NR-52397来自SARS相关冠状病毒2的刺突糖蛋白(稳定）,带有C末端组氨酸标签的武汉-Hu-1,来自HEK293F细胞的重组(蛋白质）
产品图片
货号/SKU
NR-52397
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-52397?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells|-60°C or colder|F Krammer, F Amanat, S StrohmeierAcknowledgment for publications should read “The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from HEK293F Cells, NR-52397.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QJE37812) was produced by transfection of purified plasmid in human embryonic kidney HEK293F cells and purified by immobilized metal affinity chromatography. NR-52397 lacks the signal sequence and contains 1196 residues (ectodomain) of the SARS-CoV-2 spike glycoprotein; the recombinant protein was modified to remove the polybasic S1/S2 cleavage site (RRAR to A; residues 682 to 685), stabilized with a pair of mutations (K986P and V987P, wild type numbering; GenPept: YP_009724390) and includes a thrombin cleavage site, T4 foldon trimerization domain and C-terminal hexa-histidine tag. NR-52397 has a theoretical molecular weight of 137,600 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
NR-52397 reacts with monoclonal anti-histidine tag in western blot analysis. NR-52397 is intended for western blot, ELISA and animal vaccination.
Each vial of NR-52397 contains purified recombinant protein in phosphate buffered saline (PBS).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QJE37812) was produced by transfection of purified plasmid in human embryonic kidney HEK293F cells and purified by immobilized metal affinity chromatography. NR-52397 lacks the signal sequence and contains 1196 residues (ectodomain) of the SARS-CoV-2 spike glycoprotein; the recombinant protein was modified to remove the polybasic S1/S2 cleavage site (RRAR to A; residues 682 to 685), stabilized with a pair of mutations (K986P and V987P, wild type numbering; GenPept: YP_009724390) and includes a thrombin cleavage site, T4 foldon trimerization domain and C-terminal hexa-histidine tag. NR-52397 has a theoretical molecular weight of 137,600 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
NR-52397 reacts with monoclonal anti-histidine tag in western blot analysis. NR-52397 is intended for western blot, ELISA and animal vaccination.
Each vial of NR-52397 contains purified recombinant protein in phosphate buffered saline (PBS).
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept： QJE37812 ”是通过转染人胚胎肾HEK293F细胞中的纯化质粒并通过固定化金属亲和层析纯化. NR-52397缺乏信号序列，含有1196个残基（Ectodomain）的SARS-COV-2穗糖蛋白;修饰重组蛋白以除去多元的S1 / S2切割位点（RRAR至A;残基682至685），用一对突变稳定（K986P和V987P，野生型编号; Genpept： YP_009724390 ）并包括凝血酶切割位点，T4 Folcon三聚化结构域和C末端六核分裂标签. NR-52397具有137,600道尔顿的理论分子量.来自SARS-COV-2的三聚体S糖蛋白的晶体结构已经解决了3.46埃分辨率（PDB： 6VSB ）.
注意：有关相关项的详细协议和列表，请参阅https://labs.icahn.mssm.edu/krammerlab/covid-19/
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S蛋白是中和抗体的靶标.
NR-52397与蛋白质印迹分析中的单克隆抗组氨酸标签反应. NR-52397适用于Western Blot，ELISA和动物疫苗接种.
每个小瓶NR-52397含有磷酸盐缓冲盐水（PBS）的纯化的重组蛋白.
vipr （病毒病原体资源）提供了附加信息和工具.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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