磁微粒化学发光用抗原抗体

MW: 28,000
Extinction Coefficient: 1.22

Frozen in 20 mM Na Acetate, pH 4.5, with 1 mM EDTA and 100mM NaCl

Storage:

A more basic protein than Cathepsin B or L. Functions as both an aminopeptidase and endopeptidase. Cathepsin H, like cathepsin L and B, is involved in the catabolism of proteins in the lysosomal system.

Activity: > 1 unit/mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of L-arginine-beta-napthylamide per minute at 40oC using 75 mM potasium phosphate, pH 6.8, with 1 mM EDTA and 3 mM cysteine as the activation buffer.

Prepared from tissue shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.

Product Citation:
Douglas Lu, Weiya, Lydiane Funkelstein, Thomas Toneff, Thomas Reinheckel, Christoph Peters, and Vivian Hook. "Cathepsin H functions as an aminopeptidase in secretory vesicles for production of enkephalin and galanin peptide neurotransmitters."?Journal of neurochemistry?122, no. 3 (2012): 512-522.

Ref:
Barrett, A.J. and Kirschke, H. 1981. Methods Enzymol. 80, 535.
Barrett, A.J. 1977. Proteinases in Mammalian Cells and Tissues. (A. Barrett, ed.) 4, 209

MW: 23,500
Extinction Coefficient: 0.664

Salt-free lyophilized solid.

Storage: -20°C

Buy Purified Native Human Cathepsin G (CatG), Human Neutrophil.
Bulk Qty Available.

This enzyme degrades collagen and proteoglycan and can also cause the release of angiotensin II from angiotensin I or angiotensinogen. It has been implicated in connective tissue diseases such as emphysema and rheumatoid arthritis.

Ref: Travis, J. et al. 1978. Biochemistry. 17, 5651.

Activity: 2-4 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of Suc-Ala-Ala Pro-Phe-pNA (1 mM) per minute at 25oC in 160 mM Tris-HCl, pH 7.4, with 1.6 M NaCl.

Purity: >=95% by SDS-PAGE

Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti- HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.

Product Citations:
Mittendorf, Elizabeth A., Gheath Alatrash, Na Qiao, Yun Wu, Pariya Sukhumalchandra, Lisa S. St John, Anne V. Philips et al. "Breast cancer cell uptake of the inflammatory mediator neutrophil elastase triggers an anticancer adaptive immune response."?Cancer research?72, no. 13 (2012): 3153-3162.

Baumann, Mathias, Christine TN Pham, and Charaf Benarafa. "SerpinB1 is critical for neutrophil survival through cell-autonomous inhibition of cathepsin G."?Blood?121, no. 19 (2013): 3900-3907
van den Berg, Carmen W., et al. "Mechanism of Neutrophil Dysfunction: Neutrophil Serine Proteases Cleave and Inactivate the C5a Receptor."?The Journal of Immunology?192.4 (2014): 1787-1795.

Towstyka NY, Shiromizu CM, Keitelman I, Sabbione F, et al.
Modulation of γδ T cell activation by neutrophil elastase.
Immunology. 2017 Sep 9. doi: 10.1111/imm.12835.

Ksiazek M, et al.
Miropin, a Novel Bacterial Serpin from the Periodontopathogen Tannerella forsythia, Inhibits a Broad Range of Proteases by Using Different Peptide Bonds within the Reactive Center Loop.
J Biol Chem. 2015 Jan 2;290(1):658-70. doi: 10.1074/jbc.M114.601716. Epub 2014 Nov 11.

Thomas MP, et al.
Leukocyte Protease Binding to Nucleic Acids Promotes Nuclear Localization and Cleavage of Nucleic Acid Binding Proteins.
J Immunol. 2014 Jun 1;192(11):5390-7. doi: 10.4049/jimmunol.1303296. Epub 2014 Apr 25.

MW: 42,000
Extinction Coefficient: 1.0

Lyophilized from 2 mM Na phosphate, pH 6.5.

Storage:

Buy Purified Native Human Cathepsin D (CatD), Human Liver.
Bulk Qty Available.

Cathepsin D is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node-negative and node-positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimer's plaques. As cathepsin D activity is increased by cigarette smoke, the enzyme may contribute to lung tissue damage in smokers.

Activity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A 280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, pH 1.8 (0.2% in reaction mixture). Buffer: 100 mM formate, pH 3.3.

Purity: >=95% by SDS-PAGE

Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Product Citations:
Armstrong, Andrea, Niklas Mattsson, Hanna Appelqvist, Camilla Janefjord, Linnea Sandin, Lotta Agholme, Bob Olsson et al. "Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer’s Disease."?Neuromolecular medicine?(2013): 1-11
Appelqvist, Hanna, et al. "Lysosome-mediated apoptosis is associated with cathepsin D-specific processing of bid at Phe24, Trp48, and Phe183."Annals of Clinical & Laboratory Science?42.3 (2012): 231-242.

