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产品图片
货号/SKU
MRA-272
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
MRA-272?? Plasmid pDS56-32/RBSII-CS27IVC-6XHis, for Transfection in Plasmodium falciparum(Plasmid/Vectors)|Plasmodium falciparum|Plasmid pDS56-32/RBSII-CS27IVC-6XHis, for Transfection in Plasmodium falciparum|-20°C or colder|P SinnisAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pDS56-32/RBSII-CS27IVC-6XHis, for Transfection in Plasmodium falciparum, MRA-272, contributed by Photini Sinnis."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
MRA-272 is purified plasmid DNA encoding a bacterial expression construct of synthetic Plasmodium falciparum circumsporozoite protein (PfCSP) domains with multiple CS protein repeats, codon-adjusted for Escherichia coli expression. The EcoRI-HindIII bacterial expression cassette includes RBSII which encodes CS27IVC-His6 (325 aa); coding region aa [27-123 -(NANPNVDP)3(NANP)21-300-411]-His6. The expressed peptide tends to be soluble. The resulting size of the plasmid is 4.369 kilobases.
Each vial of MRA-272 contains 50 ?L of purified plasmid DNA in buffer at a concentration of approximately 10 ng/?L. MRA-272 must be transformed into a suitable host (e.g. Escherichia coli DH5?) prior to use. Please consult appropriate protocols for bacterial transformation.
Cerami, C., et al. "The Basolateral Domain of the Hepatocyte Plasma Membrane Bears Receptors for the Circumsporozoite Protein of Plasmodium falciparum Sporozoites." Cell 70 (1992): 1021-1033. PubMed: 1326407.
Takacs, B. J. "Preparation of Clinical Grade Proteins Produced by Recombinant DNA Technologies." J. Immunol. Methods 143 (1991): 231-240. PubMed: 1940392.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
MRA-272 is purified plasmid DNA encoding a bacterial expression construct of synthetic Plasmodium falciparum circumsporozoite protein (PfCSP) domains with multiple CS protein repeats, codon-adjusted for Escherichia coli expression. The EcoRI-HindIII bacterial expression cassette includes RBSII which encodes CS27IVC-His6 (325 aa); coding region aa [27-123 -(NANPNVDP)3(NANP)21-300-411]-His6. The expressed peptide tends to be soluble. The resulting size of the plasmid is 4.369 kilobases.
Each vial of MRA-272 contains 50 ?L of purified plasmid DNA in buffer at a concentration of approximately 10 ng/?L. MRA-272 must be transformed into a suitable host (e.g. Escherichia coli DH5?) prior to use. Please consult appropriate protocols for bacterial transformation.
Cerami, C., et al. "The Basolateral Domain of the Hepatocyte Plasma Membrane Bears Receptors for the Circumsporozoite Protein of Plasmodium falciparum Sporozoites." Cell 70 (1992): 1021-1033. PubMed: 1326407.
Takacs, B. J. "Preparation of Clinical Grade Proteins Produced by Recombinant DNA Technologies." J. Immunol. Methods 143 (1991): 231-240. PubMed: 1940392.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这一限制下订单将在发货前发送到北欧以批准. 该试剂由贡献者认证.北京资源尚未确认或验证了这种材料. MRA-272是编码合成的细菌表达构建体的纯化质粒DNA,用于合成的疟原虫(PFCSP)域(PFCSP)域的细菌表达构建体多种Cs蛋白重复,对CONSCHERICHIA COLI 表达的密码子调整. eco RI- Hin DIII细菌表达盒包括编码CS27ivc-HIS6(325 AA)的RBSII;编码区域AA [27-123 - (NANPNVDP)3(NANP)21-300-411] -HIS6.表达的肽趋于可溶.质粒的所得尺寸为4.369千碱基. 浓度为约10ng /μl的缓冲液中的50μl纯化的质粒DNA. MRA-272必须在使用前转化为合适的宿主(例如,大肠杆菌Coli DH5?).请咨询合适的细菌转型方案. Cerami,C.等人. “肝细胞血浆膜的基底外立结构域承受了疟原虫的环孢子蛋白孢子蛋白孢子蛋白的受体.” 细胞 70(1992):1021-1033. PUBMED:1326407. TAGACS,B. J.“通过重组DNA技术制备临床级蛋白质.” j.免疫素.方法 143(1991):231-240. PubMed:1940392.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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