产品描述信息
MRA-809?? Plasmid pMPMII, for Transfection in Plasmodium falciparum(Plasmid/Vectors)|Plasmodium falciparum|Plasmid pMPMII, for Transfection in Plasmodium falciparum|-80°C or colder|DE GoldbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pMPMII, for Transfection in Plasmodium falciparum, MRA-809, contributed by Daniel E. Goldberg."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. MRA-809 is an Escherichia coli (E. coli) expression plasmid, pMPMII, encoding the plasmepsin II gene from Plasmodium falciparum, strain HB3. It encodes mature plasmepsin II with an N-terminal MGSSN sequence. The parent vector is pET-3d (Novagen) and the recommmended host for expression is E. coli BL21 (DE3) RIL (requires chloramphenicol). NcoI and BamHI flank the plasmepsin II ORF (5' and 3', respectively). Ampicillin (E. coli) was incorporated as a selectable marker. The resulting size of the plasmid is 4.64 kilobases.Each vial of MRA-809 contains approximately 20 ?L of plasmid DNA in TE buffer at a concentration of 10 ng/?L. Luker, K. E., et al. "Kinetic Analysis of Plasmepsins I and II, Aspartic Proteases of the Plasmodium falciparum Digestive Vacuole." Mol. Biochem. Parasitol. 79 (1996): 71-78. PubMed: 8844673.Istvan, E. S. and D. E. Goldberg. "Distal Substrate Interactions Enhance Plasmepsin Activity." J. Biol. Chem. 280 (2005): 6890-6896. PubMed: 15574427.