Modified Vaccinia Ankara (MVA)(Viruses)

MRA-332??Plasmodium yoelii, Strain YM, cDNA Library (Libraries)|Plasmodium yoelii|Plasmodium yoelii, Strain YM, cDNA Library|-80°C|JH AdamsAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium yoelii, Strain YM, cDNA Library, MRA-332, contributed by John H. Adams."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material. Total RNA samples were isolated from mixed-stage Plasmodium yoelii (P. yoelii), strain YM infected erythrocytes by the acidic guanidinium-phenol chloroform method. The poly A+ RNA was isolated by Amersham Pharmacia mRNA purification kit using oligo-dT column chromatography. Directional cDNA libraries were constructed by oligo d(T) priming of poly(A)+ RNA (4 mg) into NotI and SalI sites of lambda ZipLox vector using the Gibco BRL SuperScript Lambda System. The average size of the cDNA inserts in the library was between 1.0 and 1.5 kb. Colonies from E. coli DH10B(ZIP) harboring plasmid were obtained from lambda ZipLox clones by in vivo excision.

MRA-330?? Plasmodium falciparum Malayan Camp R+(Parasitic Protozoa)|Plasmodium falciparum| Plasmodium falciparum Malayan Camp R+| -80°C or colder|LH Miller, D BaruchAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, Strain Malayan Camp R⁺, MRA-330, contributed by Louis H. Miller and Dror Baruch.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Plasmodium falciparum (P. falciparum), strain Malayan Camp R⁺ was isolated by selection during in vitro growth of the Malayan Camp strain. The parent Malayan Camp strain was first isolated in Malaysia from a soldier who was given proguanil treatment, then established in Aotus monkeys and adapted to in vitro growth in human red blood cells.

P. falciparum, strain Malayan Camp R⁺ was deposited as having a rosetting (R⁺) phenotype; and reported as knob-positive (K⁺). Strain Malayan Camp has been reported as resistant to proguanil and cycloguanil. The complete genome of P. falciparum, strain CAMP/Malaysia has been sequenced (GenBank: AOPU00000000).

Each vial contains approximately 0.5 mL of P. falciparum-infected human blood in Glycerolyte 57 solution (1:5).

MRA-331G?? Genomic DNA from Plasmodium falciparum, Santa Lucia(Nucleic Acids)|Plasmodium falciparum|Genomic DNA from Plasmodium falciparum, Santa Lucia|-20°C or colder|LH Miller, D BaruchAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Plasmodium falciparum, Strain Santa Lucia, MRA-331G, contributed by Louis H. Miller and Dror Baruch.”|

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was extracted from a preparation of Plasmodium falciparum (P. falciparum), strain Santa Lucia.

P. falciparum, strain Santa Lucia (also referred to as St. Lucia) was isolated in 1975 from the blood of a patient at the Santa Lucia Clinic in El Salvador. Strain Santa Lucia was established in Aotus trivirgatus monkeys, and later passaged in vitro in human red blood cells. The Santa Lucia strain is reported to have a knob-positive (K⁺) phenotype. The complete genome of P. falciparum, strain Santa Lucia has been sequenced (GenBank: ABHA00000000).

MRA-331G has been qualified for PCR applications by amplification of approximately 900 base pairs of the merozoite surface protein 2 (MSP2) gene.

Each vial of MRA-331G contains approximately 0.5 ?g of genomic DNA at a concentration of 10 ?g per mL in TE buffer (10 mM Tris-HCl and 0.5 mM EDTA, pH 9). The vial should be centrifuged prior to opening.

MRA-331?? Plasmodium falciparum, Santa Lucia(Parasitic Protozoa)|Plasmodium falciparum| Plasmodium falciparum, Santa Lucia| -80°C or colder|LH Miller, D BaruchAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, Strain Santa Lucia, MRA-331, contributed by Louis H. Miller and Dror Baruch.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Plasmodium falciparum (P. falciparum), strain Santa Lucia was isolated in 1975 from the blood of a patient at the Santa Lucia Clinic in El Salvador. Strain Santa Lucia was established in Aotus trivirgatus monkeys, and later passaged in vitro in human red blood cells.

