产品描述信息
NR-55414?? Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, K417N/E484K/N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, K417N/E484K/N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, K417N/E484K/N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55414.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), K417N/E484K/N501Y variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55414 lacks the signal sequence, contains 219 residues of the SARS-CoV-2 S glycoprotein (amino acid residues R319 to K537) and features a C-terminal poly-histidine tag. NR-55414 is a variant of SARS-CoV-2 which contains the mutations K417N, E484K and N501Y in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept:
QHD43416). NR-55414 has a theoretical molecular weight of approximately 26,600 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB:
6VXX).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and the South African variant (also known as 20C/501Y.V2 or B.1.351 lineage) includes three mutations in the RBD that may have functional significance, K417N, E484K and N501Y. Structural modeling and mouse studies indicate N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence. In addition, the E484K mutation has been identified in escape mutants for convalescent antisera.
The biological activity of NR-55414 was measured by its binding ability in a functional ELISA, in which immobilized NR-55414 at 1 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.2 to 3 ng per mL. Immobilized NR-55414 at 1 ?g per mL (100 ?L per well) can bind anti-SARS-CoV-2 RBD potent neutralizing antibody, human IgG1 (ACROBiosystems SPD-M128); the linear range is 0.1 to 0.8 ng per mL.
Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose.
Additional information and tools are available at
ViPR (Virus Pathogen Resource).
