细胞壁组分(抗原制剂），NR-1_Vaccinia virus

NR-31213?? Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cell Wall Fraction(Antigen Preparations)|Mycobacterium bovis|Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cell Wall Fraction|-80°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium bovis, Strain AF 2122/97 (ATCC ^? BAA-935?), Cell Wall Fraction, NR-31213."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-31213 is a preparation of the cell wall fraction of Mycobacterium bovis, strain AF 2122/97 (ATCC ^? BAA-935?), and contains proteins and non-protein compounds such as mAGP.The culture was grown to late-log phase in sodium pyruvate alanine salts medium, washed with PBS pH 7.4, and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation at 27,000 x g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate, quantified by BCA protein assay for protein content, and frozen. Each vial contains approximately 1 mg of protein in 10 mM ammonium bicarbonate.

NR-14830?? Mycobacterium tuberculosis, Strain HN878, Cell Wall Fraction(Antigen Preparations)|Mycobacterium tuberculosis|Mycobacterium tuberculosis, Strain HN878, Cell Wall Fraction|-80°C or colder|BEI Resources or NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain HN878, Cell Wall Fraction, NR-14830."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-14830 is a preparation of the cell wall fraction of Mycobacterium tuberculosis (M. tuberculosis), strain HN878, and contains proteins and non-protein compounds such as mAGP. The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS pH 7.4, and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation at 27,000 x g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate. The protein content was determined using the BCA protein assay. Each vial of NR-14830 contains approximately 1 mg of cell wall fraction from M. tuberculosis, strain HN878 provided in 10 mM ammonium bicarbonate.

NR-14828?? Mycobacterium tuberculosis, Strain H37Rv, Cell Wall Fraction(Antigen Preparations)|Mycobacterium tuberculosis|Mycobacterium tuberculosis, Strain H37Rv, Cell Wall Fraction|-80°C or colder|BEI Resources or NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Cell Wall Fraction, NR-14828."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-14828 is a preparation of the cell wall fraction of Mycobacterium tuberculosis, strain H37Rv and contains proteins and non-protein compounds such as mAGP.The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS pH 7.4, and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation at 27,000 x g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate, quantified by BCA protein assay for protein content, and frozen.Each vial contains approximately 1 mg of NR-14828 provided as a frozen pellet.Note: NR-14828 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used. Cell wall fractions may not fully solubilize, but a uniform suspension can be achieved with bath sonification.