带有 C 末端组氨酸标签的武汉-Hu-1

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-53800?? Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C to -80°C|Sino BiologicalAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-53800.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724390) was produced by transfection in human embryonic kidney HEK293 cells and purified. NR-53800 lacks the signal sequence, contains 223 residues of the SARS-CoV-2 spike glycoprotein RBD (amino acid residues R319 to F541) and features a C-terminal poly-histidine tag. NR-53800 has a theoretical molecular weight of 26,540 daltons. The biological activity of NR-53800 was measured by its binding ability in a functional ELISA, in which immobilized human ACE2 protein (Fc tag) (Sino Biological 10108-H05H) (100 ?L/well) can bind NR-53800; the half maximal effective concentration (EC₅₀) of NR-53800 is 20 to 60 ng/mL. Each vial contains approximately 50 ?g of purified recombinant protein in phosphate buffered saline (PBS, pH 7.4). Note: NR-53800 was not lyophilized. Additional information and tools are available at ViPR (Virus Pathogen Resource). For more information please visit the Sino Biological website.

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept： YP_009724390 ”是通过在人胚胎肾HEK293细胞中转染而产生的，并纯化. NR-53800缺少信号序列，含有223个残基的SARS-COV-2穗糖蛋白RBD（氨基酸残基R319至F541），并具有C末端多组氨酸标签. NR-53800具有26,540道尔顿的理论分子量. NR-53800的生物活性通过其功能性ELISA的结合能力测量，其中固定化的人ACE2蛋白（FC标签）（SINO生物10108-H05H）（100μL/孔）可以结合NR-53800; NR-53800的半最大有效浓度（EC 50 ）为20至60ng / ml. 每个小瓶在磷酸盐缓冲盐水中含有大约50μg的纯化的重组蛋白（PBS，pH7.4）. 注意：NR-53800没有冻干. vipr （病毒病原体资源）提供了附加信息和工具. 有关更多信息，请访问 Sino Biological网站.

厂牌介绍

BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。

除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。

BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。

品牌标识

产品关键词

来自 HEK293 细胞(蛋白质）的重组，NR-1_Vaccinia virus

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NR-53799来自SARS相关冠状病毒2的刺突糖蛋白S2胞外域(ECD),带有C末端组氨酸标签的武汉-Hu-1,来自杆状病毒(蛋白质）的重组体

NR-53799_ Spike Glycoprotein S2 Extracellular Domain (ECD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)

产品图片

产品英文名称

产品别名

NR-53799_ Spike Glycoprotein S2 Extracellular Domain (ECD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)
NR-53799 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S2 胞外域 (ECD)，带有 C 末端组氨酸标签的武汉-Hu-1，来自杆状病毒（蛋白质）的重组体

货号/SKU

NR-53799

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

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支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-53799?? Spike Glycoprotein S2 Extracellular Domain (ECD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S2 Extracellular Domain (ECD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus|-20°C to -80°C|Sino BiologicalAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S2 Extracellular Domain (ECD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus, NR-53799.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike glycoprotein S2 extracellular domain (ECD) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724390) was produced by transfection in insect cells using a baculovirus expression system and purified. NR-53799 lacks the signal sequence, contains 528 residues of the SARS-CoV-2 spike glycoprotein S2 ECD (amino acid residues S686 to P1213) and features a C-terminal poly-histidine tag. NR-53799 has a theoretical molecular weight of 59,370 daltons. Each vial contains approximately 50 ?g of purified recombinant protein in phosphate buffered saline (PBS, pH 7.0) supplemented with 2% glycerol. Note: NR-53799 was not lyophilized. Additional information and tools are available at ViPR (Virus Pathogen Resource). For more information please visit the Sino Biological website.

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关冠状病毒2（SARS-COV-2）的穗糖蛋白S2细胞外结构域（ECD）的重组形式，武汉-U-1（Genpept： YP_009724390 ”通过使用杆状病毒表达系统转染并纯化来生产昆虫细胞. NR-53799缺少信号序列，含有528个残基的SARS-COV-2穗糖蛋白S2 ECD（氨基酸残基S686至P1213），并具有C末端多组氨酸标签. NR-53799具有59,370道尔顿的理论分子量. 每个小瓶在补充有2％甘油的磷酸盐缓冲盐水（PBS，pH7.0）中含有大约50μg的纯化的重组蛋白. 注意：NR-53799未冻干. vipr （病毒病原体资源）提供了附加信息和工具. 有关更多信息，请访问 Sino Biological网站.

