产品描述信息
NR-2649?? Genomic DNA from Escherichia coli, Strain RIMD 0509952 (Nucleic Acids)|Escherichia coli|Genomic DNA from Escherichia coli, Strain RIMD 0509952 |-20°C|T Honda, M YohAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Escherichia coli, Strain RIMD 0509952, NR-2649."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Genomic DNA was isolated from a preparation of Escherichia coli, strain RIMD 0509952, serotype O157:H7. The bacterial preparation was produced by propagation of BEI Resources NR-12.The enterohemorrhagic E. coli (EHEC) strain RIMD 0509952 was isolated from a patient during an outbreak of hemorrhagic colitis and hemolytic uremic syndrome in Sakai City, Osaka Prefecture, Japan. E. coli RIMD 0509952 and many other EHEC strains encode potent toxins, similar to those of Shigella dysenteriae, which can cause severe intestinal, kidney and central nervous system disease. E. coli RIMD 0509952 carries two plasmid species: 1) pO157 (92,721 bp) and 2) pOSAK1 (3,306 bp). Virulence-associated genes are located on both the chromosome and pO157.The complete sequence of the chromosome (5,498,450 bp; GenBank: NC_002695), pO157 (GenBank: NC_002128) and pOSAK1 (GenBank: NC_002127) from E. coli strain RIMD 0509952 have been determined.NR-2649 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene as well as three virulence markers on the chromosome. The presence of plasmid pO157 has been confirmed by PCR amplification of a approximately 3200 bp sequence.Each vial contains 4-6 micrograms of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH approximately 7.4). The vial should be centrifuged prior to opening.