NR-3040_来自鼠疫耶尔森菌的基因组DNA,菌株A1122(核酸）
产品图片
货号/SKU
NR-3040
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-3040?? Genomic DNA from Yersinia pestis, Strain A1122 (Nucleic Acids)|Yersinia pestis|Genomic DNA from Yersinia pestis, Strain A1122 |-20°C or colder|CDCAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Yersinia pestis, Strain A1122, NR-3040."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Genomic DNA was isolated from a preparation of Yersinia pestis (Y. pestis), strain A1122.
Y. pestis A1122 was isolated from a California ground squirrel (Spermophilus beecheyi) in California in 1939. It contains the pMT1/pFra and the pPCP1/pPla plasmids, but lacks the pCD1/pYV plasmid that is essential for virulence as well as the unstable pgm locus. The complete genome and plasmid sequences are available (GenBank: CP002956, CP002957 and CP002958).
The presence of the pMT1/pFra and the pPCP1/pPla plasmids and the absence of the pCD1/pYV plasmid and the pgm locus in NR-3040 have been confirmed by Next Generation Sequencing. NR-3040 has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene.
Each vial of lot 63815634 contains approximately 0.7 ?g to 1.5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 8.0). Each vial of lot 7513127 contains approximately 5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4). The vial should be centrifuged prior to opening.
Genomic DNA was isolated from a preparation of Yersinia pestis (Y. pestis), strain A1122.
Y. pestis A1122 was isolated from a California ground squirrel (Spermophilus beecheyi) in California in 1939. It contains the pMT1/pFra and the pPCP1/pPla plasmids, but lacks the pCD1/pYV plasmid that is essential for virulence as well as the unstable pgm locus. The complete genome and plasmid sequences are available (GenBank: CP002956, CP002957 and CP002958).
The presence of the pMT1/pFra and the pPCP1/pPla plasmids and the absence of the pCD1/pYV plasmid and the pgm locus in NR-3040 have been confirmed by Next Generation Sequencing. NR-3040 has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene.
Each vial of lot 63815634 contains approximately 0.7 ?g to 1.5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 8.0). Each vial of lot 7513127 contains approximately 5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4). The vial should be centrifuged prior to opening.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上的订单将在发货前发送到NIAID以批准. 基因组DNA从yersinia pestis（Y.pestis）的制备中分离出来的菌株A1122. y. Pestis A1122于1939年从加利福尼亚州的加州地面鼠（ Permophilus Beecheyi ）分离出来.它含有PMT1 / PFRA和PPCP1 / PPLA质粒，但缺乏PCD1 / PYV质粒对毒力至根本是必不可少的，也是不稳定的 PGM 基因座.完整的基因组和质粒序列可用（Genbank： cp002956 ，CP002957 和 CP002958 ）. PMT1 / PFRA的存在和PPCP1 / PPLA质粒和不存在PCD1 / PYV质粒和NR中的 PGM 座位3040已被下一代测序确认. NR-3040通过扩增16S核糖体RNA基因的大约1500个碱基对的批准. MM Tris-HCl，1mM EDTA，pH?8.0）. Lot 7513127的每个小瓶中含有约5μg的细菌基因组DNA（10mM Tris-HCl，1mM EDTA，pH?7.4）.在打开之前应该离心小瓶.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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