NR-53930 刚地弓形虫

NR-53930??Toxoplasma gondii, EGS SAG1-mCherry LDH2-sfGFP(Parasitic Protozoa)|Toxoplasma gondii|EGS SAG1-mCherry LDH2-sfGFP|-130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)|LM WeissAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Toxoplasma gondii, Strain EGS SAG1-mCherry LDH2-sfGFP, NR-53930.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Toxoplasma gondii (T. gondii), strain EGS SAG1-mCherry LDH2-sfGFP was deposited to BEI Resources as a transgenic strain that expresses the red fluorescent protein mCherry and superfolder green fluorescent protein (sfGFP) at specific stages of the parasite’s life cycle. Strain EGS SAG1-mCherry LDH2-sfGFP was derived from the recombinant type I/III strain EGS, isolated in 1998 from amniotic fluid of a human patient with congenital toxoplasmosis in Brazil, that is highly virulent in mice and is able to spontaneously form cysts in vitro. T. gondii, strain EGS SAG1-mCherry LDH2-sfGFP was engineered by transfection of the EGS strain with plasmids expressing mCherry and sfGFP under the control of stage-specific promoters SAG-1 (tachyzoite-specific) and LDH-2 (bradyzoite-specific), respectively. Strain EGS SAG1-mCherry LDH2-sfGFP parasites in the bradyzoite stage are positive for sfGFP and negative for mCherry. Parasites in the tachyzoite stage are positive for mCherry and negative for sfGFP. Expression of the two fluorescent proteins at distinct stages of the life cycle provides a useful tool to elucidate the mechanisms of T. gondii differentiation in vitro and in vivo. Each vial of NR-53930 contains approximately 0.5 mL of culture in cryopreservative [7.5% dimethylsulfoxide (DMSO)].