NR-55345具有C端Avi和组氨酸标签的人Neuropilin-1(NRP1),从HEK293细胞(蛋白质）重组而来
产品图片
货号/SKU
NR-55345
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55345?? Human Neuropilin-1 (NRP1) with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells(Proteins)||Human Neuropilin-1 (NRP1) with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells|-80°C (Note: Storage at warmer temperatures is not recommended due to a low bioburden.)|BPS BioscienceAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Human Neuropilin-1 (NRP1) with C-Terminal Avi and Histidine Tags, Recombinant from HEK293 Cells, NR-55345.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the human neuropilin-1 (NRP1) protein (GenPept: NP_001019799) was produced by transient transfection in human embryonic kidney HEK293 cells, purified by affinity chromatography and biotinylated. NR-55345 was expressed with the CD33 signal sequence, which was cleaved during secretion to yield 623 residues of the human NRP1 protein (amino acid residues F22 to K644) which features a C-terminal AviTag? BirA biotinylation acceptor sequence fused to a hexa-histidine tag. NR-55345 has a theoretical molecular weight of 75,000 daltons. The crystal structure for the human NRP1 membrane proximal c (MAM) domain has been solved at 2.24 ? resolution (PDB: 5L73).
NRP1 is a transmembrane receptor that was recently identified as a co-receptor for severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) to infect host cells. NRP1 binds furin-cleaved substrates, and the SARS-CoV-2 spike glycoprotein is cleaved by furin at the S1-S2 site. NRP1 is also highly expressed in the nasal and respiratory epithelium and enhances SARS-CoV-2 infection when co-expressed with host angiotensin converting enzyme 2 (ACE2).
Each vial contains approximately 50 ?g of purified recombinant protein in 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the human neuropilin-1 (NRP1) protein (GenPept: NP_001019799) was produced by transient transfection in human embryonic kidney HEK293 cells, purified by affinity chromatography and biotinylated. NR-55345 was expressed with the CD33 signal sequence, which was cleaved during secretion to yield 623 residues of the human NRP1 protein (amino acid residues F22 to K644) which features a C-terminal AviTag? BirA biotinylation acceptor sequence fused to a hexa-histidine tag. NR-55345 has a theoretical molecular weight of 75,000 daltons. The crystal structure for the human NRP1 membrane proximal c (MAM) domain has been solved at 2.24 ? resolution (PDB: 5L73).
NRP1 is a transmembrane receptor that was recently identified as a co-receptor for severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) to infect host cells. NRP1 binds furin-cleaved substrates, and the SARS-CoV-2 spike glycoprotein is cleaved by furin at the S1-S2 site. NRP1 is also highly expressed in the nasal and respiratory epithelium and enhances SARS-CoV-2 infection when co-expressed with host angiotensin converting enzyme 2 (ACE2).
Each vial contains approximately 50 ?g of purified recombinant protein in 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
A recombinant form of the human neuropilin-1 (NRP1) protein (GenPept: NP_001019799) was produced by transient
在人胚胎肾HEK293细胞中转染，通过亲和层析纯化和生物素化. NR-55345用CD33信号序列表示，其在分泌期间被切割，得到623个人NRP1蛋白的残基（氨基酸残基F22至K644）
特点是融合到六达组氨酸标签的C末端AVITAG?Bira Biotinylation受体序列. NR-55345具有75,000道尔顿的理论分子量.人体NRP1的晶体结构
膜近端C（MAM）域已在2.24?分辨率（PDB： 5l73 ）.
NRP1是最近被鉴定为严重的共同受体的跨膜受体
急性呼吸综合征相关的冠状病毒2（SARS-COV-2）以感染宿主细胞. NRP1结合Furin切割的底物，并且SARS-COV-2穗糖蛋白在S1-S2位点处被Furin切割. NRP1在鼻腔和呼吸上皮中也高度表达，并在与宿主血管紧张素转换酶2（ACE2）共表达时增强SARS-COV-2感染.
每个小瓶在8mM磷酸pH 7.4,110mM NaCl，2.2mM KCl和20％甘油中含有大约50μg的纯化重组蛋白.
vipr （病毒病原体资源）提供了附加信息和工具.
厂牌介绍
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