来自 HEK293 细胞的重组(蛋白质），NR-1_Vaccinia virus

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-55420?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, P681H Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, P681H Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, P681H Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55420.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), P681H variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55420 lacks the signal sequence, contains 670 residues of the SARS-CoV-2 S glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-55420 is a variant of SARS-CoV-2 which contains the P681H mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55420 has a theoretical molecular weight of 76,900 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX). The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The P681H mutation was identified in the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01 or B.1.1.7 lineage) which emerged in the United Kingdom. P681 is part of the S1/S2 proteolytic cleavage site for furin proteases, and the mutation P681H results in increased cleavage efficiency and may alter antibody recognition sites. The biological activity of NR-55420 was measured by its binding ability in a functional ELISA, in which immobilized NR-55420 at 2 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.1 to 3 ng per mL. Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose. Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2）的穗状花序的重组形式，通过人胚胎肾HEK293细胞的瞬时转染并通过亲和色谱法纯化，产生P681H变体. NR-55420缺乏信号序列，含有670个残基的SARS-COV-2S糖蛋白（氨基酸残基V16至R685），并具有C末端多组氨酸标签. NR-55420是SARS-COV-2的变体，其含有与SARS-COV-2参考序列（Genpept：）. NR-55420具有76,900道尔顿的理论分子量.来自SARS-COV-2的野生型糖蛋白的晶体结构已经解决了2.8埃分辨率（PDB： 6VXX ）. S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜.在英国出现的SARS-COV-2变体中鉴定了P681H突变（称为20B / 501Y.V1，VOC 202012/01或B.1.1.7谱系）. P681是Furin蛋白酶S1 / S2蛋白水解切割位点的一部分，突变P681H导致裂解效率增加，并且可以改变抗体识别位点. 通过其功能ELISA的结合能力测量NR-55420的生物活性，其中固定的NR-55420在每毫升（每孔100μl）中为2μg（100μl）可以结合人ACE2蛋白（FC标签）（Acrobiosystems AC2-H5257） ;线性范围为每mL 0.1至3 ng. 每个小瓶在磷酸盐缓冲盐水，pH 7.4和10％海藻糖中含有大约100μg纯化的重组蛋白. vipr （病毒病原体资源）提供了附加信息和工具.

厂牌介绍

BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。

除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。

BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。

品牌标识

产品关键词

NR-55420 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域

带有 C 末端组氨酸标签的 P681H 变体

手机扫描二维码阅读本页

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NR-55418来自SARS相关冠状病毒2的刺突糖蛋白S1结构域,带有C末端组氨酸标签的D614G变体,来自HEK293细胞的重组(蛋白质）

NR-55418_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, D614G Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-55418_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, D614G Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)
NR-55418 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域，带有 C 末端组氨酸标签的 D614G 变体，来自 HEK293 细胞的重组（蛋白质）

货号/SKU

NR-55418

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-55418?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, D614G Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, D614G Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, D614G Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55418.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), D614G variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55418 lacks the signal sequence, contains 670 residues of the SARS-CoV-2 S glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-55418 is a variant of SARS-CoV-2 which contains the D614G mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55418 has a theoretical molecular weight of 76,800 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX). The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The D614G mutation is common to the current variants of interest and concern identified by the Centers for Disease Control and Prevention (CDC). This mutation was one of the first documented in the USA in the initial stages of the pandemic after having initially circulated in Europe. Some evidence suggests that variants with the D614G mutation are more infectious than wild-type.