References:
Barrett, A.J. 1977. Proteinases in Mammalian Cells and Tissues. (A. Barrett, ed.) 4, 209.
Barrett, A.J. 1970. Biochemistry. 117, 601.
Tandon, A.K. et al. 1990. N. Engl. J. Med. 322, 297.
Cataldo, A.M. et al. 1990. Brain Res. 513, 181.
Chang, J.C. et al. Am. Rev. Respir. Dis. 140, 958.

MW: 42,000
Extinction Coefficient: 1.0

Lyophilized from 2 mM Na phosphate, pH 6.5.

Storage:

Buy Purified Native Human Cathepsin D (CatD), Human Liver.
Bulk Qty Available.

Cathepsin D is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node-negative and node-positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimer's plaques. As cathepsin D activity is increased by cigarette smoke, the enzyme may contribute to lung tissue damage in smokers.

Activity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A 280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, pH 1.8 (0.2% in reaction mixture). Buffer: 100 mM formate, pH 3.3.

Purity: >=95% by SDS-PAGE

Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Product Citations:
Armstrong, Andrea, Niklas Mattsson, Hanna Appelqvist, Camilla Janefjord, Linnea Sandin, Lotta Agholme, Bob Olsson et al. "Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer’s Disease."?Neuromolecular medicine?(2013): 1-11
Appelqvist, Hanna, et al. "Lysosome-mediated apoptosis is associated with cathepsin D-specific processing of bid at Phe24, Trp48, and Phe183."Annals of Clinical & Laboratory Science?42.3 (2012): 231-242.

References:
Barrett, A.J. 1977. Proteinases in Mammalian Cells and Tissues. (A. Barrett, ed.) 4, 209.
Barrett, A.J. 1970. Biochemistry. 117, 601.
Tandon, A.K. et al. 1990. N. Engl. J. Med. 322, 297.
Cataldo, A.M. et al. 1990. Brain Res. 513, 181.
Chang, J.C. et al. Am. Rev. Respir. Dis. 140, 958.

MW: 42,000
Extinction Coefficient: 1.0

Lyophilized from 2 mM Na phosphate, pH 6.5.

Storage:

Buy Purified Native Human Cathepsin D (CatD), Human Liver.
Bulk Qty Available.

Cathepsin D is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node-negative and node-positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimer's plaques. As cathepsin D activity is increased by cigarette smoke, the enzyme may contribute to lung tissue damage in smokers.

Activity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A 280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, pH 1.8 (0.2% in reaction mixture). Buffer: 100 mM formate, pH 3.3.

Purity: >=95% by SDS-PAGE

Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Product Citations:
Armstrong, Andrea, Niklas Mattsson, Hanna Appelqvist, Camilla Janefjord, Linnea Sandin, Lotta Agholme, Bob Olsson et al. "Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer’s Disease."?Neuromolecular medicine?(2013): 1-11
Appelqvist, Hanna, et al. "Lysosome-mediated apoptosis is associated with cathepsin D-specific processing of bid at Phe24, Trp48, and Phe183."Annals of Clinical & Laboratory Science?42.3 (2012): 231-242.

References:
Barrett, A.J. 1977. Proteinases in Mammalian Cells and Tissues. (A. Barrett, ed.) 4, 209.
Barrett, A.J. 1970. Biochemistry. 117, 601.
Tandon, A.K. et al. 1990. N. Engl. J. Med. 322, 297.
Cataldo, A.M. et al. 1990. Brain Res. 513, 181.
Chang, J.C. et al. Am. Rev. Respir. Dis. 140, 958.

MW: 27,500
Extinction Coefficient: 2.0

Frozen in 50 mM Na Acetate, pH 5.0, with 1 mM EDTA

Storage:

Buy Purified Native Human Cathepsin B (CatB), Human Liver.
Bulk qty. available.

Cathepsin B, a cysteine proteinase, is located in lysosomes and is involved in tissue degradation and restructuring. Specifically, cathepsin B is believed to be involved in the intracellular digestion of extracellular proteins taken up by endocytosis.

Ref: Rich D.H., Brown M.A. and Barrett A.J. 1986 Biochem J, 235, 731-734.

Activity: >= 200 units/mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of Z-Arg-Arg-beta-naphthylamide per minute at 40oC, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.

Purity: >=95% by SDS-PAGE

Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Product Citations:
Armstrong, Andrea, Niklas Mattsson, Hanna Appelqvist, Camilla Janefjord, Linnea Sandin, Lotta Agholme, Bob Olsson et al. "Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer’s Disease."Neuromolecular medicine?16, no. 1 (2014): 150-160.
Watson, Andre, Cara Yocum, Vaibhav Pandit, and Shiva P. Kotha. "Optimizing Polymeric Nanoparticle Core Designs for Gene Delivery." In?Bioengineering Conference (NEBEC), 2013 39th Annual Northeast, pp. 1-2. IEEE, 2013.
Toneff, Thomas, Lydiane Funkelstein, Charles Mosier, Armen Abagyan, Michael Ziegler, and Vivian Hook. "Beta-Amyloid Peptides Undergo Regulated Co-Secretion with Neuropeptide and Catecholamine Neurotransmitters."Peptides?(2013).
Elie, Benelita Tina, Vasilena Gocheva, Tanaya Shree, Stacie A. Dalrymple, Leslie J. Holsinger, and Johanna A. Joyce. "Identification and pre-clinical testing of a reversible cathepsin protease inhibitor reveals anti-tumor efficacy in a pancreatic cancer model."?Biochimie?92, no. 11 (2010): 1618-1624.

