NR-44100_牛分枝杆菌,菌株AF2122/97(ATCC?BAA-935?),总脂质(抗原制剂）
产品图片
货号/SKU
NR-44100
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-44100?? Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Total Lipids (Antigen Preparations)|Mycobacterium bovis|Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Total Lipids |Room temperature (dry atmosphere)|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium bovis, Strain AF 2122/97 (ATCC ? BAA-935?), Total Lipids, NR-44100."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-44100 is a preparation of the total cellular lipids of Mycobacterium bovis (M. bovis), strain AF 2122/97 (ATCC ? BAA-935?), including those with known biological activities, such as trehalose dimycolate (TDM) and sulpholipids. The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS, inactivated by gamma irradiation and dried. The cellular lipids were extracted with 30 mL of chloroform/methanol (2:1) per gram of cells at 55°C for 18 hours. Cells were removed by filtration and contaminating hydrophilic molecules were removed by biphasic partitioning with water (Folch Wash). The organic phase of the Folch wash was collected and dried.
Each vial contains approximately 5 mg of dried total lipids from M. bovis, strain AF 2122/97 (ATCC ? BAA-935?).
Note: Total lipid is soluble in chloroform:methanol (2:1). DMSO can also be used depending on the downstream application.
NR-44100 is a preparation of the total cellular lipids of Mycobacterium bovis (M. bovis), strain AF 2122/97 (ATCC ? BAA-935?), including those with known biological activities, such as trehalose dimycolate (TDM) and sulpholipids. The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS, inactivated by gamma irradiation and dried. The cellular lipids were extracted with 30 mL of chloroform/methanol (2:1) per gram of cells at 55°C for 18 hours. Cells were removed by filtration and contaminating hydrophilic molecules were removed by biphasic partitioning with water (Folch Wash). The organic phase of the Folch wash was collected and dried.
Each vial contains approximately 5 mg of dried total lipids from M. bovis, strain AF 2122/97 (ATCC ? BAA-935?).
Note: Total lipid is soluble in chloroform:methanol (2:1). DMSO can also be used depending on the downstream application.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到NIAID进行批准. NR-44100是NR-44100的制备，对分枝杆菌（BOVIS）的总细胞脂质（BOVIS） ，菌株AF 2122/97（ATCC ? BAA-935?），包括具有已知生物活性的那些，例如海藻糖二氯化物（TDM）和磺基.将培养物生长在甘油 - 丙氨酸 - 盐培养基中的后对数，用PBS洗涤，通过γ辐射灭活并干燥.在55℃下每克细胞用30ml氯仿/甲醇（2：1）萃取细胞脂质18小时.通过过滤除去细胞并通过双相分配除去污染亲水分子（FolCH洗涤）.收集叶片洗涤的有机相并干燥. 每个小瓶含有约5mg干燥的总脂质，从 m. BOVIS ，菌株AF 2122/97（ATCC ? BAA-935?）. 注意：总脂质可溶于氯仿：甲醇（2：1）. DMSO也可以根据下游应用程序使用.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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