NR-54003_SARS相关冠状病毒2,分离株USA-WA1/2020,具有纳米荧光素酶基因的重组传染性克隆(icSARS-CoV-2-nLuc)(病毒）
产品图片
货号/SKU
NR-54003
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、3
产品描述信息
NR-54003??SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Recombinant Infectious Clone with Nanoluciferase Gene (icSARS-CoV-2-nLuc)(Viruses)|SARS-Related Coronavirus 2|Isolate USA-WA1/2020, Recombinant Infectious Clone with Nanoluciferase Gene (icSARS-CoV-2-nLuc)|-60°C or colder|RS BaricAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Recombinant Infectious Clone with Nanoluciferase Gene (icSARS-CoV-2-nLuc), NR-54003.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-54003 is an infectious complementary DNA (cDNA) clone of SARS-CoV-2, isolate USA-WA1/2020 with nanoluciferase (nLuc) gene, created using a reverse genetics system.
Full-length infectious cDNA of SARS-CoV-2 virus, isolate USA-WA1/2020 was generated by cloning genomic fragments separately into vector plasmids. A silent mutation (T15102A) to ablate an endogenous SacI site was created in a conserved region of the non-structural protein 12 to distinguish the infectious clone from circulating virus. A 276 base pairs region of open reading frame 7 (ORF7) in SARS-CoV-2, isolate icSARS-CoV-2-nLuc was replaced by the gene encoding nLuc. nLuc is an engineered luciferase consisting of a modified 19 kilodaltons subunit of deep sea shrimp Oplophorus gracilirostris luciferase, modified to generate brighter and stable glow-type luminescence. After assembly into full-length cDNA, full-length RNA was generated and electroporated into Cercopithecus aethiops kidney epithelial cells (Vero E6) to recover icSARS-CoV-2-nLuc virus.
Each vial contains approximately 0.3 mL of spin-clarified cell lysate and supernatant from human lung adenocarcinoma cells infected with icSARS-CoV-2-nLuc.
NR-54003 is an infectious complementary DNA (cDNA) clone of SARS-CoV-2, isolate USA-WA1/2020 with nanoluciferase (nLuc) gene, created using a reverse genetics system.
Full-length infectious cDNA of SARS-CoV-2 virus, isolate USA-WA1/2020 was generated by cloning genomic fragments separately into vector plasmids. A silent mutation (T15102A) to ablate an endogenous SacI site was created in a conserved region of the non-structural protein 12 to distinguish the infectious clone from circulating virus. A 276 base pairs region of open reading frame 7 (ORF7) in SARS-CoV-2, isolate icSARS-CoV-2-nLuc was replaced by the gene encoding nLuc. nLuc is an engineered luciferase consisting of a modified 19 kilodaltons subunit of deep sea shrimp Oplophorus gracilirostris luciferase, modified to generate brighter and stable glow-type luminescence. After assembly into full-length cDNA, full-length RNA was generated and electroporated into Cercopithecus aethiops kidney epithelial cells (Vero E6) to recover icSARS-CoV-2-nLuc virus.
Each vial contains approximately 0.3 mL of spin-clarified cell lysate and supernatant from human lung adenocarcinoma cells infected with icSARS-CoV-2-nLuc.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-54003是SARS-COV-2的感染性互补DNA（cDNA）克隆，将USA-WA1 / 2020分离为纳米琥珀酶（Nluc）基因，使用反向遗传系统产生.
SARS-COV-2病毒的全长传染cDNA，通过将基因组片段分开克隆到载体质粒中，产生分离株USA-WA1 / 2020.在非结构蛋白12的保守区域中产生沉默突变（T15102a），以将传染性克隆区分为循环病毒的保守区域产生沉默的突变（t15102a）.在SARS-COV-2中的开放阅读框7（ORF7）的276个碱基对区域，分离物ISAR-COV-2-Nluc被编码Nluc的基因取代. Nluc是一种工程化的荧光素酶，由深海虾的改性19千达氏雄序列组成，磷酸荧光素蛋白酶荧光素酶，改性为产生更亮且稳定的发光型发光.组装成全长cDNA后，产生全长RNA并将其电穿孔进入 Cercopithecus Aethiops 肾上皮细胞（Vero E6）以回收ISAR-CoV-2-Nluc病毒.
每个小瓶含有大约0.3ml旋转澄清的细胞裂解物和来自人肺腺癌细胞的上清液，感染ISSAR-COV-2-Nluc.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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