产品描述信息
NR-54000??SARS-Related Coronavirus 2, Isolate hCoV-19/England/204820464/2020(Viruses)|SARS-Related Coronavirus 2|Isolate hCoV-19/England/204820464/2020|-60°C or colder|B HallisAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate hCoV-19/England/204820464/2020, NR-54000, contributed by Bassam Hallis.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/England/204820464/2020 (also referred to as UK/VUI/3/2020 and VUI-202012/01) was isolated from a 58-year-old human male on November 24, 2020 in England, United Kingdom.
Under the nomenclature system introduced by GISAID (Global Initiative on Sharing All Influenza Data), SARS-CoV-2, isolate hCoV-19/England/204820464/2020 is assigned lineage B.1.1.7 and GISAID clade GR using Phylogenetic Assignment of Named Global Outbreak LINeages (PANGOLIN) tool. The complete genome of the clinical isolate of SARS-CoV-2, isolate hCoV-19/England/204820464/2020 has been sequenced (GISAID: EPI_ISL_683466). The following mutations are present in the clinical isolate: Spike A570D, Spike D614G, Spike D1118H, Spike H69del, Spike N501Y, Spike P681H, Spike S982A, Spike T716I, Spike V70del, Spike Y145del, N (Nucleocapsid protein) D3L, N G204R, N R203K, N S235F, NSP8 (Non-structural protein 8) Q27stop, NSP8 R52I, NSP8 Y73C, NSP3 (Non-structural protein 3) A890D, NSP3 A1305V, NSP3 I1412T, NSP3 T183I, NSP6 (Non-structural protein 6) F108del, NSP6 G107del, NSP6 S106del, NSP12 (Non-structural protein 12) P323L, NSP13 (Non-structural protein 13) K460R, NSP14 (Non-structural protein 14) E347G.
Each vial contains approximately 0.5 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells with human signaling lymphocytic activation molecule (hSLAM) infected with SARS-CoV-2, isolate hCoV-19/England/204820464/2020.