厂牌介绍

品牌标识

产品关键词

来自杆状病毒(蛋白质）的重组体，NR-1_Vaccinia virus

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NR-53798来自SARS相关冠状病毒2的刺突糖蛋白S1结构域,带有C末端组氨酸标签的武汉-Hu-1,来自HEK293细胞的重组(蛋白质）

NR-53798_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-53798_ Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)
NR-53798 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域，带有 C 末端组氨酸标签的武汉-Hu-1，来自 HEK293 细胞的重组（蛋白质）

货号/SKU

NR-53798

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-53798?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C to -80°C|Sino BiologicalAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-53798.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724390) was produced by transfection in human embryonic kidney HEK293 cells and purified. NR-53798 lacks the signal sequence, contains 670 residues of the SARS-CoV-2 spike glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-53798 has a theoretical molecular weight of 76,500 daltons. The biological activity of NR-53798 was measured by its binding ability in a functional ELISA, in which immobilized human ACE2 protein (Fc tag) (Sino Biological 10108-H05H) at 2 ?g/mL (100 ?L/well) can bind NR-53798; the half maximal effective concentration (EC₅₀) of NR-53798 is 200 to 600 ng/mL. The biological activity of NR-53798 was also measured by its binding ability using biosensor analysis, in which human ACE2 protein (Fc tag) (Sino Biological 10108-H05H) can bind NR-53798; the affinity constant is 47.7 nM. Each vial contains approximately 50 ?g of purified recombinant protein in phosphate buffered saline (PBS, pH 7.4). Note: NR-53798 was not lyophilized. Additional information and tools are available at ViPR (Virus Pathogen Resource). For more information please visit the Sino Biological website.

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept： YP_009724390 ”并纯化. NR-53798缺乏信号序列，含有670个残基的SARS-COV-2穗糖蛋白（氨基酸残基V16至R685），并具有C末端多组氨酸标签. NR-53798具有76,500道尔顿的理论分子量. NR-53798的生物活性通过其官能团中的结合能力测量，其中固定化的人ACE2蛋白（Fc标签）（SINO生物10108-H05H）在2μg/ mL（100μl/孔）中可以结合NR- 53798; NR-53798的半最大有效浓度（EC 50 ）为200至600 ng / ml. NR-53798的生物活性也通过其使用生物传感器分析的结合能力来测量，其中人ACE2蛋白（FC标签）（SINO生物10108-H05H）可以结合NR-53798;亲和力常数为47.7 nm. 每个小瓶在磷酸盐缓冲盐水中含有大约50μg的纯化的重组蛋白（PBS，pH7.4）. 注意：NR-53798没有冻干. vipr （病毒病原体资源）提供了附加信息和工具. 有关更多信息，请访问 Sino Biological网站.

厂牌介绍

品牌标识

产品关键词

NR-53798 来自 SARS 相关冠状病毒 2 的刺突糖蛋白 S1 结构域

来自 HEK293 细胞的重组(蛋白质），NR-1_Vaccinia virus

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NR-53797_ Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)

产品图片

产品英文名称

产品别名

NR-53797_ Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)
NR-53797 来自 SARS 相关冠状病毒 2 的核衣壳蛋白，带有 C 末端组氨酸标签的武汉-Hu-1，杆状病毒重组体（蛋白质）

货号/SKU

NR-53797

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-53797?? Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)|SARS-Related Coronavirus 2|Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus|-20°C to -80°C|Sino BiologicalAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Nucleocapsid Protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus, NR-53797.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the nucleocapsid (N) protein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724397) was produced by transfection in insect cells using a baculovirus expression system and purified. NR-53797 contains the full-length SARS-CoV-2 N protein with one mutation, G335A, and features a C-terminal poly-histidine tag. NR-53797 has a theoretical molecular weight of 47,080 daltons. Each vial contains approximately 50 ?g of purified recombinant protein in 20 mM Tris, pH 8.0, 500 mM NaCl and 10% glycerol. Note: NR-53797 was not lyophilized. Additional information and tools are available at ViPR (Virus Pathogen Resource). For more information please visit the Sino Biological website.