The Santa Lucia strain is reported to have a knob-positive (K⁺) phenotype. The complete genome of P. falciparum, strain Santa Lucia has been sequenced (GenBank: ABHA00000000).

Each vial contains approximately 0.5 mL of P. falciparum-infected human blood in Glycerolyte 57 solution (1:5).

MRA-328?? Plasmodium falciparum Malayan Camp K-(Parasitic Protozoa)|Plasmodium falciparum| Plasmodium falciparum Malayan Camp K-| -80°C or colder|LH Miller, D BaruchAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, Strain Malayan Camp K^-, MRA-328, contributed by Louis H. Miller and Dror Baruch.”|

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Plasmodium falciparum (P. falciparum), strain Malayan Camp K^- was isolated by long-term in vitro growth of the Malayan Camp strain. The parent Malayan Camp strain was first isolated in Malaysia from a soldier who was given proguanil treatment, then established in Aotus monkeys and adapted to in vitro growth in human red blood cells.

P. falciparum, strain Malayan Camp K^- was deposited as having a knobless (K^-) phenotype; and reported as non-rosetting and non-cytoadherent (R^-C^-). Strain Malayan Camp has been reported as resistant to proguanil and cycloguanil. The complete genome of P. falciparum, strain CAMP/Malaysia has been sequenced (GenBank: AOPU00000000).

Each vial contains approximately 0.5 mL of P. falciparum-infected human blood in Glycerolyte 57 solution (1:5).

MRA-329G?? Genomic DNA from Plasmodium falciparum Malayan Camp R-(Nucleic Acids)|Plasmodium falciparum| Genomic DNA from Plasmodium falciparum Malayan Camp R-|-20°C or colder|LH Miller, D BaruchAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Plasmodium falciparum, Strain Malayan Camp R^-, MRA-329G, contributed by Louis H. Miller and Dror Baruch.”|

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was extracted from a preparation of Plasmodium falciparum (P. falciparum), strain Malayan Camp R^-.

P. falciparum, strain Malayan Camp R^- was isolated by selection during in vitro growth of the Malayan Camp strain. The parent Malayan Camp strain was first isolated in Malaysia from a soldier who was given proguanil treatment, then established in Aotus monkeys and adapted to in vitro growth in human red blood cells. P. falciparum, strain Malayan Camp R^- was deposited as having a non-rosetting (R^-) phenotype; and reported as knob-positive (K⁺). Strain Malayan Camp has been reported as resistant to proguanil and cycloguanil. The complete genome of P. falciparum, strain CAMP/Malaysia has been sequenced (GenBank: AOPU00000000).

MRA-329G has been qualified for PCR applications by amplification of approximately 900 base pairs of the merozoite surface protein 2 (MSP2) gene.

Each vial of MRA-329G contains approximately 0.5 ?g of genomic DNA at a concentration of 10 ?g per mL in TE buffer (10 mM Tris-HCl and 0.5 mM EDTA, pH 9). The vial should be centrifuged prior to opening.

MRA-329?? Plasmodium falciparum Malayan Camp R-(Parasitic Protozoa)|Plasmodium falciparum| Plasmodium falciparum Malayan Camp R-| -80°C or colder|LH Miller, D BaruchAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, Strain Malayan Camp R^-, MRA-329, contributed by Louis H. Miller and Dror Baruch.”|

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Plasmodium falciparum (P. falciparum), strain Malayan Camp R^- was isolated by selection during in vitro growth of the Malayan Camp strain. The parent Malayan Camp strain was first isolated in Malaysia from a soldier who was given proguanil treatment, then established in Aotus monkeys and adapted to in vitro growth in human red blood cells.

