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产品图片

货号/SKU
NR-53821
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-53821??Homo sapiens, Human Lung Carcinoma Cells (A549) Expressing Human Angiotensin-Converting Enzyme 2(Cell Banks)|Homo sapiens|Human Lung Carcinoma Cells (A549) Expressing Human Angiotensin-Converting Enzyme 2|-100°C or colder, preferably in the vapor phase of a liquid
nitrogen freezer|R BaricAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Human Lung Carcinoma Cells (A549) Expressing Human Angiotensin-Converting Enzyme 2, NR-53821."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
NR-53821 contains a preparation of human (Homo sapiens) lung carcinoma epithelial cells (A549) expressing human angiotensin-converting enzyme 2 (ACE2). A549-hACE2 cells were created by stable insertion of human ACE2 driven by elongation factor-1a (EF-1a) promoter using the Sleeping Beauty transposon system pSBbi-BB (Addgene 60521). pSBbi-BB contains a blasticidin resistance gene for selection of transfected cells.
ACE2 is a human receptor that is expressed widely, including in heart, kidney, small intestine and lung cells, and is involved in the regulation of hypertension and other cardiovascular diseases. The SARS-Related Coronavirus 2 spike glycoprotein mediates viral binding to the host ACE2 receptor. This protein forms a trimer, and when bound to ACE2, allows fusion of the viral and cellular membranes, allowing viral entry and replication.
Each vial contains approximately 1 mL of cell culture suspension frozen in Dulbecco’s Modified Eagle’s Medium (69%) containing MEM non-essential amino acid solution (1%), fetal bovine serum (20%) and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
NR-53821 contains a preparation of human (Homo sapiens) lung carcinoma epithelial cells (A549) expressing human angiotensin-converting enzyme 2 (ACE2). A549-hACE2 cells were created by stable insertion of human ACE2 driven by elongation factor-1a (EF-1a) promoter using the Sleeping Beauty transposon system pSBbi-BB (Addgene 60521). pSBbi-BB contains a blasticidin resistance gene for selection of transfected cells.
ACE2 is a human receptor that is expressed widely, including in heart, kidney, small intestine and lung cells, and is involved in the regulation of hypertension and other cardiovascular diseases. The SARS-Related Coronavirus 2 spike glycoprotein mediates viral binding to the host ACE2 receptor. This protein forms a trimer, and when bound to ACE2, allows fusion of the viral and cellular membranes, allowing viral entry and replication.
Each vial contains approximately 1 mL of cell culture suspension frozen in Dulbecco’s Modified Eagle’s Medium (69%) containing MEM non-essential amino acid solution (1%), fetal bovine serum (20%) and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
此项目目前处于我们的生产队列中.请允许充足的时间进行分销批次.
NR-53821含有人类的制备,表达人血管紧张素转化酶2(ACE2)的人类(Homo sapiens)肺癌上皮细胞(A549).通过使用睡眠美容转座子系统PSBBI-BB(Addgene 60521)稳定地插入由延长因子-1a(EF-1A)启动子驱动的人ACE2,通过稳定插入1549-HACE2细胞. psbbi-bb含有用于选择转染细胞的痉挛性抗性基因.
ACE2是一种人类受体,广泛表达,包括心脏,肾,小肠和肺细胞,并参与高血压和其他心血管疾病的调节. SARS相关的冠状病毒2穗糖蛋白介导病毒结合到宿主ACE2受体.该蛋白质形成三聚体,并且当与ACE2结合时,允许融合病毒和细胞膜,允许病毒进入和复制.
每个小瓶含有大约1ml的含有MEM非必需氨基酸溶液(1%),胎儿牛血清(20%)和DMSO(10%)冷冻保存的含有MEM的改性鹰培养基(69%)的细胞培养悬浮液.提供足够的细胞以引发至少一种新培养物.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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