Western Reserve(NIAID

NR-2639?? Genomic DNA from Vaccinia Virus, Western Reserve (NIAID, Tissue Culture Adapted) (Nucleic Acids)|Vaccinia virus|Genomic DNA from Vaccinia Virus, Western Reserve (NIAID, Tissue Culture Adapted) |-20°C or colder|ATCC?Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Vaccinia Virus, Western Reserve (NIAID, Tissue Culture Adapted), NR-2639."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Genomic DNA was isolated from a preparation of cell lysate and supernatant from African green monkey cells (Vero; ATCC ? CCL-81?) infected with vaccinia virus, Western Reserve (WR; NIAID, tissue culture adapted; BEI Resources NR-55).The WR (NIAID, tissue culture adapted) strain was derived from the original New York City Board of Health (NYCBH) strain by intracerebral passages in mice followed by tissue culture adaptation. It has been utilized in constructing vectors for gene expression and in producing viral proteins and DNA. The complete genomic sequence of vaccinia virus, WR has been determined (GenBank: AY243312).NR-2639 has been qualified for PCR applications by amplification of a sequence of at least 1000 bp. NR-2639 is not intended for use as a standard for quantitative PCR.Each vial contains 100 ?L of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0). The viral genomic DNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.

NR-55??Vaccinia virus, Western Reserve (NIAID, Tissue Culture Adapted) (Viruses)|Vaccinia virus|Western Reserve (NIAID, Tissue Culture Adapted) |-60°C|ATCC?Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Western Reserve (NIAID, Tissue Culture Adapted), NR-55."| Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. The Western Reserve (WR) strain of vaccinia virus (VACV) has been utilized in constructing vectors for gene expression and in producing viral proteins and DNA.Each vial contains approximately 1 mL of cell lysate and supernatant from African green monkey kidney (Vero) cells infected with VACV, WR (NIAID, tissue culture adapted).Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses [CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 5th ed, 2007].Additional information and tools are available at ViPR (Virus Pathogen Resource).