产品描述信息
MRA-844?? Plasmid pCG6-attB, for Integration in Plasmodium falciparum(Plasmid/Vectors)|Plasmodium falciparum|Plasmid pCG6-attB, for Integration in Plasmodium falciparum|-20°C or colder|DA FidockAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pCG6-attB, for Integration in Plasmodium falciparum, MRA-844, contributed by David A. Fidock."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. MRA-844 is a targeting plasmid, pCG6-attB, for attB site integration into the cg6 locus in Plasmodium falciparum to create an integrase-permissive host for recombination. This single cross-over plasmid, pCG6-attB, contains an attB sequence from Mycobacterium smegmatis for insertion into the disrupted locus and human dihydrofolate reductase (DHFR) for selection with WR99210. The resulting size of the plasmid is 6.431 kilobases (GenBank: DQ813651). Dual transfection attB and Bxb1 integrase encoding plasmids are available (BEI Resources MRA-846 and MRA-847) for single-copy plasmid integration into an attB+ host.Each vial of MRA-844 contains approximately 500 ng of plasmid DNA in TE buffer at a concentration of 10 ng/?L. Nkumrah, L. J., et al. "Efficient Site-Specific Integration in Plasmodium falciparum Chromosomes Mediated by Mycobacteriophage Bxb1 Integrase." Nat. Methods 3 (2006): 615-621. PubMed: 16862136.