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产品图片
货号/SKU
NR-53931
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-53931??Toxoplasma gondii, EGS SAG1-mCherry LDH2-sfGFP MSF-BFP(Parasitic Protozoa)|Toxoplasma gondii|EGS SAG1-mCherry LDH2-sfGFP MSF-BFP|-130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)|LM WeissAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Toxoplasma gondii, Strain EGS SAG1-mCherry LDH2-sfGFP MSF-BFP, NR-53931.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Toxoplasma gondii (T. gondii), strain EGS SAG1-mCherry LDH2-sfGFP MSF-BFP was deposited to BEI Resources as a transgenic strain that expresses the red fluorescent protein mCherry, superfolder green fluorescent protein (sfGFP) and blue fluorescent protein (BFP) at specific stages of the parasite’s life cycle. Strain EGS SAG1-mCherry LDH2-sfGFP MSF-BFP was derived from the recombinant type I/III strain EGS, isolated in 1998 from amniotic fluid of a human patient with congenital toxoplasmosis in Brazil, that is highly virulent in mice and is able to spontaneously form cysts in vitro.
T. gondii, strain EGS SAG1-mCherry LDH2-sfGFP MSF-BFP was engineered by transfection of the EGS strain with plasmids expressing mCherry, sfGFP and BFP under the control of stage-specific promoters SAG-1 (tachyzoite-specific), LDH-2 (bradyzoite-specific) and MSF (merozoite-specific), respectively. Strain EGS SAG1-mCherry LDH2-sfGFP MSF-BFP parasites in the bradyzoite stage are positive for sfGFP and negative for mCherry and BFP. Parasites in the tachyzoite stage are positive for mCherry and negative for sfGFP and BFP. Merozoite-stage parasites are positive for BFP and negative for sfGFP and mCherry. Expression of the three fluorescent proteins at distinct stages of the life cycle provides a useful tool to elucidate the mechanisms of T. gondii differentiation in vitro and in vivo.
Each vial of NR-53931 contains approximately 0.5 mL of culture in cryopreservative [7.5% dimethylsulfoxide (DMSO)].
Toxoplasma gondii (T. gondii), strain EGS SAG1-mCherry LDH2-sfGFP MSF-BFP was deposited to BEI Resources as a transgenic strain that expresses the red fluorescent protein mCherry, superfolder green fluorescent protein (sfGFP) and blue fluorescent protein (BFP) at specific stages of the parasite’s life cycle. Strain EGS SAG1-mCherry LDH2-sfGFP MSF-BFP was derived from the recombinant type I/III strain EGS, isolated in 1998 from amniotic fluid of a human patient with congenital toxoplasmosis in Brazil, that is highly virulent in mice and is able to spontaneously form cysts in vitro.
T. gondii, strain EGS SAG1-mCherry LDH2-sfGFP MSF-BFP was engineered by transfection of the EGS strain with plasmids expressing mCherry, sfGFP and BFP under the control of stage-specific promoters SAG-1 (tachyzoite-specific), LDH-2 (bradyzoite-specific) and MSF (merozoite-specific), respectively. Strain EGS SAG1-mCherry LDH2-sfGFP MSF-BFP parasites in the bradyzoite stage are positive for sfGFP and negative for mCherry and BFP. Parasites in the tachyzoite stage are positive for mCherry and negative for sfGFP and BFP. Merozoite-stage parasites are positive for BFP and negative for sfGFP and mCherry. Expression of the three fluorescent proteins at distinct stages of the life cycle provides a useful tool to elucidate the mechanisms of T. gondii differentiation in vitro and in vivo.
Each vial of NR-53931 contains approximately 0.5 mL of culture in cryopreservative [7.5% dimethylsulfoxide (DMSO)].
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
毒素弓形虫（T.Gondii），菌株Egs SAG1-MCHERRYLDH2-SFGFP MSF-BFP作为表达红色荧光蛋白MCHERRY的转基因菌株，超级粘合剂
绿色荧光蛋白（SFGFP）和蓝色荧光蛋白（BFP）在寄生虫生命周期的特定阶段.菌株Egs SAG1-MCHERRY LDH2-SFGFP MSF-BFP衍生自重组型I / III菌株EGS，1998年从巴西中先天性毒素的人类患者的羊水中分离出来，在小鼠中是高度毒性的，并且能够自发地自发形成囊肿在体外.
t. Gondii ，通过在阶段特异性启动子SAG-1（特异性特异性特异性）的控制下，通过用表达MCHERRY，SFGFP和BFP的质粒转染EGS菌株来设计菌株EGS SAG1-MCHERRY LDH2-SFGFP MSF-BFP. LDH-2（特异性Bradyzoite特异性）和MSF（Merozoite特异性）.在Bradyzoite阶段中的菌株EGS SAG1-MCHERRY LDH2-SFGFP MSF-BFP寄生液对于SFGFP和MCHERRY和BFP的阴性是阳性的.寄生虫中的寄生虫阶段为麦克里氏菌和SFGFP和BFP的阴性. Merozoite-阶段寄生虫对BFP和SFGFP和MCHERRY的阴性是阳性的.三种荧光蛋白在生命周期的不同阶段的表达提供了一种有用的工具，以阐明 T的机制. Contii 分化在体外（体外）在体内.
NR-53931的每个小瓶中含有约0.5ml培养物在冷冻押渣中[7.5％二甲基磺砜（DMSO）].
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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