分离株 USA-WA1/2020

NR-54003??SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Recombinant Infectious Clone with Nanoluciferase Gene (icSARS-CoV-2-nLuc)(Viruses)|SARS-Related Coronavirus 2|Isolate USA-WA1/2020, Recombinant Infectious Clone with Nanoluciferase Gene (icSARS-CoV-2-nLuc)|-60°C or colder|RS BaricAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Recombinant Infectious Clone with Nanoluciferase Gene (icSARS-CoV-2-nLuc), NR-54003.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-54003 is an infectious complementary DNA (cDNA) clone of SARS-CoV-2, isolate USA-WA1/2020 with nanoluciferase (nLuc) gene, created using a reverse genetics system. Full-length infectious cDNA of SARS-CoV-2 virus, isolate USA-WA1/2020 was generated by cloning genomic fragments separately into vector plasmids. A silent mutation (T15102A) to ablate an endogenous SacI site was created in a conserved region of the non-structural protein 12 to distinguish the infectious clone from circulating virus. A 276 base pairs region of open reading frame 7 (ORF7) in SARS-CoV-2, isolate icSARS-CoV-2-nLuc was replaced by the gene encoding nLuc. nLuc is an engineered luciferase consisting of a modified 19 kilodaltons subunit of deep sea shrimp Oplophorus gracilirostris luciferase, modified to generate brighter and stable glow-type luminescence. After assembly into full-length cDNA, full-length RNA was generated and electroporated into Cercopithecus aethiops kidney epithelial cells (Vero E6) to recover icSARS-CoV-2-nLuc virus. Each vial contains approximately 0.3 mL of spin-clarified cell lysate and supernatant from human lung adenocarcinoma cells infected with icSARS-CoV-2-nLuc.

NR-54002??SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Recombinant Infectious Clone with Enhanced Green Fluorescent Protein (icSARS-CoV-2-eGFP)(Viruses)|SARS-Related Coronavirus 2|Isolate USA-WA1/2020, Recombinant Infectious Clone with Enhanced Green Fluorescent Protein (icSARS-CoV-2-eGFP)|-60°C or colder|RS BaricAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Recombinant Infectious Clone with Enhanced Green Fluorescent Protein (icSARS-CoV-2-eGFP), NR-54002.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-54002 is an infectious complementary DNA (cDNA) clone of SARS-CoV-2, isolate USA-WA1/2020 with enhanced green fluorescent protein (eGFP), created using a reverse genetics system. Note: Genome sequence information is provided on the Certificate of Analysis and includes an analysis of all sequence variations observed for each lot. Full-length infectious cDNA of SARS-CoV-2 virus, isolate USA-WA1/2020 was generated by cloning seven genomic fragments separately into vector plasmids. A silent mutation (T15102A) to ablate an endogenous SacI site was created in a conserved region of the non-structural protein 12 to distinguish the infectious clone from circulating virus. A 276 base pairs region of open reading frame 7 (ORF7) in SARS-CoV-2, isolate icSARS-CoV-2-GFP was replaced by an approximately 720 base pair eGFP. eGFP is a modified version of the GFP gene designed for brighter fluorescence, in which the codon utilization has been maximized for translation in eukaryotic cells. After assembly into full-length cDNA, full-length RNA was generated and electroporated into Cercopithecus aethiops kidney epithelial cells (Vero E6) to recover icSARS-CoV-2-eGFP virus. Replication of the recombinant virus icSARS-CoV-2-eGFP was confirmed, and the virus could be successfully passaged serially in cell culture to titers equivalent to the clinical isolate. The complete genome of recombinant virus icSARS-CoV-2-eGFP has been sequenced (GenBank: MT461670). Each vial contains approximately 0.3 mL of spin-clarified cell lysate and supernatant from human lung adenocarcinoma cells infected with icSARS-CoV-2-eGFP.

NR-54001??SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Recombinant Infectious Clone (icSARS-CoV-2-WT)(Viruses)|SARS-Related Coronavirus 2|Isolate USA-WA1/2020, Recombinant Infectious Clone (icSARS-CoV-2-WT)|-60°C or colder|RS BaricAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Recombinant Infectious Clone (icSARS-CoV-2-WT), NR-54001.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Note: NR-54001 label lists this clone as wildtype (WT); however, NR-54001 is not WT and includes a T15102A silent mutation. NR-54001 is an infectious complementary DNA (cDNA) clone of SARS-CoV-2, isolate USA-WA1/2020 created using a reverse genetics system. Note: Genome sequence information is provided on the Certificate of Analysis and includes an analysis of all sequence variations observed for each lot. Full-length infectious cDNA of SARS-CoV-2 virus, isolate USA-WA1/2020 was generated by cloning seven genomic fragments separately into vector plasmids. A silent mutation (T15102A) to ablate an endogenous SacI site was created in a conserved region of the non-structural protein 12 to distinguish the infectious clone from circulating virus. After assembly into full-length cDNA, full-length RNA was generated and electroporated into Cercopithecus aethiops kidney epithelial cells (Vero E6) to recover icSARS-CoV-2-WT virus. Replication of the recombinant virus icSARS-CoV-2-WT was confirmed, and the virus could be successfully passaged serially in cell culture to titers equivalent to the clinical isolate. The complete genome of recombinant virus icSARS-CoV-2-WT has been sequenced (GenBank: MT461669). Each vial contains approximately 0.3 mL of spin-clarified cell lysate and supernatant from Homo sapiens lung adenocarcinoma cells infected with icSARS-CoV-2-WT.