磁微粒化学发光用抗原抗体

Species Reactivity: Human

Host: Rabbit

Immunogen Source: Human Liver

Form: Ig Fraction (Standard Antisera) obtained by ammonium sulfate precipitation.

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Protein concentration: 21.43 mg/mL

Working Dilution: >1:512 by indirect ELISA

Western Blot: At a dilution of 1:8000 the antibody reacts with 0.06 and 0.15 ug of purified human liver cathepsin H and does not react with cathepsins B, D or L.

Not suitable for IEP.

The Cathepsin H immunogen used to prepare this antibody was purified in the Lab laboratory. It is a native protein, whole molecule, isolated from human liver.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

Species Reactivity: Human Liver

Host: Rabbit

Immunogen Source: Human Liver

Form: Ig Fraction (Standard Antisera) obtained by ammonium sulfate precipitation.

Applications: ELISA, WB (not suitable for IEP)

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Protein Concentration: 20.6 mg/mL

Working Dilution: >1:16000 by indirect ELISA.

Western Blot: At a dilution of 1:4000 the antibody reacts with 0.075, 0.15, and 0.3 ug human liver cathepsin D and does not react with cathepsins B, H or L.

The Cathepsin D immunogen used to prepare this antibody was purified in the Lab laboratory. It is a native protein, whole molecule, isolated from human liver.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

References:
W?ster P, Rosdahl I, ?llinger K. Cell fate regulated by nuclear factor-κB- and activator protein-1-dependent signalling in human melanocytes exposed to ultraviolet A and ultraviolet B. Br J Dermatol. 2014 Dec;171(6):1336-46. doi: 10.1111/bjd.13278. Epub 2014 Nov 14.

W?ster P, Eriksson I, Vainikka L, Rosdahl I, ?llinger K. Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation. Sci Rep. 2016 Jun 13;6:27890. doi: 10.1038/srep27890.

Species Reactivity: Human

Host: Rabbit

Immunogen Source: Human Liver

Form: Ig Fraction (Standard Antisera) obtained by ammonium sulfate precipitation.

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Protein Concentration: 4.24 mg/mL

Working Dilution: 1:1024 by indirect ELISA using purified antigen

Western Blot: At a dilution of 1:4000, the antibody reacts with 0.3 and 0.6 ug of purified human liver cathepsin B and does not react with 0.3 ug purified human liver cathepsins D, H or L.

The Cathepsin B immunogen used to prepare this antibody was purified in the Lab laboratory. It is a native protein, whole molecule, isolated from human liver.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

Species Reactivity: Human

Host: Rabbit

Immunogen Source: Human Erythrocyte

Form: IgG fraction from antisera obtained by ammonium sulfate precipitation and DEAE-Sephacel ion-exchange chromatography.

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Working Dilution: >1:2000 by indirect ELISA using ALPH-conjugated secondary antibody

Western Blot: At 1:16000 dilution, antisera reacts to 0.75, 0.15, and 0.3 ug catalase and to 1 uL of red blood cell lysate using ALPH conjugated secondary antibody.

IEP: Single arc upon immunoelectrophoresis of red blood cell lysate.

The Catalase immunogen used to prepare this antibody was purified in the Lab laboratory. It is a native protein, whole molecule, isolated from human erythrocytes.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

Ref:
Kim SJ, Cheresh P, Jablonski RP, Morales-Nebreda L, Cheng Y, Hogan E, Yeldandi A, Chi M, Piseaux R, Ridge K, Michael Hart C, Chandel N, Scott Budinger GR, Kamp DW. Mitochondrial catalase overexpressed transgenic mice are protected against lung fibrosis in part via preventing alveolar epithelial cell mitochondrial DNA damage. Free Radic Biol Med. 2016 Dec;101:482-490. doi: 10.1016/j.freeradbiomed.2016.11.007. Epub 2016 Nov 11.

Xiao W and Goswami PC. Down-regulation of peroxisome proliferator activated receptor γ coactivator 1α induces oxidative stress and toxicity of 1-(4-Chlorophenyl)-benzo-2,5-quinone in HaCaT human keratinocytes. Toxicol In Vitro. 2015 Oct;29(7):1332-8. doi: 10.1016/j.tiv.2015.05.009. Epub 2015 May 22.

Species Reactivity: Human

Host: Rabbit

Immunogen Source: Human Neutrophil

Form: Ig Fraction (Standard Antisera) obtained by ammonium sulfate precipitation.

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Protein Concentration: 19.4 mg/mL

Working Dilution: 1:4000 by indirect ELISA

Western Blot: At a dilution of 1:2000, the antisera reacts to 0.3 ug and 0.6 ug of antigen. Does not react to 0.5 ug of purified Lysozyme, Myeloperoxidase, Cathepsin G, Proteinase 3, or Elastase.

IEP: Single arc on IEP of human neutrophil granule extract.

The Azurocidin immunogen used to prepare this antibody was purified in the Lab laboratory. It is a native protein, whole molecule, isolated from human neutrophils.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

Species Reactivity: Human

Host: Rabbit

Immunogen Source: Human Plasma

Form: IgG fraction from antisera obtained by ammonium sulfate precipitation and DEAE-Sephacel ion-exchange chromatography.

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Protein Concentration: 2.13 mg/mL.

Working Dilution: 1:1000 by indirect ELISA.

