NR-2650_来自大肠杆菌的基因组DNA,菌株H414-36/89(核酸)
产品图片
货号/SKU
NR-2650
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-2650?? Genomic DNA from Escherichia coli, Strain H414-36/89 (Nucleic Acids)|Escherichia coli|Genomic DNA from Escherichia coli, Strain H414-36/89 |-20°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Escherichia coli, Strain H414-36/89, NR-2650."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Genomic DNA was isolated from a preparation of Escherichia coli (E. coli), strain H414-36/89, serotype O91:H21.
The enterohemorrhagic E. coli (EHEC) strain H414-36/89 was isolated from a patient with hemorrhagic colitis in Germany. E. coli H414-36/89 and many other EHEC strains encode potent toxins, similar to those of Shigella dysenteriae, which can cause severe intestinal, kidney and central nervous system disease. The large plasmid of E. coli H414-36/89 carries one copy of the gene for Shiga-like toxin type II (SLT-II) and two copies of the gene for an SLT-II-variant-type toxin. E. coli H414 36/89 has been shown to be highly virulent in a mouse model of infection.
NR-2650 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene as well as amplification of a gene length virulence marker on the large plasmid.
Each vial contains 4-6 micrograms of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH approximately 7.4). The concentration is shown on the Certificate of Analysis. The vial should be centrifuged prior to opening.
Genomic DNA was isolated from a preparation of Escherichia coli (E. coli), strain H414-36/89, serotype O91:H21.
The enterohemorrhagic E. coli (EHEC) strain H414-36/89 was isolated from a patient with hemorrhagic colitis in Germany. E. coli H414-36/89 and many other EHEC strains encode potent toxins, similar to those of Shigella dysenteriae, which can cause severe intestinal, kidney and central nervous system disease. The large plasmid of E. coli H414-36/89 carries one copy of the gene for Shiga-like toxin type II (SLT-II) and two copies of the gene for an SLT-II-variant-type toxin. E. coli H414 36/89 has been shown to be highly virulent in a mouse model of infection.
NR-2650 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene as well as amplification of a gene length virulence marker on the large plasmid.
Each vial contains 4-6 micrograms of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH approximately 7.4). The concentration is shown on the Certificate of Analysis. The vial should be centrifuged prior to opening.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制之前的订单将被发送到发货前的批准. 基因组DNA从制备大肠杆菌(大肠杆菌),菌株H414- 36/89,血清型O91:H21. Enterohemorrhagic e. Coli (EHEC)菌株H414-36 / 89是从德国出血性结肠炎的患者分离的. e. COLI H414-36 / 89以及许多其他EHEC菌株编码有效的毒素,类似于志贺菌痢疾人的毒素,这可能导致严重的肠道,肾脏和中枢神经系统疾病. e的大质粒. COLI H414-36 / 89携带一种臭氧类毒素II型(SLT-II)的基因拷贝,以及用于SLT-II变异型毒素的基因的两份. e. Coli H414 36/89已被证明是一种在感染的小鼠模型中具有高度毒性. NR-2650通过扩增16s核糖体RNA基因的约1500bp,NR-2650已获得PCR应用以及大质粒上的基因长度毒力标志物的扩增. 每个小瓶在Te缓冲液中含有4-6微克细菌基因组DNA(10mM Tris-HCl,1mM EDTA,pH约7.4) .浓度显示在分析证书上.在打开之前应该离心小瓶.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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