产品描述信息
NR-722??SARS coronavirus, Spike (S) Protein deltaTM, Recombinant from Baculovirus (Proteins)|SARS coronavirus|Spike (S) Protein deltaTM, Recombinant from Baculovirus |-20°C or colder|NIH Biodefense and Emerging Infections Research Resources RepositoryAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-CoV Spike (S) Protein deltaTM, Recombinant from Baculovirus, NR-722."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-722 is a truncated, glycosylated recombinant form of the SARS-CoV spike (S) external envelope glycoprotein. A 60-amino acid deletion of the transmembrane domain (deltaTM) was made to the C-terminal end of the protein. A baculovirus signal sequence, MPLYKLLNVLWLVAVSNA, was added to the N-terminal end of the protein to enhance expression. The SARS-CoV spike (S) protein deltaTM was produced in Sf9 insect cells using a baculovirus expression vector system and was purified using conventional chromatographic techniques. The baculovirus signal sequence is efficiently removed during translocation of the protein across the membrane. The resulting protein, NR-722, has a molecular weight of approximately 150,000 daltons. The purified protein was demonstrated to be functionally active based on its ability to bind to a recombinant form of its functional receptor, angiotensin I converting enzyme-2.Each vial contains approximately 1 mg of NR-722 in 2.5 mM sodium phosphate (pH 7.2) and 150 mM NaCl.