The biological activity of NR-55418 was measured by its binding ability in a functional ELISA, in which immobilized NR-55418 at 2 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.2 to 3 ng per mL. Immobilized NR-55418 at 2 ?g per mL (100 ?L per well) can also bind Anti-SARS-CoV-2 neutralizing antibody (ACROBiosystems SAD-S35); the linear range is 0.2 to 3 ng per mL. The biological activity of NR-55418 was also measured by its binding ability using biosensor analysis, in which loaded ACROBiosystems AC2-H5257 can bind NR-55418; the affinity constant is 76.4 nM by ForteBio Octet Red96e. Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose. Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 通过在人胚胎肾HEK293细胞中的瞬时转染并通过亲和层析纯化，通过瞬时转染而重组形式的峰值形式的来自严重的急性呼吸综合征相关的冠状病毒2（SARS-COV-2），D614G变体，并通过亲和层析纯化. NR-55418缺少信号序列，含有670个残基的SARS-COV-2S糖蛋白（氨基酸残基V16至R685），并具有C末端多组氨酸标签. NR-55418是SARS-COV-2的变体，其含有与SARS-COV-2参考序列（Genpept：）. NR-55418具有76,800道尔顿的理论分子量.来自SARS-COV-2的野生型糖蛋白的晶体结构已经解决了2.8埃分辨率（PDB： 6VXX ）. S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. D614G突变对于当前感兴趣的变体和疾病控制中心（CDC）的中心鉴定的疑虑是常见的.这种突变是在欧洲最初循环之后在大流行之后的首次记录在美国的突变之一.有些证据表明，具有D614G突变的变体比野生型更传染. 通过其功能ELISA的结合能力测量NR-55418的生物活性，其中固定的NR-55418在每毫升（每孔100μl）中为2μg（100μl）可以结合人ACE2蛋白（FC标签）（Acrobiosystems AC2-H5257） ;线性范围为每mL 0.2至3 ng.固定化的NR-55418在2μg 每毫升（每孔100μl）也可以结合抗SARS-COV-2中和抗体（肌肉系统SAD-S35）;线性范围为每mL 0.2至3 ng. NR-55418的生物活性也是通过使用生物传感器分析来通过其结合能力测量，其中加载的辅助系统AC2-H5257可以结合NR-55418; Fortebio Octet Red96e的亲和力常数为76.4nm. 每个小瓶在磷酸盐缓冲盐水，pH 7.4和10％海藻糖中含有大约100μg纯化的重组蛋白. 附加信息和工具可在 vipr （病毒病原体资源）.

厂牌介绍

品牌标识

产品关键词

NR-55418 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域

带有 C 末端组氨酸标签的 D614G 变体

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NR-55417来自SARS相关冠状病毒2的刺突糖蛋白S1结构域,带有C末端组氨酸标签的N501Y变体,来自HEK293细胞的重组(蛋白质）

NR-55417_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-55417_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)
NR-55417 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域，带有 C 末端组氨酸标签的 N501Y 变体，来自 HEK293 细胞的重组（蛋白质）

货号/SKU

NR-55417

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-55417?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55417.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), N501Y variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55417 lacks the signal sequence, contains 670 residues of the SARS-CoV-2 S glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-55417 is a variant of SARS-CoV-2 which contains the N501Y mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55417 has a theoretical molecular weight of 76,900 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX). The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and a B.1.1.7 lineage (also known as 20B/501Y.V1, VOC202012/01 or United Kingdom variant) and a B.1.351 lineage (also known as 20C/501Y.V2 or South Africa variant) include the N501Y mutation. Structural modeling and mouse studies indicate N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence. The biological activity of NR-55417 was measured by its binding ability in a functional ELISA, in which immobilized NR-55417 at 2 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.1 to 3 ng per mL. Immobilized NR-55417 at 2 ?g per mL (100 ?L per well) can bind Anti-SARS-CoV-2 RBD neutralizing antibody (ACROBiosystems SAD-S35); the linear range is 0.1 to 3 ng per mL. Immobilized NR-55417 at 2 ?g per mL (100 ?L per well) can bind biotinylated human ACE2/ACEH His, Avitag? protein (ACROBiosystems AC2-H82E6); the linear range is 0.1 to 3 ng per mL. Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose. Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2）的穗状花序的重组形式，由人胚胎肾HEK293细胞的瞬时转染产生N501Y变体，并通过亲和层析纯化. NR-55417缺少信号序列，含有670个残基的SARS-COV-2S糖蛋白（氨基酸残基V16至R685），并具有C末端多组氨酸标签. NR-55417是SARS-COV-2的变体，其含有与SARS-COV-2参考序列（Genpept：）. NR-55417具有76,900道尔顿的理论分子量.来自SARS-COV-2的野生型糖蛋白的晶体结构已经解决了2.8埃分辨率（PDB： 6VXX ）. S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S糖蛋白中的新SARS-COV-2突变目前正在研究中，以及B.1.1.7谱系（也称为 20B / 501Y.v1，VOC202012 / 01或英国变体）和B.1.351谱系（也称为20C / 501Y.V2或南非变体）包括N501Y突变.结构建模与鼠标研究表明N501Y增加了S糖蛋白与ACE2的结合，导致SARS-COV-2毒力增加. NR-55417的生物活性通过其功能ELISA中的结合能力测量，其中固定化的NR-55417在每毫升/ mL（每孔100μl）中的2μg（100μl）可以结合人ACE2蛋白（FC标签）（Acrobiosystems AC2-H5257） ;线性范围为每mL 0.1至3 ng.固定化NR-55417在2μg 每毫升（每孔100μL）可以结合抗SARS-COV-2 RBD中和抗体（肌肉系统SAD-S35）;线性范围为每mL 0.1至3 ng.固定的NR-55417每毫升（每孔100μl）可以结合生物素化的人ACE2 / Aceh His，Avitag TM蛋白（Acrobiosystems AC2-H82E6）;线性范围为每mL 0.1至3 ng. 每个小瓶在磷酸盐缓冲盐水，pH 7.4和10％海藻糖中含有大约100μg纯化的重组蛋白. vipr （病毒病原体资源）提供了附加信息和工具.

