NR-55416来自SARS相关冠状病毒2的刺突糖蛋白S1结构域,具有C末端组氨酸标签的HV69-70del变体,来自HEK293细胞的重组(蛋白质）
产品图片
货号/SKU
NR-55416
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55416?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, HV69-70del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, HV69-70del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, HV69-70del Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55416.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), HV69-70del variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55416 lacks the signal sequence, contains 668 residues of the SARS-CoV-2 S glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-55416 is a variant of SARS-CoV-2 which contains the HV69-70del mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55416 has a theoretical molecular weight of approximately 76,700 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and a United Kingdom variant (also known as 20B/501Y.V1, VOC202012/01 or B.1.1.7 lineage) includes the HV69-70del mutation.
The biological activity of NR-55416 was measured by its binding ability in a functional ELISA, in which immobilized NR-55416 at 2 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.1 to 2 ng per mL. Immobilized NR-55416 at 2 ?g per mL (100 ?L per well) can bind anti-SARS-CoV-2 RBD neutralizing antibody, human IgG1 (ACROBiosystems SAD-S35) with a linear range of 0.1 to 2 ng per mL and biotinylated human ACE2, his, Avitag? (ACROBiosystems AC2-H82E6) with a linear range of 0.4 to 13 ng per mL.
Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), HV69-70del variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55416 lacks the signal sequence, contains 668 residues of the SARS-CoV-2 S glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-55416 is a variant of SARS-CoV-2 which contains the HV69-70del mutation in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55416 has a theoretical molecular weight of approximately 76,700 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and a United Kingdom variant (also known as 20B/501Y.V1, VOC202012/01 or B.1.1.7 lineage) includes the HV69-70del mutation.
The biological activity of NR-55416 was measured by its binding ability in a functional ELISA, in which immobilized NR-55416 at 2 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.1 to 2 ng per mL. Immobilized NR-55416 at 2 ?g per mL (100 ?L per well) can bind anti-SARS-CoV-2 RBD neutralizing antibody, human IgG1 (ACROBiosystems SAD-S35) with a linear range of 0.1 to 2 ng per mL and biotinylated human ACE2, his, Avitag? (ACROBiosystems AC2-H82E6) with a linear range of 0.4 to 13 ng per mL.
Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的穗状花序的重组形式的穗糖蛋白S1结构域
冠状病毒2（SARS-COV-2），HV69-70DEL变体是通过人胚胎肾HEK293细胞的瞬时转染而产生的，并通过亲和层析纯化. NR-55416缺乏信号序列，含有668个残基的SARS-COV-2S糖蛋白（氨基酸残基V16至R685），并具有C末端多组合物标签.与SARS-COV-2参考序列（Genpept：）. NR-55416具有约76,700道尔顿的理论分子量.来自SARS-COV-2的野生型糖蛋白的晶体结构已经解决了2.8埃分辨率（PDB： 6VXX ）.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. SARS-COV-2在S糖蛋白的突变目前正在研究中，以及英国变体（也称为20B / 501Y.V1，VOC202012 / 01或B.1.1.7谱系）包括HV69-70DEL突变.
通过其功能ELISA中的结合能力测量NR-55416的生物活性，其中固定的NR-55416在每毫升/ mL（每孔100μl）中可以结合人ACE2蛋白（FC标签）（Acrobiosystems AC2-H5257） ;线性范围为每mL 0.1至2ng.固定的NR-55416在每毫升/ mL（每孔100μl）中2μg（100μl）可以结合抗SARS-COV-2 RBD中和抗体，人IgG1（肌肉系统SAD-S35），线性范围为0.1至2ng，每mL和生物素化的人ACE2，HIS2，AVITAG?（ACROBIOSYSTEMS AC2-H82E6），线性范围为0.4至13 ng / ml.
每个小瓶在磷酸盐缓冲盐水，pH 7.4和10％海藻糖中含有大约100μg纯化的重组蛋白.
vipr （病毒病原体资源）提供了附加信息和工具.
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