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产品图片
货号/SKU
NR-55293
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-55293?? Homo sapiens Embryonic Kidney Epithelial Cells Expressing Transmembrane Protease, Serine 2 and Human Angiotensin-Converting Enzyme 2 [293T-ACE2.TMPRSS2 (mCherry)](Cell Banks)|Homo sapiens|Homo sapiens Embryonic Kidney Epithelial Cells Expressing Transmembrane Protease, Serine 2 and Human Angiotensin-Converting Enzyme 2 [293T-ACE2.TMPRSS2 (mCherry)]|-100°C or colder, preferably in the vapor phase of a liquid
nitrogen freezer|C WeissAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Homo sapiens Embryonic Kidney Epithelial Cells Expressing Transmembrane Protease, Serine 2 and Human Angiotensin-Converting Enzyme 2 [293T-ACE2.TMPRSS2 (mCherry)], NR-55293."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-55293 [293T-ACE2.TMPRSS2 (mCherry)] contains a preparation of Homo sapiens embryonic kidney epithelial cells (HEK-293T) which were modified to express the transmembrane protease, serine 2 (TMPRSS2) and angiotensin-converting enzyme 2 (ACE2). ACE2 is a human receptor that is expressed widely, including in heart, kidney, small intestine and lung cells, and is involved in the regulation of hypertension and other cardiovascular diseases. The SARS-Related Coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral binding to the host ACE2 receptor. TMPRSS2 has been shown to be involved in proteolytically activating SARS-CoV. Cells expressing human TMPRSS2 were found to be highly susceptible to infection by SARS-CoV-2.
293T-ACE2.TMPRSS2 (mCherry) stable cell line was created by transducing 293T-ACE2 cells (BEI Resources NR-52511) with lentivirus encoding TMPRSS2 and mCherry as a bicistronic transcript. The bulk transduced population was single-cell sorted by flow cytometry based on mCherry positivity and the clone displaying intermediate levels of mCherry expression and maximum pseudovirus infectivity titer was expanded as 293T-ACE2.TMPRSS2 (mCherry) cells.
Each vial contains approximately 1 mL of cell culture suspension frozen in Dulbecco’s Modified Eagle’s Medium containing 10% heat-inactivated fetal bovine serum and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
NR-55293 [293T-ACE2.TMPRSS2 (mCherry)] contains a preparation of Homo sapiens embryonic kidney epithelial cells (HEK-293T) which were modified to express the transmembrane protease, serine 2 (TMPRSS2) and angiotensin-converting enzyme 2 (ACE2). ACE2 is a human receptor that is expressed widely, including in heart, kidney, small intestine and lung cells, and is involved in the regulation of hypertension and other cardiovascular diseases. The SARS-Related Coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral binding to the host ACE2 receptor. TMPRSS2 has been shown to be involved in proteolytically activating SARS-CoV. Cells expressing human TMPRSS2 were found to be highly susceptible to infection by SARS-CoV-2.
293T-ACE2.TMPRSS2 (mCherry) stable cell line was created by transducing 293T-ACE2 cells (BEI Resources NR-52511) with lentivirus encoding TMPRSS2 and mCherry as a bicistronic transcript. The bulk transduced population was single-cell sorted by flow cytometry based on mCherry positivity and the clone displaying intermediate levels of mCherry expression and maximum pseudovirus infectivity titer was expanded as 293T-ACE2.TMPRSS2 (mCherry) cells.
Each vial contains approximately 1 mL of cell culture suspension frozen in Dulbecco’s Modified Eagle’s Medium containing 10% heat-inactivated fetal bovine serum and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-55293 [293T-ACE2.TMPRSS2（MCHERRY）]含有胚胎肾上上皮细胞（HEK-293T）的制备，其被修饰以表达跨膜蛋白酶，丝氨酸2（TMPRSS2）和血管紧张素转换酶2（ACE2）. ACE2是一种人类受体，广泛表达，包括心脏，肾，小肠和肺细胞，并参与高血压和其他心血管疾病的调节. SARS相关的冠状病毒2（SARS-COV-2）穗糖蛋白介导病毒结合到宿主ACE2受体.已经显示TMPRSS2参与蛋白水解激活SARS-COV.发现表达人TMPRSS2的细胞高易受SARS-COV-2感染的细胞.
293T-ACE2.TMPRS2（MCHERRY）稳定的细胞系通过将293T-ACE2细胞（BEI资源NR-52511）与编码TMPRSS2和MCHERRY的慢病毒作为双顺声转录物进行创建.块状转导群是通过基于MCHERRY阳性的流式细胞术分类的单细胞，并且克隆显示中间水平的MCHERRY表达和最大假瘤病毒滴度滴定为293T-ACE2.TMPRS2（MCHERRY）细胞.
每个小瓶在Dulbecco改性鹰培养基中含有约1ml细胞培养悬浮液，其含有10％热灭活的胎儿牛血清和DMSO（10％）冷冻保存.提供足够的细胞以引发至少一种新培养物.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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