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产品图片
货号/SKU
NR-2644
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-2644?? Genomic DNA from Yersinia pestis, Strain A1122 (Nucleic Acids)|Yersinia pestis|Genomic DNA from Yersinia pestis, Strain A1122 | -20°C or colder|ATCC?Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Yersinia pestis, Strain A1122, NR-2644."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Genomic DNA was extracted from a preparation of Yersinia pestis (Y. pestis), strain A1122.
Y. pestis, strain A1122 was isolated from a California ground squirrel (Spermophilus beecheyi) in California in 1939. It contains two virulence plasmids: 1) pMT1 [pFra; ~ 110 kilobases (kb)], which encodes a murine toxin and capsular protein with anti-phagocytic activities, and 2) pPCP1 (pPla; ~ 9.5 kb monomer or ~ 19 kb dimer), which encodes a protease that facilitates the initial dissemination of the bacteria to the lymph nodes. Y. pestis, strain A1122 lacks the 70 kb plasmid that is essential for virulence as well as the unstable pgm locus. The complete genome sequence for Y. pestis, strain A1122 is available (GenBank: CP002956).
The presence of the 110 kb and the 19 kb plasmids in NR-2644 has been confirmed by PCR amplification of a virulence marker on each plasmid. NR-2644 has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene.
Each vial contains approximately 0.7 ?g to 1.5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 8). Each vial of lot 5107283 contains approximately 5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4). The vial should be centrifuged prior to opening.
Genomic DNA was extracted from a preparation of Yersinia pestis (Y. pestis), strain A1122.
Y. pestis, strain A1122 was isolated from a California ground squirrel (Spermophilus beecheyi) in California in 1939. It contains two virulence plasmids: 1) pMT1 [pFra; ~ 110 kilobases (kb)], which encodes a murine toxin and capsular protein with anti-phagocytic activities, and 2) pPCP1 (pPla; ~ 9.5 kb monomer or ~ 19 kb dimer), which encodes a protease that facilitates the initial dissemination of the bacteria to the lymph nodes. Y. pestis, strain A1122 lacks the 70 kb plasmid that is essential for virulence as well as the unstable pgm locus. The complete genome sequence for Y. pestis, strain A1122 is available (GenBank: CP002956).
The presence of the 110 kb and the 19 kb plasmids in NR-2644 has been confirmed by PCR amplification of a virulence marker on each plasmid. NR-2644 has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene.
Each vial contains approximately 0.7 ?g to 1.5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 8). Each vial of lot 5107283 contains approximately 5 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4). The vial should be centrifuged prior to opening.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
从yersinia pestis(Y.pestis)的制备中提取基因组DNA,菌株A1122.
y. Pestis ,菌株A1122在1939年在加州的加州地鼠疫(Phermophilus Beecheyi)中分离出来.它含有两种毒力质粒:1)PMT1 [PFRA; ?110千碱基(KB)],用抗吞噬活性编码鼠毒素和荚膜蛋白,2)PPCP1(PPLA;?9.5kb单体或?19 kB二聚体),其编码促进初始传播的蛋白酶细菌到淋巴结. y. Pestis ,菌株A1122缺乏70kb质粒,对于毒力至根本是必不可少的,并且不稳定的 PGM 基因座. y的完整基因组序列. Pestis,应变A1122可用(Genbank: cp002956 ).
通过PCR扩增在每种质粒上的毒力标记物的PCR扩增证实了110kb和NR-2644中的19kb质粒. NR-2644通过扩增16S核糖体RNA基因的大约1500个碱基对已经获得了PCR应用.
在Te缓冲液中,每个小瓶含有约0.7μg至1.5μg的细菌基因组DNA(10mM Tris-HCl,1mM EDTA,pH?8).批次的每个小瓶5107283在Te缓冲液中含有约5μg的细菌基因组DNA(10mM Tris-HCl,1mM EDTA,pH?7.4).在打开之前应该离心小瓶.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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