厂牌介绍

品牌标识

产品关键词

NR-55417 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域

带有 C 末端组氨酸标签的 N501Y 变体

手机扫描二维码阅读本页

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NR-55416来自SARS相关冠状病毒2的刺突糖蛋白S1结构域,具有C末端组氨酸标签的HV69-70del变体,来自HEK293细胞的重组(蛋白质）

NR-55416_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, HV69-70del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-55416_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, HV69-70del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)
NR-55416 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域，具有 C 末端组氨酸标签的 HV69-70del 变体，来自 HEK293 细胞的重组（蛋白质）

货号/SKU

NR-55416

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-55416?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, HV69-70del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, HV69-70del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, HV69-70del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55416.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), HV69-70del variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55416 lacks the signal sequence, contains 668 residues of the SARS-CoV-2 S glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-55416 is a variant of SARS-CoV-2 which contains the HV69-70del mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55416 has a theoretical molecular weight of approximately 76,700 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX). The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and a United Kingdom variant (also known as 20B/501Y.V1, VOC202012/01 or B.1.1.7 lineage) includes the HV69-70del mutation. The biological activity of NR-55416 was measured by its binding ability in a functional ELISA, in which immobilized NR-55416 at 2 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.1 to 2 ng per mL. Immobilized NR-55416 at 2 ?g per mL (100 ?L per well) can bind anti-SARS-CoV-2 RBD neutralizing antibody, human IgG1 (ACROBiosystems SAD-S35) with a linear range of 0.1 to 2 ng per mL and biotinylated human ACE2, his, Avitag? (ACROBiosystems AC2-H82E6) with a linear range of 0.4 to 13 ng per mL. Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose. Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的穗状花序的重组形式的穗糖蛋白S1结构域冠状病毒2（SARS-COV-2），HV69-70DEL变体是通过人胚胎肾HEK293细胞的瞬时转染而产生的，并通过亲和层析纯化. NR-55416缺乏信号序列，含有668个残基的SARS-COV-2S糖蛋白（氨基酸残基V16至R685），并具有C末端多组合物标签.与SARS-COV-2参考序列（Genpept：）. NR-55416具有约76,700道尔顿的理论分子量.来自SARS-COV-2的野生型糖蛋白的晶体结构已经解决了2.8埃分辨率（PDB： 6VXX ）. S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. SARS-COV-2在S糖蛋白的突变目前正在研究中，以及英国变体（也称为20B / 501Y.V1，VOC202012 / 01或B.1.1.7谱系）包括HV69-70DEL突变. 通过其功能ELISA中的结合能力测量NR-55416的生物活性，其中固定的NR-55416在每毫升/ mL（每孔100μl）中可以结合人ACE2蛋白（FC标签）（Acrobiosystems AC2-H5257） ;线性范围为每mL 0.1至2ng.固定的NR-55416在每毫升/ mL（每孔100μl）中2μg（100μl）可以结合抗SARS-COV-2 RBD中和抗体，人IgG1（肌肉系统SAD-S35），线性范围为0.1至2ng，每mL和生物素化的人ACE2，HIS2，AVITAG?（ACROBIOSYSTEMS AC2-H82E6），线性范围为0.4至13 ng / ml. 每个小瓶在磷酸盐缓冲盐水，pH 7.4和10％海藻糖中含有大约100μg纯化的重组蛋白. vipr （病毒病原体资源）提供了附加信息和工具.

