产品描述信息
MRA-98?? Plasmid pSOCS2, for Transfection in Plasmodium falciparum(Plasmid/Vectors)|Plasmodium falciparum|Plasmid pSOCS2, for Transfection in Plasmodium falciparum|4°C|Y Wu, TE WellemsAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pSOCS2, for Transfection in Plasmodium falciparum, MRA-98, contributed by Yimin Wu and Thomas E. Wellems."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. MRA-98 is a Plasmodium falciparum (P. falciparum) transformation plasmid, pSOCS2, which incorporates gene control elements of hsp86. To construct pSOCS2, plasmid pHRPSCAT was restricted with KpnI and NsiI, thereby removing the 5'-hrp3 sequence. The linearized plasmid was then religated with an adaptor pair, 5'-CGAGATTCGAATAAAATGCA-3' and 5'-TTTTATTCGAATCTCGGTAC-3', that introduced the BstBI site and 10 bp of the 5'-hsp86 sequence upstream of s-cat start codon. The full 5'-hsp86 flanking sequence was then amplified from Dd2 genomic DNA with primers 5'-TAAATGGTACCTTGATATATTTTTAGATATATGGAT-3' and 5'-TCCGTATGCATTTTATTCGAAATGTG-3', restricted with KpnI and BstBI, and inserted between the KpnI and BstBI sites of this plasmid. In the final step, the 3'hsp86 sequence was amplified from Dd2 genomic DNA with primers 5'-GACTAAAGCTTTTATATAATATATTTATGTACTC-3' and 5'-CATAATGCTGCAGTATTTGATGAATTAACTACAC-3', restricted with HindIII and PstI, and inserted between the HindIII and PstI of the plasmid to yield pSOCS2. Plasmid pSOCS2 was designed for transfection into the genome of P. falciparum parasites. The resulting size of the plasmid is 6.8 kilobases. The sequence has not been confirmed by BEI Resources.