产品描述信息
MRA-1053?? Plasmodium falciparum, piggyBac Clone 0703292202G02, PB-93 (Parasitic Protozoa)|Plasmodium falciparum|Plasmodium falciparum, piggyBac Clone 0703292202G02, PB-93 |Liquid nitrogen vapor phase|JH AdamsAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, piggyBac Clone 0703292202G02, PB-93, MRA-1053, contributed by John H. Adams."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NOTE: piggyBac clones supplied from the MR4 collection have not been resequenced following growth. Recipients of piggyBac clones should independently validate the insertion site reported by the depositor's laboratory, and it is recommended by the depositor to reclone the parasite. See the piggyBac clone spreadsheet for details of this clone including insertion site and insertion sequence.Plasmodium falciparum, piggyBac clone 0703292202G02, PB-93, is a genetic recombinant constructed by integration of the piggyBac insertion element 274 base pairs upstream of PF08_0018 and 669 base pairs downstream of MAL8P1.19. The transfected plasmid is pLBacII-HDGH, and the invasion rate is 59.5% of wildtype. The insertion locus is: TTAAAATATTTTTGATTTATATCTTTTCCAGTTTGTATGTCAAAAATTTTATGTGATA CAATTAATTTTATATATTAAGCAATAAAGAAAAATATGTGATATAATAAAACAATTGTGTTTTCACACATAAA TATATTCCTCACTATATATATATATATATATATATATATATATATATATATATATTTTTTTTTTTTTTTATTT TGTTTTACTGAAAAGAGCATAGGGGCTGTATAATATAAAATAATAAATGTTTAGATAACCATGTGGACTTAAT ATGAACAAGATAAAAATAAGAAAATTTGAGGATCC ACATGTTTGGATATATGAAAATGTATTATTAAATAAAA CATTGAAATTAAAAGAAATCGAAAAAAAGAAAGATAATAAAAATGTTCCAAATGCATTATTGCATGAAGAAGC TTGTAAGAAATTAAAAGGCATATTAAAAGAATGTAAAAAATATGACAAAGAATTACAAGAAATTAAGAATAAA AAAGAATCMRA-1053 is supplied as a cryopreserved stock from mixed blood stage in vitro culture.Balu, B., et al. "High-Efficiency Transformation of Plasmodium falciparum by the Lepidopteran Transposable Element piggyBac." Proc. Natl. Acad. Sci. USA 102 (2005): 16391-16396. PubMed: 16260745.Balu, B., et al. "piggyBac is an Effective Tool for Functional Analysis of the Plasmodium falciparum Genome." BMC Microbiol. 9 (2009): 83. PubMed: 19422698.Balu, B., et al. "A Genetic Screen for Attenuated Growth Identifies Genes Crucial for Intraerythrocytic Development of Plasmodium falciparum." PLoS One 5 (2010): e13282. PubMed: 20949012.