MRA-69质粒pHRPGFPM2,用于转染恶性疟原虫(质粒/载体）
产品图片
货号/SKU
MRA-69
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
MRA-69?? Plasmid pHRPGFPM2, for Transfection in Plasmodium falciparum(Plasmid/Vectors)|Plasmodium falciparum|Plasmid pHRPGFPM2, for Transfection in Plasmodium falciparum|-80°C or colder|K HaldarAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pHRPGFPM2, for Transfection in Plasmodium falciparum, MRA-69, contributed by Kasturi Haldar."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
GFPM2 can be successfully expressed in blood stages of Plasmodium falciparum. It has the potential to be used as a reporter in a variety of studies ranging from gene expression, protein localization and targeting, as well as organellar dynamics in live parasites. A clone of GFPM2 was used to amplify the coding region by PCR and the resulting fragment was engineered to contain an NsiI site at the 5' end and a HindIII site at the 3' end. The introduction of the NsiI site caused an amino acid change of Ser to His. The plasmid pHRPCAT was obtained, CAT was excised and GFPM2 was cloned into the vector to generate pHRPGFPM2. This construct was then electroporated into Escherichia coli XL1-Blue and the plasmid purified using a standard CsCl gradient. The resulting size of the plasmid is 6.25 kilobases. The sequence has not been confirmed by BEI Resources.
VanWye, J. D. and K. Haldar. "Expression of Green Fluorescent Protein in Plasmodium falciparum." Mol. Biochem. Parasitol. 87 (1997): 225-229. PubMed: 9247934.
GFPM2 can be successfully expressed in blood stages of Plasmodium falciparum. It has the potential to be used as a reporter in a variety of studies ranging from gene expression, protein localization and targeting, as well as organellar dynamics in live parasites. A clone of GFPM2 was used to amplify the coding region by PCR and the resulting fragment was engineered to contain an NsiI site at the 5' end and a HindIII site at the 3' end. The introduction of the NsiI site caused an amino acid change of Ser to His. The plasmid pHRPCAT was obtained, CAT was excised and GFPM2 was cloned into the vector to generate pHRPGFPM2. This construct was then electroporated into Escherichia coli XL1-Blue and the plasmid purified using a standard CsCl gradient. The resulting size of the plasmid is 6.25 kilobases. The sequence has not been confirmed by BEI Resources.
VanWye, J. D. and K. Haldar. "Expression of Green Fluorescent Protein in Plasmodium falciparum." Mol. Biochem. Parasitol. 87 (1997): 225-229. PubMed: 9247934.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
GFPM2可以成功地表达疟原虫的血液阶段.它有可能用作来自基因表达，蛋白质定位和靶向的各种研究中的报告，以及活寄生虫中的细胞细胞动态. GFPM2的克隆用于通过PCR扩增编码区，并将所得片段工程化以含有在5'末端的 NSI I位点，A> Hin DIII位点3'结束. NSI I Site的引入导致SER的氨基酸变化.得到质粒脊髓氏蛋白，切除猫，并将GFPM2克隆到载体中以产生PHRPGFPM2.然后将该构建体电穿孔进入 XL1-Blue并使用标准CSCL梯度纯化的质粒.质粒的所得尺寸为6.25千碱基.序列尚未通过BEI资源确认.
Vanwye，J. D.和K. Haldar. “在疟原虫中的绿色荧光蛋白的表达”. 摩尔.生物学习.寄生醇. 87（1997）：225-229. PubMed：9247934.
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