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的核衣壳（N）蛋白的重组形式 Coronavirus 2（SARS-COV-2），武汉 - HU-1（Genpept： YP_009724397 ）制作通过使用杆状病毒表达系统转染昆虫细胞并纯化. NR-53797包含具有一个突变，G335a的全长SARS-COV-2N蛋白，并具有C末端多组合物标签. NR-53797具有47,080道尔顿的理论分子量. 每个小瓶在20mM Tris，pH8.0,500mM NaCl和10％甘油中含有大约50μg的纯化的重组蛋白. 注意：NR-53797没有冻干. vipr （病毒病原体资源）提供了附加信息和工具. 有关更多信息，请访问 Sino Biological网站.

厂牌介绍

品牌标识

产品关键词

NR-53797 来自 SARS 相关冠状病毒 2 的核衣壳蛋白

杆状病毒重组体(蛋白质），NR-1_Vaccinia virus

手机扫描二维码阅读本页

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NR-52946来自SARS相关冠状病毒2的尖峰糖蛋白受体结合域(RBD),带有C末端组氨酸标签的武汉-Hu-1,来自HEK293T细胞(蛋白质）的重组

NR-52946_ Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293T Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-52946_ Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293T Cells(Proteins)
NR-52946 来自 SARS 相关冠状病毒 2 的尖峰糖蛋白受体结合域 (RBD)，带有 C 末端组氨酸标签的武汉-Hu-1，来自 HEK293T 细胞（蛋白质）的重组

货号/SKU

NR-52946

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

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A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

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支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-52946?? Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293T Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293T Cells| -60°C or colder|F Krammer, F Amanat, S Strohmeier, S Schultz-CherryAcknowledgment for publications should read “The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293T Cells, NR-52946.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced in transformed human embryonic kidney HEK293T cells and purified. NR-52946 lacks the signal sequence and contains 223 residues of the SARS-CoV-2 spike glycoprotein RBD and features a C-terminal hexa-histidine tag. NR-52946 has a theoretical molecular weight of 25,900 daltons. Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/ NR-52946 is intended for western blot, ELISA and animal vaccination. Each vial contains approximately 250 ?L of NR-52946 in phosphate buffered saline (PBS). Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept： QHD43416 ”在转化的人胚胎肾HEK293T细胞中生产并纯化. NR-52946缺乏信号序列并含有223个SAR-COV-2穗糖蛋白RBD的残基，并具有C末端六峰组氨酸标签. NR-52946具有理论分子量为25,900道尔顿. 注意：有关相关项的详细协议和列表，请参阅https://labs.icahn.mssm.edu/krammerlab/covid-19/ NR-52946适用于Western印迹，ELISA和动物疫苗接种. 每个小瓶在磷酸盐缓冲盐水（PBS）中含有大约250μl的NR-52946. vipr （病毒病原体资源）提供了附加信息和工具.

厂牌介绍

品牌标识

产品关键词

来自 HEK293T 细胞(蛋白质）的重组，NR-1_Vaccinia virus

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NR-52397来自SARS相关冠状病毒2的刺突糖蛋白(稳定）,带有C末端组氨酸标签的武汉-Hu-1,来自HEK293F细胞的重组(蛋白质）

NR-52397_ Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-52397_ Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells(Proteins)
NR-52397 来自 SARS 相关冠状病毒 2 的刺突糖蛋白（稳定），带有 C 末端组氨酸标签的武汉-Hu-1，来自 HEK293F 细胞的重组（蛋白质）

货号/SKU

NR-52397

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

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支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