Western Blot: Does not cross react with Apolipoproteins AI, AII, B, CI, CIII, and E. Good reaction vs. 0.3 ug Apo C2 at a dilution of 1:2500.

IEP: Single arc vs purified antigen and whole human plasma.

The Apolipoprotein CII immunogen used to prepare this antibody was purified in the Lab laboratory. It is a native protein, whole molecule, isolated from human plasma.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

Species Reactivity: Human

Host: Rabbit

Form: IgG fraction from antisera obtained by ammonium sulfate precipitation and DEAE-Sephacel ion-exchange chromatography.

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Protein Concentration: 1.45 mg/mL

Working Dilution: 1:1600 by indirect ELISA

Western Blot: At a 1:1000 dilution, reacts to 0.3 ug purified ApoAI antigen. Does not cross react with 0.3 ug of Apo AII, B, CI, CII, CIII, or E.

IEP: Single arc vs. purified antigen and whole human plasma.

The ApoAI immunogen used to prepare this antibody was purified in the Lab laboratory. It is a native protein, whole molecule, isolated from human plasma.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

Species Reactivity: Human

Host: Rabbit

Form: Ig Fraction (Standard Antisera) obtained by ammonium sulfate precipitation.

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Protein Concentration: 7.15 mg/mL

Working Dilution: Greater than 1:1000 by indirect ELISA

Western Blot: At a 1:8000 dilution, a strong reaction to 0.15 ug and 0.3 ug antigen.

IEP: Single arc versus antigen.

The Amylase immunogen used to prepare this antibody was purified in the Lab laboratory. It is a native protein, whole molecule, isolated from human pancreas.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

MW: 55,000-65,000 Da
Extinction Coefficient: 0.92

Lyophilized from 30 mM sodium phosphate, pH 6.8

Storage:

Buy Purified Native Angiotensinogen from Human Plasma direct from the manufacturer.
Bulk Qty Available.

Angiotensinogen (AGT) is a glycoprotein found in plasma at a concentration of 2.8-7.1 mg per 100mL in healthy individuals. AGT is produced primarily by the liver but also is synthesized in the brain, large arteries, kidneys and adipose tissue. In plasma it exists as a monomeric form (55-65 kDa) as well as a high molecular mass form (200-550 kDa). In non-pregnant individuals with normal blood pressure, the high molecular mass form accounts for 3% of total circulating AGT. In the first half of pregnancy the total AGT increases by 4-fold and the high molecular mass form increases by 20-fold. Up to 80% of the total AGT in amniotic fluid is the high molecular mass form.

Angiotensinogen is an essential component of the renin-angiotensin system, a potent regulator of blood pressure, body fluid and electrolyte homeostasis. It functions as the substrate for human renin and is the precursor molecule for Angiotensins 1,2,3,4, 1-9, 1-7, 1-5 and 1-4. AGT is also a member of the SERPIN family but it is not known to possess protease-inhibitory activity. Levels in plasma are increased by plasma corticosteroid, estrogen, thyroid hormone and angiotensin-2 levels.

Purity: >=95% by SDS-PAGE

Synonym: Hypertensinogen, Renin substrate

Prepared from plasma shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

MW: 725,000
Extinction Coefficient: 0.81

Lyophilized from 100 mM Na Phosphate, pH 7.2
Storage:

Bulk Qty Available.

Alpha 2 Macroglobulin (A2M) is a plasma protease inhibitor which has been shown to exist in two forms. The Slow Form of A2M (S-A2M) is the form which possesses the ability to bind and inhibit proteases by a “trap” method. The Fast Form of A2M (F-A2M) is generated when S-A2M undergoes a conformational change due to either entrapment of a protease in the A2M bait region, or chemical cleaveage of an internal thiol ester bond located near the bait region. F-A2M does not possess the ability to bind and inhibit protease activity. F-A2M is rapidly taken up by the liver, with a half life of 2-4 minutes.

In vivo, F-A2M typically represents only 0.17–0.7% of the total A2M in blood plasma of adults. The F-A2M plasma concentration is, however, increased in many disease states including pancreatitis, multiple sclerosis and sepsis. F-A2M has also been implicated in the inhibition of amyloid formation associated with Alzheimer’s disease and spongiform encephalopathies.

Purity: >=95% by SDS-PAGE

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.

MW: 725,000
Extinction Coefficient: 0.81

Lyophilized from 100 mM Na Phosphate, pH 7.2
Storage:

Bulk Qty Available.

Alpha 2 Macroglobulin (A2M) is a plasma protease inhibitor which has been shown to exist in two forms. The Slow Form of A2M (S-A2M) is the form which possesses the ability to bind and inhibit proteases by a “trap” method. The Fast Form of A2M (F-A2M) is generated when S-A2M undergoes a conformational change due to either entrapment of a protease in the A2M bait region, or chemical cleaveage of an internal thiol ester bond located near the bait region. F-A2M does not possess the ability to bind and inhibit protease activity. F-A2M is rapidly taken up by the liver, with a half life of 2-4 minutes.

In vivo, F-A2M typically represents only 0.17–0.7% of the total A2M in blood plasma of adults. The F-A2M plasma concentration is, however, increased in many disease states including pancreatitis, multiple sclerosis and sepsis. F-A2M has also been implicated in the inhibition of amyloid formation associated with Alzheimer’s disease and spongiform encephalopathies.

Purity: >=95% by SDS-PAGE

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.