NR-55311来自SARS相关冠状病毒2的刺突糖蛋白(稳定）,B.1.1.7谱系,带有C末端组氨酸和Avi标签,来自HEK293细胞(蛋白质）的重组
产品图片
货号/SKU
NR-55311
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55311?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, B.1.1.7 Lineage with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, B.1.1.7 Lineage with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells|-20°C or colder|BEI ResourcesAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, B.1.1.7 Lineage with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells, NR-55311.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), United Kingdom variant (UK; B.1.1.7 lineage) was produced in human embryonic kidney HEK293 cells and purified by immobilized metal affinity chromatography. NR-55311 lacks the signal sequence and contains 1193 residues (ectodomain) of the SARS-CoV-2 spike glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987; wild type numbering), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site and C-terminal octa-histidine tag fused to an AviTag? BirA biotinylation acceptor sequence. NR-55311 is derived from the B.1.1.7 lineage of SARS-CoV-2, which includes del69-70, del144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H mutations in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55311 has a theoretical molecular weight of 139,500 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2, UK variant (B.1.1.7 lineage) has been solved at 3.22 ? resolution (PDB: 7LWS).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The UK variants of SARS-CoV-2 include multiple mutations that were first identified in the United Kingdom, and the most studied is N501Y. Structural modeling and mouse studies indicate N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence.
Each vial contains approximately 100 ?L of NR-55311 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), United Kingdom variant (UK; B.1.1.7 lineage) was produced in human embryonic kidney HEK293 cells and purified by immobilized metal affinity chromatography. NR-55311 lacks the signal sequence and contains 1193 residues (ectodomain) of the SARS-CoV-2 spike glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987; wild type numbering), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site and C-terminal octa-histidine tag fused to an AviTag? BirA biotinylation acceptor sequence. NR-55311 is derived from the B.1.1.7 lineage of SARS-CoV-2, which includes del69-70, del144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H mutations in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55311 has a theoretical molecular weight of 139,500 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2, UK variant (B.1.1.7 lineage) has been solved at 3.22 ? resolution (PDB: 7LWS).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The UK variants of SARS-CoV-2 include multiple mutations that were first identified in the United Kingdom, and the most studied is N501Y. Structural modeling and mouse studies indicate N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence.
Each vial contains approximately 100 ?L of NR-55311 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），英国变异（UK; B.1.1.7谱系）的重组形式，在人胚胎肾HEK293细胞中产生并纯化通过固定化金属亲和层析. NR-55311缺乏信号序列，含有1193个残基（Ectodomain）的SARS-COV-2穗糖蛋白;通过在Furin S1 / S2切割位点（RrAr→GSAs;残基682至685）和KV→PP突变（残留物986和987;野生型编号），并包括T4 Folcon三聚化结构域，HRV3C，通过取代重组蛋白质蛋白酶切割位点和C末端Octa-组氨酸标签与Avitag?Bira Biotinylation受体序列融合. NR-55311衍生自SARS-COV-2的B.1.1.7谱系，其包括SARS-COV-2的谱系，其包括SAR69-70，DEL144，N501Y，A570D，D614G，P681H，T716i，S982A和S糖蛋白中的D1118H突变，相比之下SARS-COV-2参考序列（Genpept： qhd43416 ）. NR-55311具有139,500道尔顿的理论分子量.来自SARS-COV-2的三聚糖蛋白的晶体结构，UK变体（B.1.1.7谱系）已经解决了3.22?分辨率（PDB： 7lws ）.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. SARS-COV-2的英国变异包括在英国首次确定的多种突变，以及
大多数研究都是n501y.结构建模和小鼠研究表明N501Y增加了糖蛋白与ACE2的结合，导致SARS-COV-2毒力增加.
每个小瓶在10mM HEPES，pH 7,150mM NaCl和2mM乙二胺 - 四乙酸（EDTA）中含有约100μl的NR-55311.
vipr （病毒病原体资源）提供了附加信息和工具.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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