NR-55414来自SARS相关冠状病毒2的尖峰糖蛋白受体结合域(RBD),K417N/E484K/N501Y变体,带有C末端组氨酸标签,来自HEK293细胞(蛋白质）的重组
产品图片
货号/SKU
NR-55414
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55414?? Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, K417N/E484K/N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, K417N/E484K/N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C or colder|ACROBiosystemsAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, K417N/E484K/N501Y Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55414.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), K417N/E484K/N501Y variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55414 lacks the signal sequence, contains 219 residues of the SARS-CoV-2 S glycoprotein (amino acid residues R319 to K537) and features a C-terminal poly-histidine tag. NR-55414 is a variant of SARS-CoV-2 which contains the mutations K417N, E484K and N501Y in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55414 has a theoretical molecular weight of approximately 26,600 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and the South African variant (also known as 20C/501Y.V2 or B.1.351 lineage) includes three mutations in the RBD that may have functional significance, K417N, E484K and N501Y. Structural modeling and mouse studies indicate N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence. In addition, the E484K mutation has been identified in escape mutants for convalescent antisera.
The biological activity of NR-55414 was measured by its binding ability in a functional ELISA, in which immobilized NR-55414 at 1 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.2 to 3 ng per mL. Immobilized NR-55414 at 1 ?g per mL (100 ?L per well) can bind anti-SARS-CoV-2 RBD potent neutralizing antibody, human IgG1 (ACROBiosystems SPD-M128); the linear range is 0.1 to 0.8 ng per mL.
Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), K417N/E484K/N501Y variant was produced by transient transfection in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55414 lacks the signal sequence, contains 219 residues of the SARS-CoV-2 S glycoprotein (amino acid residues R319 to K537) and features a C-terminal poly-histidine tag. NR-55414 is a variant of SARS-CoV-2 which contains the mutations K417N, E484K and N501Y in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-55414 has a theoretical molecular weight of approximately 26,600 daltons. The crystal structure for the wild-type S glycoprotein from SARS-CoV-2 has been solved at 2.8 ? resolution (PDB: 6VXX).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and the South African variant (also known as 20C/501Y.V2 or B.1.351 lineage) includes three mutations in the RBD that may have functional significance, K417N, E484K and N501Y. Structural modeling and mouse studies indicate N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence. In addition, the E484K mutation has been identified in escape mutants for convalescent antisera.
The biological activity of NR-55414 was measured by its binding ability in a functional ELISA, in which immobilized NR-55414 at 1 ?g per mL (100 ?L per well) can bind human ACE2 protein (Fc tag) (ACROBiosystems AC2-H5257); the linear range is 0.2 to 3 ng per mL. Immobilized NR-55414 at 1 ?g per mL (100 ?L per well) can bind anti-SARS-CoV-2 RBD potent neutralizing antibody, human IgG1 (ACROBiosystems SPD-M128); the linear range is 0.1 to 0.8 ng per mL.
Each vial contains approximately 100 ?g of purified recombinant protein lyophilized in phosphate-buffered saline, pH 7.4 and 10% trehalose.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
通过在人胚胎肾HEK293细胞中的瞬时转染产生来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），K417N / E484K / N501Y变体的重组形式.通过亲和层析纯化. NR-55414缺乏信号序列，含有219个残基的SARS-COV-2 S糖蛋白（氨基酸残基R319至K537），并具有C末端多组氨酸标签. NR-55414是SARS-COV-2的变体，其含有SARS-COV-2参考序列（Genpept：）. NR-55414具有约26,600道尔顿的理论分子量.来自SARS-COV-2的野生型糖蛋白的晶体结构已经解决了2.8埃分辨率（PDB： 6VXX ）.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. SARS-COV-2在S糖蛋白中的突变目前正在研究中，南非变体（也称为20C / 501Y.V2或B.1.351谱系）包括可具有功能意义的RBD中的三个突变K417N， E484K和N501Y.结构建模和小鼠研究表明N501Y增加了糖蛋白与ACE2的结合，导致SARS-COV-2毒力增加.此外，已在恢复突变体中鉴定E484K突变，用于康复抗血清.
NR-55414的生物活性通过其官能团中的结合能力测量，其中固定的NR-55414在每毫升1μg（每孔100μl）中，可以结合人ACE2蛋白（Fc标签）（Acrobiosystems AC2-H5257） ;线性范围为每mL 0.2至3 ng.固定的NR-55414在每毫升1μg（每孔100μl）下，可以结合抗SARS-COV-2 RBD效率中和抗体，人IgG1（讽刺系统SPD-M128）;线性范围为每mL 0.1至0.8ng.
每个小瓶在磷酸盐缓冲盐水，pH 7.4和10％海藻糖中含有大约100μg纯化的重组蛋白.
vipr （病毒病原体资源）提供了附加信息和工具.
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