P. falciparum, strain Malayan Camp R^- was deposited as having a non-rosetting (R^-) phenotype; and reported as knob-positive (K⁺). Strain Malayan Camp has been reported as resistant to proguanil and cycloguanil. The complete genome of P. falciparum, strain CAMP/Malaysia has been sequenced (GenBank: AOPU00000000).

Each vial contains approximately 0.5 mL of P. falciparum-infected human blood in Glycerolyte 57 solution (1:5).

MRA-330G?? Genomic DNA from Plasmodium falciparum?Malayan Camp R+(Nucleic Acids)|Plasmodium falciparum|Genomic DNA from Plasmodium falciparum?Malayan Camp R+|-20°C or colder|LH Miller, D BaruchAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Plasmodium falciparum, Strain Malayan Camp R⁺, MRA-330G, contributed by Louis H. Miller and Dror Baruch.”|

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was extracted from a preparation of Plasmodium falciparum (P. falciparum), strain Malayan Camp R⁺.

P. falciparum, strain Malayan Camp R⁺ was isolated by selection during in vitro growth of the Malayan Camp strain. The parent Malayan Camp strain was first isolated in Malaysia from a soldier who was given proguanil treatment, then established in Aotus monkeys and adapted to in vitro growth in human red blood cells. P. falciparum, strain Malayan Camp R⁺ was deposited as having a rosetting (R⁺) phenotype and reported as knob-positive (K⁺). Strain Malayan Camp has been reported as resistant to proguanil and cycloguanil. The complete genome of P. falciparum, strain CAMP/Malaysia has been sequenced (GenBank: AOPU00000000).

MRA-330G has been qualified for PCR applications by amplification of approximately 900 base pairs of the merozoite surface protein 2 (MSP2) gene.

Each vial of MRA-330G contains approximately 0.5 ?g of genomic DNA at a concentration of 10 ?g per mL in TE buffer (10 mM Tris-HCl and 0.5 mM EDTA, pH 9). The vial should be centrifuged prior to opening.

MRA-327G?? Genomic DNA from Plasmodium falciparum, ITG-2F6(Nucleic Acids)|Plasmodium falciparum|Genomic DNA from Plasmodium falciparum, ITG-2F6|-20°C or colder|LH Miller, D BaruchAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Plasmodium falciparum, Strain ITG-2F6, MRA-327G, contributed by Louis H. Miller and Dror Baruch.”|

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was extracted from a preparation of Plasmodium falciparum (P. falciparum), strain ITG-2F6.

P. falciparum, strain ITG-2F6 was derived from clone It.G2 after it was passaged through human red blood cells. Clone It.G2 was derived via cell culture from isolate Ituxi 084, which was isolated in 1979 at the Ituxi River, Brazil. P. falciparum, strain ITG-2F6 is reported to have a knob-positive (K⁺) phenotype and sensitivity to pyrimethamine.

MRA-327G has been qualified for PCR applications by amplification of approximately 900 base pairs of the merozoite surface protein 2 (MSP2) gene.

Each vial of MRA-327G contains approximately 0.5 ?g of genomic DNA at a concentration of 10 ?g per mL in TE buffer (10 mM Tris-HCl and 0.5 mM EDTA, pH 9). The vial should be centrifuged prior to opening.

MRA-327??Plasmodium falciparum, ITG-2F6(Parasitic Protozoa)|Plasmodium falciparum|ITG-2F6|-80°C or colder|LH Miller, D BaruchAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, Strain ITG-2F6, MRA-327, contributed by Louis H. Miller and Dror Baruch."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Plasmodium falciparum (P. falciparum), strain ITG-2F6 was derived from clone It.G2 after it was passaged through human red blood cells. Clone It.G2 was derived via cell culture from isolate Ituxi 084, which was isolated in 1979 at the Ituxi River, Brazil. P. falciparum, strain ITG-2F6 is reported to have a knob positive (K+) phenotype and sensitivity to pyrimethamine. Each vial contains approximately 0.5 mL of P. falciparum infected human blood in Glycerolyte 57 solution (1:5).