菌株 A1122(核酸），NR-1_Vaccinia virus

NR-3040?? Genomic DNA from Yersinia pestis, Strain A1122 (Nucleic Acids)|Yersinia pestis|Genomic DNA from Yersinia pestis, Strain A1122 |-20°C or colder|CDCAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Yersinia pestis, Strain A1122, NR-3040."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Genomic DNA was isolated from a preparation of Yersinia pestis (Y. pestis), strain A1122.Y. pestis A1122 was isolated from a California ground squirrel (Spermophilus beecheyi) in California in 1939. It contains the pMT1/pFra and the pPCP1/pPla plasmids, but lacks the pCD1/pYV plasmid that is essential for virulence as well as the unstable pgm locus. The complete genome and plasmid sequences are available (GenBank: CP002956, CP002957 and CP002958).The presence of the pMT1/pFra and the pPCP1/pPla plasmids and the absence of the pCD1/pYV plasmid and the pgm locus in NR-3040 have been confirmed by Next Generation Sequencing. NR-3040 has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene.Each vial of lot 63815634 contains approximately 0.7 ?g to 1.5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 8.0). Each vial of lot 7513127 contains approximately 5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4). The vial should be centrifuged prior to opening.

NR-2644?? Genomic DNA from Yersinia pestis, Strain A1122 (Nucleic Acids)|Yersinia pestis|Genomic DNA from Yersinia pestis, Strain A1122 | -20°C or colder|ATCC?Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Yersinia pestis, Strain A1122, NR-2644."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Genomic DNA was extracted from a preparation of Yersinia pestis (Y. pestis), strain A1122. Y. pestis, strain A1122 was isolated from a California ground squirrel (Spermophilus beecheyi) in California in 1939. It contains two virulence plasmids: 1) pMT1 [pFra; ~ 110 kilobases (kb)], which encodes a murine toxin and capsular protein with anti-phagocytic activities, and 2) pPCP1 (pPla; ~ 9.5 kb monomer or ~ 19 kb dimer), which encodes a protease that facilitates the initial dissemination of the bacteria to the lymph nodes. Y. pestis, strain A1122 lacks the 70 kb plasmid that is essential for virulence as well as the unstable pgm locus. The complete genome sequence for Y. pestis, strain A1122 is available (GenBank: CP002956). The presence of the 110 kb and the 19 kb plasmids in NR-2644 has been confirmed by PCR amplification of a virulence marker on each plasmid. NR-2644 has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene. Each vial contains approximately 0.7 ?g to 1.5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 8). Each vial of lot 5107283 contains approximately 5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4). The vial should be centrifuged prior to opening.