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产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-52397?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells|-60°C or colder|F Krammer, F Amanat, S StrohmeierAcknowledgment for publications should read “The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells, NR-52397.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QJE37812) was produced by transfection of purified plasmid in human embryonic kidney HEK293F cells and purified by immobilized metal affinity chromatography. NR-52397 lacks the signal sequence and contains 1196 residues (ectodomain) of the SARS-CoV-2 spike glycoprotein; the recombinant protein was modified to remove the polybasic S1/S2 cleavage site (RRAR to A; residues 682 to 685), stabilized with a pair of mutations (K986P and V987P, wild type numbering; GenPept: YP_009724390) and includes a thrombin cleavage site, T4 foldon trimerization domain and C-terminal hexa-histidine tag. NR-52397 has a theoretical molecular weight of 137,600 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB). Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/ The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies. NR-52397 reacts with monoclonal anti-histidine tag in western blot analysis. NR-52397 is intended for western blot, ELISA and animal vaccination. Each vial of NR-52397 contains purified recombinant protein in phosphate buffered saline (PBS). Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept： QJE37812 ”是通过转染人胚胎肾HEK293F细胞中的纯化质粒并通过固定化金属亲和层析纯化. NR-52397缺乏信号序列，含有1196个残基（Ectodomain）的SARS-COV-2穗糖蛋白;修饰重组蛋白以除去多元的S1 / S2切割位点（RRAR至A;残基682至685），用一对突变稳定（K986P和V987P，野生型编号; Genpept： YP_009724390 ）并包括凝血酶切割位点，T4 Folcon三聚化结构域和C末端六核分裂标签. NR-52397具有137,600道尔顿的理论分子量.来自SARS-COV-2的三聚体S糖蛋白的晶体结构已经解决了3.46埃分辨率（PDB： 6VSB ）. 注意：有关相关项的详细协议和列表，请参阅https://labs.icahn.mssm.edu/krammerlab/covid-19/ S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S蛋白是中和抗体的靶标. NR-52397与蛋白质印迹分析中的单克隆抗组氨酸标签反应. NR-52397适用于Western Blot，ELISA和动物疫苗接种. 每个小瓶NR-52397含有磷酸盐缓冲盐水（PBS）的纯化的重组蛋白. vipr （病毒病原体资源）提供了附加信息和工具.

厂牌介绍

品牌标识

产品关键词

NR-52397 来自 SARS 相关冠状病毒 2 的刺突糖蛋白(稳定）

来自 HEK293F 细胞的重组(蛋白质），NR-1_Vaccinia virus

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NR-52366_ Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells(Proteins)

产品图片

产品英文名称

产品别名

NR-52366_ Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells(Proteins)
NR-52366 来自 SARS 相关冠状病毒 2 的刺突糖蛋白受体结合域 (RBD)，带有 C 末端组氨酸标签的武汉-Hu-1，来自 HEK293F 细胞的重组（蛋白质）

货号/SKU

NR-52366

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-52366?? Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells|-60°C or colder|F Krammer, F Amanat, S StrohmeierAcknowledgment for publications should read “The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells, NR-52366.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced by transfection of purified plasmid in human embryonic kidney HEK293F cells and purified by nickel affinity chromatography. NR-52366 lacks the signal sequence and contains 223 residues of the SARS-CoV-2 spike glycoprotein RBD and features a C-terminal hexa-histidine tag. NR-52366 has a theoretical molecular weight of 25,900 daltons. Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/ NR-52366 reacts with monoclonal anti-histidine tag in western blot analysis. NR-52366 is intended for western blot, ELISA and animal vaccination. Each vial of NR-52366 contains purified recombinant protein in phosphate buffered saline (PBS). Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept： QHD43416 ”通过在人胚胎肾HEK293F细胞中转染净化质粒并通过镍亲和层析纯化. NR-52366缺乏信号序列，并含有223个SARS-COV-2穗糖蛋白RBD的残基，并具有C末端六峰组氨酸标签. NR-52366具有理论分子量为25,900道尔顿. 注意：有关相关项的详细协议和列表，请参阅https://labs.icahn.mssm.edu/krammerlab/covid-19/ NR-52366与蛋白质印迹分析中的单克隆抗组氨酸标签反应. NR-52366适用于Western Blot，ELISA和动物疫苗接种. 每个小瓶NR-52366含有磷酸盐缓冲盐水（PBS）的纯化的重组蛋白. vipr （病毒病原体资源）提供了附加信息和工具.

厂牌介绍

品牌标识

产品关键词

来自 HEK293F 细胞的重组(蛋白质），NR-1_Vaccinia virus

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NR-52307_ Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)

产品图片

产品英文名称

产品别名

NR-52307_ Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)
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货号/SKU

NR-52307

货号/规格

库存与交货期

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人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

厂牌

BEI Resources(ATCC)

品牌