MRA-448质粒pHHT-TK,用于转染恶性疟原虫(质粒/载体)
产品图片
货号/SKU
MRA-448
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
MRA-448?? Plasmid pHHT-TK, for Transfection in Plasmodium falciparum(Plasmid/Vectors)|Plasmodium falciparum|Plasmid pHHT-TK, for Transfection in Plasmodium falciparum|-20°C or colder|AF Cowman, MT Duraisingh, T TrigliaAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pHHT-TK, for Transfection in Plasmodium falciparum, MRA-448, contributed by Alan F. Cowman, Manoj T. Duraisingh and Tony Triglia."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Thymidine kinase (TK) was isolated from herpes simplex virus. Plasmid pHHT-TK was designed for use in double knockout selection in Plasmodium falciparum (P. falciparum). Genetic markers include ampR in Escherichia coli, pro-drug WR99210 (wrR) and ganciclovir (gcvS) in Plasmodium. Ganciclovir sensitive when TK gene was present but insensitive when TK gene was replaced by double crossover events in P. falciparum. The resulting size of the plasmid is 7.662 kilobases.
Each vial of MRA-448 contains approximately 200 ng of plasmid DNA in TE buffer at a concentration of 10 ng/?L.
The depositors would like to acknowledge the IJP reference describing the TK system and to request this publication [Int. J. Parasitol. 32(1): 81-89] be cited for any publication using the pHHT-TK vector.
Duraisingh, M. T., T. Triglia and A. F. Cowman. "Negative Selection of Plasmodium falciparum Reveals Targeted Gene Deletion by Double Crossover Recombination." Int. J. Parasitol. 32 (2002): 81-89. PubMed: 11796125.
Reed, M. B., et al. "Pgh1 Modulates Sensitivity and Resistance to Multiple Antimalarials in Plasmondium falciparum." Nature 403 (2000): 906-909. PubMed: 10706290.
Thymidine kinase (TK) was isolated from herpes simplex virus. Plasmid pHHT-TK was designed for use in double knockout selection in Plasmodium falciparum (P. falciparum). Genetic markers include ampR in Escherichia coli, pro-drug WR99210 (wrR) and ganciclovir (gcvS) in Plasmodium. Ganciclovir sensitive when TK gene was present but insensitive when TK gene was replaced by double crossover events in P. falciparum. The resulting size of the plasmid is 7.662 kilobases.
Each vial of MRA-448 contains approximately 200 ng of plasmid DNA in TE buffer at a concentration of 10 ng/?L.
The depositors would like to acknowledge the IJP reference describing the TK system and to request this publication [Int. J. Parasitol. 32(1): 81-89] be cited for any publication using the pHHT-TK vector.
Duraisingh, M. T., T. Triglia and A. F. Cowman. "Negative Selection of Plasmodium falciparum Reveals Targeted Gene Deletion by Double Crossover Recombination." Int. J. Parasitol. 32 (2002): 81-89. PubMed: 11796125.
Reed, M. B., et al. "Pgh1 Modulates Sensitivity and Resistance to Multiple Antimalarials in Plasmondium falciparum." Nature 403 (2000): 906-909. PubMed: 10706290.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这一限制下订单将在发货前发送到NIAID进行批准. 胸苷激酶(TK)与单纯疱疹病毒分离.质粒pHHT-TK设计用于双截止选择,在疟原虫(p. falciparum)中.遗传标记包括在大肠杆菌中的AMPR,Pro-Bred WR99210(WRR)和Ganciclovir(GCVs)中的疟原虫.当TK基因被TK基因被 P中的双交叉事件所取代时,Ganciclovir敏感性但不敏感.恶魔饼.最终的质粒尺寸为7.662千碱基. 存款人喜欢确认描述TK系统的IJP参考并请求此出版物[int. J.Agarisitol. 32(1):81-89]用PHHT-TK载体引用任何出版物. Duraisingh,M.T.,T. Triglia和A. F. Cowman. “疟原虫的阴性选择疟原虫通过双交叉重组显示靶向基因缺失.” int. J.Arasisitol. 32(2002):81-89. PubMed:11796125. Reed,M. B.等人. “PGH1调节敏感性和抗性对多种抗疟药中的敏感性和抗性抗疟药 - 性质 403(2000):906-909. PubMed:10706290.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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