厂牌介绍

品牌标识

产品关键词

NR-55416 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域

具有 C 末端组氨酸标签的 HV69-70del 变体

手机扫描二维码阅读本页

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NR-55415来自SARS相关冠状病毒2的尖峰糖蛋白S1结构域,带有C末端组氨酸标签的Y144del变体,来自HEK293细胞的重组(蛋白质）

NR-55415_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Y144del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-55415_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Y144del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)
NR-55415 来自 SARS 相关冠状病毒 2 的尖峰糖蛋白 S1 结构域，带有 C 末端组氨酸标签的 Y144del 变体，来自 HEK293 细胞的重组（蛋白质）

货号/SKU

NR-55415

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-55415?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Y144del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Y144del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Y144del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55415.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein S1 domain from severe acute respiratory syndrome related coronavirus 2 (SARS-CoV-2), Y144del variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR 55415 lacks the signal sequence, contains 669 residues of the SARS-CoV-2 S glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-55415 is a variant of SARS-CoV-2 which contains the Y144del mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55415 has a theoretical molecular weight of approximately 76,700 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX). The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and a United Kingdom variant (also known as 20B/501Y.V1 or B.1.1.7 lineage) includes the Y144del mutation. The biological activity of NR-55415 was measured by its binding ability in a functional ELISA, in which immobilized NR-55415 at 2 ?g/mL (100 ?L/well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.1 to 3 ng/mL. Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose. Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关冠状病毒2（SARS-COV-2）的穗状花序的重组形式，通过人胚胎肾HEK293细胞的瞬时转染产生Y144DEL变体，并通过亲和层析纯化. NR 55415缺乏信号序列，含有669个残基的SARS-COV-2S糖蛋白（氨基酸残基V16至R685），并具有C末端多组合物标签. NR-55415是SARS-COV-2的变体，其含有与SARS-COV-2参考序列（Genpept： qhd43416 ）. NR-55415具有约76,700道尔顿的理论分子量.来自SARS-COV-2的野生型糖蛋白的晶体结构已经解决了2.8埃分辨率（PDB： 6vxx ）. S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. SARS-COV-2在S糖蛋白中的新SAR-COV-2突变目前正在研究中，以及英国变体（也称为20B / 501Y.V1或B.1.1.7谱系）包括Y144DEL突变. 通过其功能ELISA的结合能力测量NR-55415的生物活性，其中固定的NR-55415在2μg/ mL（100μl/孔）中可以结合人ACE2蛋白（FC标签）（Acrobiosystems AC2-H5257） ;线性范围为0.1至3 ng / ml. 每个小瓶在磷酸盐缓冲盐水，pH 7.4和10％海藻糖中含有大约100μg纯化的重组蛋白. vipr （病毒病原体资源）提供了附加信息和工具.

厂牌介绍

品牌标识

产品关键词

NR-55415 来自 SARS 相关冠状病毒 2 的尖峰糖蛋白 S1 结构域

带有 C 末端组氨酸标签的 Y144del 变体

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NR-55344来自SARS相关冠状病毒2的核衣壳蛋白,带有C末端Avi和组氨酸标签的武汉-Hu-1,来自HEK293细胞的重组(蛋白质）

NR-55344_ Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-55344_ Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells(Proteins)
NR-55344 来自 SARS 相关冠状病毒 2 的核衣壳蛋白，带有 C 末端 Avi 和组氨酸标签的武汉-Hu-1，来自 HEK293 细胞的重组（蛋白质）

货号/SKU

NR-55344

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-55344?? Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells|-80°C (Note: Storage at warmer temperatures is not recommended due to a low bioburden.)|BPS BioscienceAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells, NR-55344.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the nucleocapsid (N) protein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724397) was produced by transient transfection in human embryonic kidney HEK293 cells, purified by affinity chromatography and biotinylated. NR-55344 contains the full-length SARS-CoV-2 N protein and features a C-terminal AviTag? BirA biotinylation acceptor sequence fused to a hexa-histidine tag. NR-55344 has a theoretical molecular weight of 48,000 daltons. The crystal structures for the N protein N-terminal domain and C-terminal domain from SARS-CoV-2 have been solved at 1.8 ? resolution (PDB: 7CDZ) and 1.5 ? resolution (PDB: 7CE0), respectively. Coronavirus N proteins have an N-terminal and C-terminal domain connected by an intrinsically disordered region that has not yet been structurally solved. The N protein is the central component of virions and packages viral genomic RNA into a ribonucleoprotein complex. The SARS-CoV-2 N protein suppresses host RNAi-mediated antiviral responses through double-stranded RNA-binding activity. Each vial contains approximately 50 ?g of purified recombinant protein in 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM dithiothreitol (DTT) and 20% glycerol. Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关冠状病毒2（SARS-COV-2）的核衣壳（N）蛋白的重组形式，武汉-U-1（Genpept： yp_009724397 ）由瞬态产生在人胚胎肾HEK293细胞中转染，通过亲和层析纯化和生物素化. NR-55344含有全长SARS-COV-2N蛋白，并具有融合到六征 - 组氨酸标签的C末端AVITAG?Bira Biotinylation受体序列. NR-55344具有理论分子量 48,000道尔顿.来自SARS-COV-2的N蛋白N-末端结构域和C末端结构域的晶体结构已经解决了1.8埃分辨率（PDB： 7CDZ ）和1.5?分辨率（pdb： 7ce0 ）. 冠状病毒N蛋白质具有由尚未在结构上解决的内部无序区域连接的N末端和C末端域. N蛋白是病毒素的中心成分，并将病毒基因组RNA分成核糖核蛋白复合物. SARS-COV-2N蛋白通过双链RNA结合活性抑制宿主RNAi介导的抗病毒反应. 每个小瓶在40mM Tris-HCl pH 8.0,110mM NaCl，2.2mM KCl，3mM二硫醇（DTT）和20％甘油中，含有大约50μg的纯化的重组蛋白. vipr （病毒病原体资源）提供了附加信息和工具.

