和工具.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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