产品描述信息
NR-31215?? Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cytosol Fraction(Antigen Preparations)|Mycobacterium bovis|Mycobacterium bovis, Strain AF 2122/97 (ATCC? BAA-935?), Cytosol Fraction|-80°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium bovis, Strain AF 2122/97 (ATCC ? BAA-935?), Cytosol Fraction, NR-31215."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-31215 is a preparation of the cytosol fraction of Mycobacterium bovis, strain AF 2122/97 (ATCC ? BAA-935?), and contains cytosolic proteins and soluble material released from the cell wall during disruption of the bacilli.The culture was grown to late-log phase in sodium pyruvate alanine salts medium, washed with PBS and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation (27,000 x g). The supernatant was then subjected to centrifugation (100,000 x g) for four hours, collected and dialyzed against 10 mM ammonium bicarbonate. The protein content was determined using the BCA protein assay.Each vial contains approximately 1 mg of protein in 10 mM ammonium bicarbonate.