NR-31213_牛分枝杆菌,菌株AF2122/97(ATCC?BAA-935?),细胞壁组分(抗原制剂）
产品图片
货号/SKU
NR-31213
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-31213?? Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cell Wall Fraction(Antigen Preparations)|Mycobacterium bovis|Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cell Wall Fraction|-80°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium bovis, Strain AF 2122/97 (ATCC ? BAA-935?), Cell Wall Fraction, NR-31213."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-31213 is a preparation of the cell wall fraction of Mycobacterium bovis, strain AF 2122/97 (ATCC ? BAA-935?), and contains proteins and non-protein compounds such as mAGP.
The culture was grown to late-log phase in sodium pyruvate alanine salts medium, washed with PBS pH 7.4, and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation at 27,000 x g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate, quantified by BCA protein assay for protein content, and frozen.
Each vial contains approximately 1 mg of protein in 10 mM ammonium bicarbonate.
NR-31213 is a preparation of the cell wall fraction of Mycobacterium bovis, strain AF 2122/97 (ATCC ? BAA-935?), and contains proteins and non-protein compounds such as mAGP.
The culture was grown to late-log phase in sodium pyruvate alanine salts medium, washed with PBS pH 7.4, and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation at 27,000 x g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate, quantified by BCA protein assay for protein content, and frozen.
Each vial contains approximately 1 mg of protein in 10 mM ammonium bicarbonate.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. NR-31213是一种制备分枝杆菌BOVIS的细胞壁分数，菌株AF 2122 / 97（ATCC ? baa-935?），并含有蛋白质和非蛋白质化合物，如magp. 培养物生长至丙酮酸钠丙氨酸盐培养基中的后对数相，用PBS pH 7.4洗涤，并通过γ辐射灭活.将溶液在含有8mM EDTA，DNA酶，RNA酶和蛋白酶抑制剂片中的PBS中浓度的2g / ml浓度悬浮，并在4℃下在法国压力下破裂.通过低速（3,000×g）离心除去不间断的细胞.通过以27,000×g离心1小时，将细胞壁分离一小时并在PBS中洗涤两次.将最终的细胞壁沉淀悬浮并在10mM碳酸氢铵中透析并透析，通过BCA蛋白质测定来定量蛋白质含量和冷冻. 每个小瓶在10mM碳酸氢盐中含有约1mg蛋白质.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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