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产品图片

货号/SKU
MRA-662
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
MRA-662?? Hybridoma b5 Anti-Plasmodium berghei Phosphoprotein, Homologue to HSP70 Co-Chaperone (HIP, p48)(Cell Banks)|Plasmodium berghei|Hybridoma b5 Anti-Plasmodium berghei Phosphoprotein, Homologue to HSP70 Co-Chaperone (HIP, p48)|-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer|MF WiserAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma b5 Anti-Plasmodium berghei Phosphoprotein, Homologue to HSP70 Co-Chaperone (HIP, p48), MRA-662, contributed by Mark F. Wiser."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The murine hybridoma cell line, b5, was generated by the fusion of mouse myeloma cells (P3X63.Ag8.6.5.3) with splenocytes from mice immunized with purified 58 kDa phosphoprotein from Plasmodium berghei. This reagent was authenticated by the contributor and tested for mycoplasma.
Each vial of MRA-662 contains approximately 0.5 mL of hybridoma cells (5 × 106 cells/mL) in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO).
Hybridoma b5 produces monoclonal antibody (isotype IgG1) that specifically recognizes a 58 kDa phosphoprotein identified as a Plasmodium homolog to the heat shock protein 70 (HSP70) co-chaperone (known either as HIP or p48), and reacts with the protein from all rodent Plasmodium species.
Wiser, M. F. and B. Plitt. "Plasmodium berghei, P. chabaudi, and P. falciparum: Similarities in Phosphoproteins and Protein Kinase Activities and Their Stage Specific Expression." Exp. Parasitol. 64 (1987): 328-335. PubMed: 3315732.
Wiser, M. F., et al. "Further Characterization of a 58 kDa Plasmodium berghei Phosphoprotein as a Cochaperone." Mol. Biochem. Parasitol. 83 (1996): 25-33. PubMed: 9010839.
The murine hybridoma cell line, b5, was generated by the fusion of mouse myeloma cells (P3X63.Ag8.6.5.3) with splenocytes from mice immunized with purified 58 kDa phosphoprotein from Plasmodium berghei. This reagent was authenticated by the contributor and tested for mycoplasma.
Each vial of MRA-662 contains approximately 0.5 mL of hybridoma cells (5 × 106 cells/mL) in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO).
Hybridoma b5 produces monoclonal antibody (isotype IgG1) that specifically recognizes a 58 kDa phosphoprotein identified as a Plasmodium homolog to the heat shock protein 70 (HSP70) co-chaperone (known either as HIP or p48), and reacts with the protein from all rodent Plasmodium species.
Wiser, M. F. and B. Plitt. "Plasmodium berghei, P. chabaudi, and P. falciparum: Similarities in Phosphoproteins and Protein Kinase Activities and Their Stage Specific Expression." Exp. Parasitol. 64 (1987): 328-335. PubMed: 3315732.
Wiser, M. F., et al. "Further Characterization of a 58 kDa Plasmodium berghei Phosphoprotein as a Cochaperone." Mol. Biochem. Parasitol. 83 (1996): 25-33. PubMed: 9010839.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送至NIAID进行批准. 鼠杂交瘤细胞系B5是由小鼠骨髓瘤细胞的融合(P3X63.AG8.5.3)产生的,用纯化的58kDa磷蛋白免疫的小鼠与纯化的58kDa磷蛋白免疫的小鼠中的脾细胞Plasmodium perghei .该试剂由贡献者认证并对支原体进行测试. 每个小瓶MRA-662含有约0.5ml的细胞培养中的杂交瘤细胞(5×10 6 细胞/ ml)补充有10%二甲基磺酰氧化物(DMSO)的培养基. 杂交瘤B5产生单克隆抗体(同种型IgG1),其特异性识别为热休克蛋白70鉴定为疟原虫的同源物的58kDa磷蛋白(HSP70)共伴侣(髋关节或P48),并与来自所有啮齿动物的蛋白质疟原虫等物种反应. Wiser,MF和B. Plitt. “ Plasmodium berghei , p.chabaudi ,和 p. falciparum :磷蛋白质和蛋白激酶活性的相似性及其阶段特异性表达.” Exp.寄生醇. 64(1987):328-335. PubMed:3315732. Wiser,M. F.等人. “进一步表征58kDa 疟原虫磷蛋白作为Cochaperone.” 摩尔.生物学习.寄生醇. 83(1996):25-33. PUBMED:9010839.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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