产品描述信息
MRA-662?? Hybridoma b5 Anti-Plasmodium berghei Phosphoprotein, Homologue to HSP70 Co-Chaperone (HIP, p48)(Cell Banks)|Plasmodium berghei|Hybridoma b5 Anti-Plasmodium berghei Phosphoprotein, Homologue to HSP70 Co-Chaperone (HIP, p48)|-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer|MF WiserAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma b5 Anti-Plasmodium berghei Phosphoprotein, Homologue to HSP70 Co-Chaperone (HIP, p48), MRA-662, contributed by Mark F. Wiser."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. The murine hybridoma cell line, b5, was generated by the fusion of mouse myeloma cells (P3X63.Ag8.6.5.3) with splenocytes from mice immunized with purified 58 kDa phosphoprotein from Plasmodium berghei. This reagent was authenticated by the contributor and tested for mycoplasma.Each vial of MRA-662 contains approximately 0.5 mL of hybridoma cells (5 × 106 cells/mL) in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO).Hybridoma b5 produces monoclonal antibody (isotype IgG1) that specifically recognizes a 58 kDa phosphoprotein identified as a Plasmodium homolog to the heat shock protein 70 (HSP70) co-chaperone (known either as HIP or p48), and reacts with the protein from all rodent Plasmodium species.Wiser, M. F. and B. Plitt. "Plasmodium berghei, P. chabaudi, and P. falciparum: Similarities in Phosphoproteins and Protein Kinase Activities and Their Stage Specific Expression." Exp. Parasitol. 64 (1987): 328-335. PubMed: 3315732.Wiser, M. F., et al. "Further Characterization of a 58 kDa Plasmodium berghei Phosphoprotein as a Cochaperone." Mol. Biochem. Parasitol. 83 (1996): 25-33. PubMed: 9010839.