MRA-658杂交瘤13.5抗疟原虫红细胞膜蛋白[Pc(em)-93](细胞库）
产品图片
货号/SKU
MRA-658
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
MRA-658?? Hybridoma 13.5 Anti-Plasmodium chabaudi Erythrocyte Membrane Protein [Pc(em)-93](Cell Banks)|Plasmodium chabaudi|Hybridoma 13.5 Anti-Plasmodium chabaudi Erythrocyte Membrane Protein [Pc(em)-93]|-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer|MF WiserAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 13.5 Anti-Plasmodium chabaudi Erythrocyte Membrane Protein [Pc(em)-93], MRA-658, contributed by Mark F. Wiser."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The murine hybridoma cell line, 13.5, was generated by the fusion of mouse myeloma cells (P3X63.Ag8.6.5.3) with splenocytes from mice immunized with a purified, glutamate-rich, 93-kDa erytrocyte-membrane-associated protein from Plasmodium chabaudi (P. chabaudi), Pc(em)-93. This reagent was authenticated by the contributor and tested for mycoplasma.
Each vial of MRA-658 contains approximately 0.5 mL of hybridoma cells (5 × 106 cells/mL) in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO).
Hybridoma 13.5 produces monoclonal antibody (isotype IgG1) that specifically recognizes an approximately 90-kDa P. chabaudi phosphoprotein associated with the erytrocyte membrane. The antigen recognized by the 13.5 antibody is an excellent marker for P. chabaudi-infected erythrocyte membranes and has been used to study protein export in infected erythrocytes.
Wiser, M. F., M. B. Leible and B. Plitt. "Acidic Phosphoproteins Associated with the Host Erythrocyte Membrane of Erythrocytes Infected with Plasmodium berghei and P. chabaudi." Mol. Biochem. Parasitol. 27 (1988): 11-22. PubMed: 3278220.
Wiser, M. F., et al. "Plasmodium chabaudi: Immunogenicity of a Highly Antigenic Glutamate-Rich Protein." Exp. Parasitol. 85 (1997): 43-54. PubMed: 9024201.
Giraldo, L. E., et al. "Molecular Characterization of a Plasmodium chabaudi Erythrocyte Membrane-Associated Protein with Glutamate-Rich Tandem Repeats." J. Eukaryot. Microbiol. 45 (1998): 528-534. PubMed: 9783454.
The murine hybridoma cell line, 13.5, was generated by the fusion of mouse myeloma cells (P3X63.Ag8.6.5.3) with splenocytes from mice immunized with a purified, glutamate-rich, 93-kDa erytrocyte-membrane-associated protein from Plasmodium chabaudi (P. chabaudi), Pc(em)-93. This reagent was authenticated by the contributor and tested for mycoplasma.
Each vial of MRA-658 contains approximately 0.5 mL of hybridoma cells (5 × 106 cells/mL) in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO).
Hybridoma 13.5 produces monoclonal antibody (isotype IgG1) that specifically recognizes an approximately 90-kDa P. chabaudi phosphoprotein associated with the erytrocyte membrane. The antigen recognized by the 13.5 antibody is an excellent marker for P. chabaudi-infected erythrocyte membranes and has been used to study protein export in infected erythrocytes.
Wiser, M. F., M. B. Leible and B. Plitt. "Acidic Phosphoproteins Associated with the Host Erythrocyte Membrane of Erythrocytes Infected with Plasmodium berghei and P. chabaudi." Mol. Biochem. Parasitol. 27 (1988): 11-22. PubMed: 3278220.
Wiser, M. F., et al. "Plasmodium chabaudi: Immunogenicity of a Highly Antigenic Glutamate-Rich Protein." Exp. Parasitol. 85 (1997): 43-54. PubMed: 9024201.
Giraldo, L. E., et al. "Molecular Characterization of a Plasmodium chabaudi Erythrocyte Membrane-Associated Protein with Glutamate-Rich Tandem Repeats." J. Eukaryot. Microbiol. 45 (1998): 528-534. PubMed: 9783454.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送至NIAID进行批准. 鼠杂交瘤细胞系13.5，由小鼠骨髓瘤细胞（p3x63.ag8.6.3.3）的融合产生（p3x63.ag8.5.3），用纯化的，富含纯化的小鼠免疫小鼠的脾细胞产生93 -KDA冰纤维 - 膜相关蛋白来自疟原虫chabaudi（p. chabaudi），pc（em）-93.该试剂由贡献者认证并对支原体进行测试. 细胞培养中的每个小瓶含有大约0.5ml的杂交瘤细胞（5×10 6 细胞/ ml）补充有10％二甲基磺砜（DMSO）的培养基. 杂交瘤13.5产生特异性识别约90kDa的单克隆抗体（同种型IgG1）. chabaudi 与红细胞膜相关的磷蛋白.由13.5抗体识别的抗原是 p的优异标记物. Chabaudi - 植物的红细胞膜，已用于研究感染的红细胞中的蛋白质出口. Wiser，M. F.，M. B.LEALE和B. PLITT. “与宿主红细胞膜相关的酸性磷蛋白感染的红细胞感染疟原虫（Pergheii）和 p. chabaudi .” 摩尔.生物学习.寄生醇. 27（1988）：11-22. PubMed：3278220. Wiser，M. F.等人. “疟原虫（Chabaudi）：高抗原谷氨酸富含蛋白质的免疫原性.” Exp.寄生酚. 85（1997）：43-54. PubMed：9024201. Giraldo，L. E.等. “具有富含谷氨酸的串联重复的红细胞膜相关蛋白的Chabaudi（Chabaudi）的分子表征.” j.真核生物.微生物. 45（1998）：528-534. PubMed：9783454.
厂牌介绍
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