产品描述信息
NR-14831?? Mycobacterium tuberculosis, Strain H37Rv, Cell Membrane Fraction(Antigen Preparations)|Mycobacterium tuberculosis|Mycobacterium tuberculosis, Strain H37Rv, Cell Membrane Fraction|-80°C or colder|BEI Resources or NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Cell Membrane Fraction, NR-14831."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-14831 is a preparation of the cell membrane fraction of Mycobacterium tuberculosis, strain H37Rv, and contains the cytoplasmic membrane and components of the outer lipid layer. The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation at 27,000 x g. The supernatant was subjected to a 100,000 x g centrifugation for four hours. The resulting membrane pellet was washed with PBS, then suspended and dialyzed in 0.01 M ammonium bicarbonate. Protein content was determined using the BCA protein assay.Each vial or NR-14831 contains approximately 1 mg of cell membrane fraction from M. tuberculosis, strain H37Rv in 10 mM ammonium bicarbonate.