厂牌介绍

品牌标识

产品关键词

NR-55344 来自 SARS 相关冠状病毒 2 的核衣壳蛋白

带有 C 末端 Avi 和组氨酸标签的武汉-Hu-1

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NR-53798_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-53798_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)
NR-53798 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域，带有 C 末端组氨酸标签的武汉-Hu-1，来自 HEK293 细胞的重组（蛋白质）

货号/SKU

NR-53798

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-53798?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C to -80°C|Sino BiologicalAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-53798.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724390) was produced by transfection in human embryonic kidney HEK293 cells and purified. NR-53798 lacks the signal sequence, contains 670 residues of the SARS-CoV-2 spike glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-53798 has a theoretical molecular weight of 76,500 daltons. The biological activity of NR-53798 was measured by its binding ability in a functional ELISA, in which immobilized human ACE2 protein (Fc tag) (Sino Biological 10108-H05H) at 2 ?g/mL (100 ?L/well) can bind NR-53798; the half maximal effective concentration (EC₅₀) of NR-53798 is 200 to 600 ng/mL. The biological activity of NR-53798 was also measured by its binding ability using biosensor analysis, in which human ACE2 protein (Fc tag) (Sino Biological 10108-H05H) can bind NR-53798; the affinity constant is 47.7 nM. Each vial contains approximately 50 ?g of purified recombinant protein in phosphate buffered saline (PBS, pH 7.4). Note: NR-53798 was not lyophilized. Additional information and tools are available at ViPR (Virus Pathogen Resource). For more information please visit the Sino Biological website.

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept： YP_009724390 ”并纯化. NR-53798缺乏信号序列，含有670个残基的SARS-COV-2穗糖蛋白（氨基酸残基V16至R685），并具有C末端多组氨酸标签. NR-53798具有76,500道尔顿的理论分子量. NR-53798的生物活性通过其官能团中的结合能力测量，其中固定化的人ACE2蛋白（Fc标签）（SINO生物10108-H05H）在2μg/ mL（100μl/孔）中可以结合NR- 53798; NR-53798的半最大有效浓度（EC 50 ）为200至600 ng / ml. NR-53798的生物活性也通过其使用生物传感器分析的结合能力来测量，其中人ACE2蛋白（FC标签）（SINO生物10108-H05H）可以结合NR-53798;亲和力常数为47.7 nm. 每个小瓶在磷酸盐缓冲盐水中含有大约50μg的纯化的重组蛋白（PBS，pH7.4）. 注意：NR-53798没有冻干. vipr （病毒病原体资源）提供了附加信息和工具. 有关更多信息，请访问 Sino Biological网站.

厂牌介绍

品牌标识

产品关键词

NR-53798 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域

带有 C 末端组氨酸标签的武汉-Hu-1

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NR-53590来自SARS冠状病毒的刺突糖蛋白(稳定）,带有C末端组氨酸和Strep?II标签的Tor2,来自HEK293细胞的重组(蛋白质）

NR-53590_ Spike Glycoprotein (Stabilized) from SARS Coronavirus, Tor2 with C-Terminal Histidine and Strep? II Tags, Recombinant from HEK293 Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-53590_ Spike Glycoprotein (Stabilized) from SARS Coronavirus, Tor2 with C-Terminal Histidine and Strep? II Tags, Recombinant from HEK293 Cells(Proteins)
NR-53590 来自 SARS 冠状病毒的刺突糖蛋白（稳定），带有 C 末端组氨酸和 Strep? II 标签的 Tor2，来自 HEK293 细胞的重组（蛋白质）

货号/SKU

NR-53590

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