NR-31215_牛分枝杆菌,菌株AF2122/97(ATCC?BAA-935?),细胞溶胶组分(抗原制剂）
产品图片
货号/SKU
NR-31215
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-31215?? Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cytosol Fraction(Antigen Preparations)|Mycobacterium bovis|Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cytosol Fraction|-80°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium bovis, Strain AF 2122/97 (ATCC ? BAA-935?), Cytosol Fraction, NR-31215."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-31215 is a preparation of the cytosol fraction of Mycobacterium bovis, strain AF 2122/97 (ATCC ? BAA-935?), and contains cytosolic proteins and soluble material released from the cell wall during disruption of the bacilli.
The culture was grown to late-log phase in sodium pyruvate alanine salts medium, washed with PBS and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation (27,000 x g). The supernatant was then subjected to centrifugation (100,000 x g) for four hours, collected and dialyzed against 10 mM ammonium bicarbonate. The protein content was determined using the BCA protein assay.
Each vial contains approximately 1 mg of protein in 10 mM ammonium bicarbonate.
NR-31215 is a preparation of the cytosol fraction of Mycobacterium bovis, strain AF 2122/97 (ATCC ? BAA-935?), and contains cytosolic proteins and soluble material released from the cell wall during disruption of the bacilli.
The culture was grown to late-log phase in sodium pyruvate alanine salts medium, washed with PBS and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation (27,000 x g). The supernatant was then subjected to centrifugation (100,000 x g) for four hours, collected and dialyzed against 10 mM ammonium bicarbonate. The protein content was determined using the BCA protein assay.
Each vial contains approximately 1 mg of protein in 10 mM ammonium bicarbonate.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将被发送到发货前的批准. NR-31215是一种制备Cytosol分数的分枝杆菌Bovis，菌株AF 2122/97 （ATCC ? baa-935?），并含有在杆菌中破坏中从细胞壁释放的细胞溶胶蛋白和可溶物. 文化生长至后勤阶段丙酮酸钠丙氨酸盐培养基，用PBS洗涤并通过γ辐射灭活.将溶液在含有8mM EDTA，DNA酶，RNA酶和蛋白酶抑制剂片中的PBS中浓度的2g / ml浓度悬浮，并在4℃下在法国压力下破裂.通过低速（3,000×g）离心除去不间断的细胞.通过离心（27,000×g）分离细胞壁（27,000×g）.然后将上清液用于离心（100,000×g）4小时，收集并透析10mM碳酸氢铵.使用BCA蛋白质测定法测定蛋白质含量. 每个小瓶在10mM碳酸氢铵中含有约1mg蛋白质. sp6
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