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产品图片
货号/SKU
MRA-594
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
MRA-594?? Anopheles gambiae ZANU(Vectors)|Anopheles gambiae|Anopheles gambiae ZANU|Room temperature|H Ranson, FH CollinsAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Anopheles gambiae, Strain ZANU, MRA-594, contributed by Hilary Ranson and Frank H. Collins."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Anopheles gambiae, strain ZANU is the only stock of A. gambiae known (2002) to express increased resistance to DDT due to elevated metabolism (according to H. Ranson). Original ZANDS stock was selected with DDT in the larval stage. ZAN/U was selected in the adult stage. MR4 performs selection on L4s. Selection can be performed on adults using 4% DDT WHO papers. This stock contains a glutathionine-S-transferase mechanism and elevated beta-esterase activity. Identified for species by morphological standard every 5 generations. Treat with DDT as L4 at 0.4 mg/liter for 24 hours, every 5 generations (food must be included in the container during the treatment). Record the number of dead and survivors after treatment. ID 25 individuals for species by PCR using A. gambiae complex primers every 10 generations.
MRA-594 contains a suitable number of eggs to establish a stock. Eggs are provided on damp filter paper and should be hatched immediately upon receipt.
Applications for MRA-594 include DDT resistance conferred by increased glutathione S transferase activity.
Lines, J. D. and N. S. Nassor. "DDT Resistance in Anopheles gambiae Declines with Mosquito Age." Med. Vet. Entomol. 5 (1991): 261-265. PubMed: 1768918.
Hemingway, J. "Biochemical Studies on Malathion Resistance in Anopheles arabiensis from Sudan." Trans. R. Soc. Trop. Med. Hyg. 77 (1983): 477-480. PubMed: 6636275.
Prapanthadara, L. A., et al. "Characterization of the Major Form of Glutathione Transferase in the Mosquito Anopheles dirus A." Biochem. Soc. Trans. 23 (1995): 81S. PubMed: 7758801.
Anopheles gambiae, strain ZANU is the only stock of A. gambiae known (2002) to express increased resistance to DDT due to elevated metabolism (according to H. Ranson). Original ZANDS stock was selected with DDT in the larval stage. ZAN/U was selected in the adult stage. MR4 performs selection on L4s. Selection can be performed on adults using 4% DDT WHO papers. This stock contains a glutathionine-S-transferase mechanism and elevated beta-esterase activity. Identified for species by morphological standard every 5 generations. Treat with DDT as L4 at 0.4 mg/liter for 24 hours, every 5 generations (food must be included in the container during the treatment). Record the number of dead and survivors after treatment. ID 25 individuals for species by PCR using A. gambiae complex primers every 10 generations.
MRA-594 contains a suitable number of eggs to establish a stock. Eggs are provided on damp filter paper and should be hatched immediately upon receipt.
Applications for MRA-594 include DDT resistance conferred by increased glutathione S transferase activity.
Lines, J. D. and N. S. Nassor. "DDT Resistance in Anopheles gambiae Declines with Mosquito Age." Med. Vet. Entomol. 5 (1991): 261-265. PubMed: 1768918.
Hemingway, J. "Biochemical Studies on Malathion Resistance in Anopheles arabiensis from Sudan." Trans. R. Soc. Trop. Med. Hyg. 77 (1983): 477-480. PubMed: 6636275.
Prapanthadara, L. A., et al. "Characterization of the Major Form of Glutathione Transferase in the Mosquito Anopheles dirus A." Biochem. Soc. Trans. 23 (1995): 81S. PubMed: 7758801.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这个限制上订单将在发货前发送到NIAID进行批准. anopheles gambiae ，rucatedzanu是 a的唯一库存.冈菊（2002）已知（2002）由于代谢升高，表达对DDT的增加抗性（根据H. Ranson）.在幼虫阶段的DDT中选择了原始Zands股票. Zan / U在成人阶段选择. MR4在L4S上执行选择.可以使用4％DDT文件对成人进行选择.该股票含有谷胱甘肽-S-转移酶机制和升高的β-酯酶活性.每5代通过形态标准鉴定物种.用DDT作为L4治疗0.4 mg /升24小时，每5代（食物必须在处理过程中包含在容器中）.在治疗后记录死亡和幸存者的数量.使用 a通过PCR为物种ID 25个体.冈比亚每10代的复杂引物. Mra-594包含合适数量的鸡蛋来建立股票.鸡蛋提供潮湿的滤纸，并在收到后立即孵化. MRA-594的应用包括通过增加的谷胱甘肽转移酶活性赋予DDT电阻. 线条，J. D.和N. S. Nasor. “DDT抗性在 anophelesGambiae 与蚊子年龄下降.” Med.兽医. entomol. 5（1991）：261-265. PubMed：1768918. Hemingway，J.“来自苏丹的 anopheles Arabiensis的恶性抗性生物化学研究”. 跨. R. SoC.倾斜.医学. HYG. 77（1983）：477-480. PubMed：6636275. Prapanthadara，L. A.等人. “蚊子中谷胱甘肽转移酶的主要形式的表征蝙蝠紫外线 A.” Biochem. SOC. Trans. 23（1995）：81s. PUBMED：7758801.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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