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产品图片
货号/SKU
NR-55278
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-55278?? Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Beta Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Beta Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-60°C or colder |F Krammer, F AmanatAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein Receptor Binding Domain (RBD) from SARS Related Coronavirus 2, Beta Variant with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-55278.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome related coronavirus 2 (SARS-CoV-2), Beta variant [also referred to as South Africa variant; B.1.351 lineage] was produced in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55278 lacks the signal sequence and contains 223 residues of the SARS-CoV-2 S glycoprotein RBD and features a C-terminal hexa-histidine tag. NR-55278 is a Beta variant of SARS-CoV-2, which includes K417N, E484K and N501Y mutations in the S glycoprotein RBD as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR 55278 has a theoretical molecular weight of 25,960 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2, B.1.351 lineage has been solved at 3.65 ? resolution (PDB: 7LYK).
Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. New SARS-CoV-2 mutations in the S glycoprotein are currently under study, and the Beta variant includes three mutations in the RBD that may have functional significance, K417N, E484K and N501Y. Structural modeling and mouse studies indicate N501Y increases S glycoprotein binding to ACE2, resulting in increased SARS-CoV-2 virulence. In addition, the E484K mutation has been identified in escape mutants for convalescent antisera.
Each vial contains approximately 0.1 mL of NR-55278 in phosphate buffered saline (PBS).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome related coronavirus 2 (SARS-CoV-2), Beta variant [also referred to as South Africa variant; B.1.351 lineage] was produced in human embryonic kidney HEK293 cells and purified by affinity chromatography. NR-55278 lacks the signal sequence and contains 223 residues of the SARS-CoV-2 S glycoprotein RBD and features a C-terminal hexa-histidine tag. NR-55278 is a Beta variant of SARS-CoV-2, which includes K417N, E484K and N501Y mutations in the S glycoprotein RBD as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR 55278 has a theoretical molecular weight of 25,960 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2, B.1.351 lineage has been solved at 3.65 ? resolution (PDB: 7LYK).
Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/
Each vial contains approximately 0.1 mL of NR-55278 in phosphate buffered saline (PBS).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关冠状病毒2（SARS-COV-2）的重组形式的穗糖蛋白受体结合结构域（RBD），β变体[也称为南非变体; B.1.351谱系是在人胚胎肾HEK293细胞中产生的，并通过亲和层析纯化. NR-55278缺少信号序列并含有223个残基的SARS-COV-2S糖蛋白RBD，并具有C末端Hexa-组氨酸标签. NR-55278是SARS-COV-2的β变体，与SARS-COV-2参考序列（Genpept：）. NR 55278具有25,960道尔顿的理论分子量. SARS-COV-2，B.1.351谱系的三聚糖蛋白的晶体结构已在3.65?分辨率（PDB：7LYK）下解决.
注意：有关相关项的详细协议和列表，请参阅 https://labs.icahn.msssssssssssssssm.edu/krammerlab/covid-19/ ＆nbsp;
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S糖蛋白的新SARS-COV-2突变目前正在研究中，并且β变体包括在RBD中的三个突变，其可具有功能意义，K417N，E484K和N501Y.结构建模和小鼠研究表明N501Y增加了糖蛋白与ACE2的结合，导致SARS-COV-2毒力增加.此外，已在恢复突变体中鉴定E484K突变，用于康复抗血清.
每个小瓶在磷酸盐缓冲盐水（PBS）中含有约0.1ml NR-55278.
background-color: rgb（255,255,255）;;"> vipr （病毒病原体资源）.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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