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产品图片
货号/SKU
NR-55495
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55495?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Kappa Variant with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Kappa Variant with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells|-20°C or colder|BEI ResourcesAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Kappa Variant with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells, NR-55495.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Kappa variant (B.1.617.1 lineage) was produced in human embryonic kidney HEK293 cells and purified by immobilized metal affinity chromatography. NR-55495 lacks the signal sequence and contains 1196 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987; wild type numbering), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site and C-terminal octa-histidine tag fused to an AviTag? BirA biotinylation acceptor sequence. NR-55495 includes T95I, G142D, E154K, L452R, E484Q, D614G, P681R and Q1071H mutations in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55495 has a theoretical molecular weight of 139,850 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. B.1.617.1 is one of several lineages and sublineages designated Kappa by the World Health Organization (WHO) and was first identified in India. This lineage contains multiple mutations in the N-terminal domain (NTD) and the receptor-binding domain (RBD), such as L452R which has already been identified in other variants. The L452R mutation has been shown to decrease sensitivity to neutralizing antibodies, increase viral infectivity and enhance viral replication capacity.
Each vial contains approximately 100 microliters of NR-55495 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Kappa variant (B.1.617.1 lineage) was produced in human embryonic kidney HEK293 cells and purified by immobilized metal affinity chromatography. NR-55495 lacks the signal sequence and contains 1196 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987; wild type numbering), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site and C-terminal octa-histidine tag fused to an AviTag? BirA biotinylation acceptor sequence. NR-55495 includes T95I, G142D, E154K, L452R, E484Q, D614G, P681R and Q1071H mutations in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55495 has a theoretical molecular weight of 139,850 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. B.1.617.1 is one of several lineages and sublineages designated Kappa by the World Health Organization (WHO) and was first identified in India. This lineage contains multiple mutations in the N-terminal domain (NTD) and the receptor-binding domain (RBD), such as L452R which has already been identified in other variants. The L452R mutation has been shown to decrease sensitivity to neutralizing antibodies, increase viral infectivity and enhance viral replication capacity.
Each vial contains approximately 100 microliters of NR-55495 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），Kappa变体（B.1.617.1谱系）的重组形式，在人胚胎肾HEK293细胞中产生并通过固定化金属纯化亲和层析. NR-55495缺乏信号序列并含有1196个残基（Ectodomain）的SARS-COV-2S糖蛋白;通过在Furin S1 / S2切割位点（RrAr→GSAs;残基682至685）和KV→PP突变（残留物986和987;野生型编号），并包括T4 Folcon三聚化结构域，HRV3C，通过取代重组蛋白质蛋白酶切割位点和C末端Octa-组氨酸标签与Avitag?Bira Biotinylation受体序列融合.与SARS-COV-2参考序列（Genpept： qhd43416 ）. NR-55495有一个
理论分子量为139,850道尔顿. SARS-COV-2的三聚糖蛋白的晶体结构已在3.46?分辨率（PDB： 6vsb ）中得到解决.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.这个蛋白质
形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. B.1.617.1是世界卫生组织（世卫组织）指定Kappa的几个谱系和Sublineages之一，并于印度首先确定.该谱系含有N-末端域（NTD）和受体结合结构域（RBD）中的多个突变，例如已经在其他变体中识别的L452R.已经显示L452R突变降低对中和抗体的敏感性，增加病毒感染性并提高病毒复制能力.
每个小瓶在10mM Hepes，pH 7,150mM NaCl和2mM乙二胺 - 四乙酸（EDTA）中含有大约100微升NR-55495.
vipr （病毒病原体资源）提供了附加信息和工具.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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