NR-53798来自SARS相关冠状病毒2的刺突糖蛋白S1结构域,带有C末端组氨酸标签的武汉-Hu-1,来自HEK293细胞的重组(蛋白质)
产品图片
货号/SKU
NR-53798
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-53798?? Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells|-20°C to -80°C|Sino BiologicalAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein S1 Domain from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293 Cells, NR-53798.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724390) was produced by transfection in human embryonic kidney HEK293 cells and purified. NR-53798 lacks the signal sequence, contains 670 residues of the SARS-CoV-2 spike glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-53798 has a theoretical molecular weight of 76,500 daltons.
The biological activity of NR-53798 was measured by its binding ability in a functional ELISA, in which immobilized human ACE2 protein (Fc tag) (Sino Biological 10108-H05H) at 2 ?g/mL (100 ?L/well) can bind NR-53798; the half maximal effective concentration (EC50) of NR-53798 is 200 to 600 ng/mL. The biological activity of NR-53798 was also measured by its binding ability using biosensor analysis, in which human ACE2 protein (Fc tag) (Sino Biological 10108-H05H) can bind NR-53798; the affinity constant is 47.7 nM.
Each vial contains approximately 50 ?g of purified recombinant protein in phosphate buffered saline (PBS, pH 7.4). Note: NR-53798 was not lyophilized.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
For more information please visit the Sino Biological website.
A recombinant form of the spike glycoprotein S1 domain from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: YP_009724390) was produced by transfection in human embryonic kidney HEK293 cells and purified. NR-53798 lacks the signal sequence, contains 670 residues of the SARS-CoV-2 spike glycoprotein (amino acid residues V16 to R685) and features a C-terminal poly-histidine tag. NR-53798 has a theoretical molecular weight of 76,500 daltons.
The biological activity of NR-53798 was measured by its binding ability in a functional ELISA, in which immobilized human ACE2 protein (Fc tag) (Sino Biological 10108-H05H) at 2 ?g/mL (100 ?L/well) can bind NR-53798; the half maximal effective concentration (EC50) of NR-53798 is 200 to 600 ng/mL. The biological activity of NR-53798 was also measured by its binding ability using biosensor analysis, in which human ACE2 protein (Fc tag) (Sino Biological 10108-H05H) can bind NR-53798; the affinity constant is 47.7 nM.
Each vial contains approximately 50 ?g of purified recombinant protein in phosphate buffered saline (PBS, pH 7.4). Note: NR-53798 was not lyophilized.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
For more information please visit the Sino Biological website.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2(SARS-COV-2),武汉-U-1(Genpept: YP_009724390 ”并纯化. NR-53798缺乏信号序列,含有670个残基的SARS-COV-2穗糖蛋白(氨基酸残基V16至R685),并具有C末端多组氨酸标签. NR-53798具有76,500道尔顿的理论分子量.
NR-53798的生物活性通过其官能团中的结合能力测量,其中固定化的人ACE2蛋白(Fc标签)(SINO生物10108-H05H)在2μg/ mL(100μl/孔)中可以结合NR- 53798; NR-53798的半最大有效浓度(EC 50 )为200至600
ng / ml. NR-53798的生物活性也通过其使用生物传感器分析的结合能力来测量,其中人ACE2蛋白(FC标签)(SINO生物10108-H05H)可以结合NR-53798;亲和力常数为47.7 nm.
每个小瓶在磷酸盐缓冲盐水中含有大约50μg的纯化的重组蛋白(PBS,pH7.4). 注意:NR-53798没有冻干.
vipr (病毒病原体资源)提供了附加信息和工具.
有关更多信息,请访问 Sino Biological网站.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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