产品图片
货号/SKU
NR-2651
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-2651?? Genomic DNA from Escherichia coli, Strain CDC 9707 (Nucleic Acids)|Escherichia coli|Genomic DNA from Escherichia coli, Strain CDC 9707 |-20°C|ATCC?Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Escherichia coli, Strain CDC 9707, NR-2651."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Genomic DNA was isolated from a preparation of Escherichia coli (E. coli), strain CDC 9707, serotype O127:K63(B8). The bacterial preparation was produced by propagation of BEI Resources NR-98.
The enteropathogenic E. coli (EPEC) strain CDC 9707 was deposited at ATCC ? by the Communicable Disease Center, Chablee, Georgia in 1967. EPEC strains cause diarrheal outbreaks and chronic diarrhea, especially in infants. EPEC pathogenesis requires the expression of genes present both on the chromosome and on an adherence factor plasmid, pEAF. The complete sequence of the pEAF plasmid (also referred to as pB171) from EPEC strain B171 has been determined (68,817 bp; GenBank: AB024946).
NR-2651 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene as well as a virulence marker on the chromosome. The presence of pEAF has been confirmed by PCR amplification of two virulence markers.
Each vial contains 4-6 micrograms of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH approximately 7.4). The vial should be centrifuged prior to opening.
Genomic DNA was isolated from a preparation of Escherichia coli (E. coli), strain CDC 9707, serotype O127:K63(B8). The bacterial preparation was produced by propagation of BEI Resources NR-98.
The enteropathogenic E. coli (EPEC) strain CDC 9707 was deposited at ATCC ? by the Communicable Disease Center, Chablee, Georgia in 1967. EPEC strains cause diarrheal outbreaks and chronic diarrhea, especially in infants. EPEC pathogenesis requires the expression of genes present both on the chromosome and on an adherence factor plasmid, pEAF. The complete sequence of the pEAF plasmid (also referred to as pB171) from EPEC strain B171 has been determined (68,817 bp; GenBank: AB024946).
NR-2651 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene as well as a virulence marker on the chromosome. The presence of pEAF has been confirmed by PCR amplification of two virulence markers.
Each vial contains 4-6 micrograms of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH approximately 7.4). The vial should be centrifuged prior to opening.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. 基因组DNA从制剂中分离大肠杆菌(大肠杆菌),菌株CDC 9707 ,血清型O127:K63(B8).通过BEI资源NR-98繁殖产生细菌制剂. 肠致病性 e. 1967年,Coli (EPEC)菌株CDC 9707沉积在ATCC?的Chablee,格鲁吉亚,格鲁吉亚,1967年.EPEC菌株导致腹泻爆发和慢性腹泻,特别是在婴儿. EPEC发病机制需要表达在染色体上和粘附因子质粒上存在的基因表达.已经确定了来自EPEC菌株B171的PEAF质粒(也称为PB171)的完整序列(68,817 BP; GENBANK:AB024946). NR-2651已通过扩增约1500获得PCR应用16S核糖体RNA基因的BP以及染色体上的毒力标记物. PCR扩增的两个毒力标记证实了PEAF的存在. 每个小瓶在TE缓冲液中含有4-6微克的细菌基因组DNA(10mM Tris-HCl,1mM EDTA,pH约7.4) .在打开之前应该离心小瓶.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
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