MW: ~60kDa subunit. Tetrameric protein

Extinction Coefficient: 63955 M-1 cm-1, at 280 nm measured in water.

Lyophilized

Storage: -20°C or lower

Applications (Research & Life Sciences, Diagnostics & Clinical Chemistry, Industry)
-Free radicals & effects of oxidative stress in gene expression and apoptosis.
-DNA repair systems.
-Aging research.
-As a clean-up system to decompose residual hydrogen peroxide produced by different enzymatic reactions (i.e. amino acid oxidases).
-Control and standard in testing/assay validation.
-Positive control in catalase detection kits.
-For wastewater treatment of hydrogen peroxide contaminated water.
-Gluconic and glycolic acid production.
-And others.

Activity: >=8000 U/mg protein

Source: Escherichia coli (recombinant enzyme from psychrotolerant bacterium).

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

MW: ~60kDa subunit. Tetrameric protein

Extinction Coefficient: 63955 M-1 cm-1, at 280 nm measured in water.

Lyophilized

Storage: -20°C or lower

Applications (Research & Life Sciences, Diagnostics & Clinical Chemistry, Industry)
-Free radicals & effects of oxidative stress in gene expression and apoptosis.
-DNA repair systems.
-Aging research.
-As a clean-up system to decompose residual hydrogen peroxide produced by different enzymatic reactions (i.e. amino acid oxidases).
-Control and standard in testing/assay validation.
-Positive control in catalase detection kits.
-For wastewater treatment of hydrogen peroxide contaminated water.
-Gluconic and glycolic acid production.
-And others.

Activity: >=8000 U/mg protein

Source: Escherichia coli (recombinant enzyme from psychrotolerant bacterium).

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

MW: 230,000
Extinction Coefficient: 1.39

Frozen in 50 mM Tris-HCl, pH 8.0.

Storage:

Bulk Qty Available.

Composed of four identical subunits each with a molecular weight of 58,000. Catalyzes the reaction 2H2O2 to H2O+O2. Catalase, along with the superoxides dismutase and glutathione peroxidase, controls the levels of oxygen-derived free radicals in mammalian cells, and together may function as a somatic oxidant defense.

Ref. Bonaventura, J. et Al. 1972. Arch. Biochem. Biophys. 150, 606; Maehly, A.C. and Chance, B. 1954. Methods Biochem. Anal. 1,357.

Purity: >=95% by SDS-PAGE

Activity: >50,000 units/mg protein. One unit is equal to the amount of enzyme which will decompose one micromole of hydrogen peroxide per minute at 25 degrees Celcius, pH 7.0.

Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Product Citation:
Xiao, Wusheng, Yueming Zhu, Ehab H. Sarsour, Amanda L. Kalen, Nukhet Aykin-Burns, Douglas R. Spitz, and Prabhat C. Goswami. "Selenoprotein P regulates 1-(4-Chlorophenyl)-benzo-2, 5-quinone induced oxidative stress and toxicity in human keratinocytes."?Free Radical Biology and Medicine?(2013)

MW: 230,000
Extinction Coefficient: 1.39

Frozen in 50 mM Tris-HCl, pH 8.0.

Storage:

Bulk Qty Available.

Composed of four identical subunits each with a molecular weight of 58,000. Catalyzes the reaction 2H2O2 to H2O+O2. Catalase, along with the superoxides dismutase and glutathione peroxidase, controls the levels of oxygen-derived free radicals in mammalian cells, and together may function as a somatic oxidant defense.

Ref. Bonaventura, J. et Al. 1972. Arch. Biochem. Biophys. 150, 606; Maehly, A.C. and Chance, B. 1954. Methods Biochem. Anal. 1,357.

Purity: >=95% by SDS-PAGE

Activity: >50,000 units/mg protein. One unit is equal to the amount of enzyme which will decompose one micromole of hydrogen peroxide per minute at 25 degrees Celcius, pH 7.0.

Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Product Citation:
Xiao, Wusheng, Yueming Zhu, Ehab H. Sarsour, Amanda L. Kalen, Nukhet Aykin-Burns, Douglas R. Spitz, and Prabhat C. Goswami. "Selenoprotein P regulates 1-(4-Chlorophenyl)-benzo-2, 5-quinone induced oxidative stress and toxicity in human keratinocytes."?Free Radical Biology and Medicine?(2013)

MW: 110,000

20 mM imidazole-HCl, 5mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.

Storage: -70?C or lower; avoid freeze/thaw cycles

Activity: >= 1000 units/mg protein prior to freezing.
One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 minute at 25°C using the Calpain Activity Assay Kit, Fluorogenic (EMD Cat. No, QIA120). Note 1 caseinolytic unit=9 fluorogenic units.

Prepared from porcine erythrocytes obtained from a USDA certified collection center.

Country of Origin: Porcine erythrocyte was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.