产品描述信息
NR-31213?? Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cell Wall Fraction(Antigen Preparations)|Mycobacterium bovis|Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cell Wall Fraction|-80°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium bovis, Strain AF 2122/97 (ATCC ? BAA-935?), Cell Wall Fraction, NR-31213."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-31213 is a preparation of the cell wall fraction of Mycobacterium bovis, strain AF 2122/97 (ATCC ? BAA-935?), and contains proteins and non-protein compounds such as mAGP.The culture was grown to late-log phase in sodium pyruvate alanine salts medium, washed with PBS pH 7.4, and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation at 27,000 x g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate, quantified by BCA protein assay for protein content, and frozen. Each vial contains approximately 1 mg of protein in 10 mM ammonium bicarbonate.