磁微粒化学发光用抗原抗体

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Bulk Qty Available.

MW: 150,000
Extinction Coefficient: 1.36

Lyophilized in 20 mM phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3

IgG is the most abundant immunoglobulin in plasma, found at a concentration of 8 to 18 mg/ml. This immunoglobulin plays a very important role in the defense against infection for newborns because of its transfer through the placenta during pregnancy. It readily diffuses into the extravascular body spaces where it plays a major role in neutralizing bacterial toxins and in enhancing the phagocytosis of microorganisms. IgG binds to bacteria and these complexes adhere to phagocytic cells which have surface receptors specific for IgG.

Storage:

Purity: >=95% by SDS-PAGE

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Product Citations:
Bakovic?, Maja Puc?ic?, et al. "High-Throughput IgG Fc N-Glycosylation Profiling by Mass Spectrometry of Glycopeptides."?Journal of proteome research?12, no. 2 (2013): 821-831

Smith, Loren E., et al. "Helical domains that mediate lipid solubilization and ABCA1-specific cholesterol efflux in apolipoproteins CI and A-II."?Journal of lipid research?54, no. 7 (2013): 1939-1948.

Lee, Kyoung Ho, et al. "Rapid construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering."?Applied microbiology and biotechnology(2013): 1-11.

Dolfi, Douglas V., et al. "Vaccine-Induced Boosting of Influenza Virus-Specific CD4 T Cells in Younger and Aged Humans."?PLOS ONE?8, no. 10 (2013): e77164.

Herati RS, et al. Circulating CXCR5+PD-1+ Response Predicts Influenza Vaccine Antibody Responses in Young Adults but not Elderly Adults. J Immunol. 2014 Oct 1;193(7):3528-37. doi: 10.4049/jimmunol.1302503. Epub 2014 Aug 29.

Fletcher NA, Von Nieda EL, Krebs MD. Cell-interactive alginate-chitosan biopolymer systems with tunable mechanics and antibody release rates.
Carbohydrate Polymers, In press 15 August 2017.

Kammeijer GS, Kohler I, Jansen BC, Hensbergen PJ, Mayboroda OA, Falck D, Wuhrer M. Dopant Enriched Nitrogen Gas Combined with Sheathless Capillary Electrophoresis–Electrospray Ionization-Mass Spectrometry for Improved Sensitivity and Repeatability in Glycopeptide Analysis.
Anal Chem. 2016 Jun 7;88(11):5849-56. doi: 10.1021/acs.analchem.6b00479. Epub 2016 May 12.

Fletcher NA, Babcock LR, Murray EA, Krebs MD. Controlled delivery of antibodies from injectable hydrogels. Mater Sci Eng C Mater Biol Appl. 2016 Feb;59:801-806. doi: 10.1016/j.msec.2015.10.096. Epub 2015 Oct 30.

Fokkink WJR, et al. Comparison of Fc N-Glycosylation of Pharmaceutical Products of Intravenous Immunoglobulin G. PLoS One. 2015 Oct 12;10(10):e0139828. doi: 10.1371/journal.pone.0139828. eCollection 2015.

Tercero N, Kotarek J. Voltammetric Monitoring of Protein Aggregation from Solution Using Tris-(2,2′-Bipyridine) Osmium(II) Chloride Complex as an Electrocatalytic Mediator. Electroanalysis Volume 26, Issue 2 pgs 243-247. 29 JAN 2014 | DOI: 10.1002/elan.20130046.

Ramadass M, Ghebrehiwet B, Smith RJ, Kew RR. Generation of Multiple Fluid-Phase C3b:Plasma Protein Complexes during Complement Activation: Possible Implications in C3 Glomerulopathies. J Immunol. 2014 Feb 1;192(3):1220-30. doi: 10.4049/jimmunol.1302288. Epub 2013 Dec 23.

Swisher JFA, et al. IgG4 can induce an M2-like phenotype in human monocyte-derived macrophages through FcγRI. MAbs. 2014;6(6):1377-84. doi: 10.4161/19420862.2014.975657.

Ref:
Deisenhofer. J. 1981. Biochemistry. 20, 2361; Mehl, V.S. and Penn, G.M. 1986. Manual of Clinical Laboratory Immunology (N.R. Rose, ed.), 126.

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Bulk Qty Available.

MW: 23,000 Da
Extinction Coefficient: 1.45

Frozen in 20 mM phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3

Storage:

Purity: >=95% by SDS-PAGE

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Buy direct from the manufacturer.
Bulk Qty Available.

MW: 200,000
Extinction Coefficient: 1.33

Frozen in 100 mM Tris, pH 7.5, with 200 mM NaCl and 0.05% sodium azide.

IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior.

Storage:

Purity: >=95% by SDS-PAGE

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.

Product Citations:
Janssen, Kris PF, et al. "Multiplexed protein detection using an affinity aptamer amplification assay."?Analytical and bioanalytical chemistry?404, no. 6-7 (2012): 2073-2081.
Schachermeyer, Samantha, et al. "Aptamer-Protein Binding Detected by Asymmetric Flow Field Flow Fractionation."Journal of Chromatography A?(2013).

Abe, Takaaki, et al. "Antibody against immunoglobulin E contained in blood components as causative factor for anaphylactic transfusion reactions."?Transfusion?(2014).

Kashiwakura, Jun-ichi, et al. "Most highly cytokinergic IgEs have polyreactivity to autoantigens."?Allergy, asthma & immunology research?4, no. 6 (2012): 332-340.

Oh SS, et al. Synthetic aptamer-polymer hybrid constructs for programmed drug delivery into specific target cells.
J Am Chem Soc. 2014 Oct 22;136(42):15010-5. doi: 10.1021/ja5079464. Epub 2014 Oct 7.

Cao J, Wang H, Liu Y. Petal-like CdS nanospheres-based electrochemiluminescence aptasensor for detection of IgE with gold nanoparticles amplification. Spectrochim Acta A Mol Biomol Spectrosc. 2015;151:274-9. doi: 10.1016/j.saa.2015.06.104. Epub 2015 Jun 29.

Liu YM, et al. Aptamer-based detection and quantitative analysis of human immunoglobulin E in capillary electrophoresis with chemiluminescence detection. Electrophoresis. 2015 Oct;36(19):2413-8. doi: 10.1002/elps.201500158. Epub 2015 Jul 29.

Poongavanam MV, Kisley L, Kourentzi K, Landes CF, Willson RC.
Ensemble and single-molecule biophysical characterization of D17.4 DNA aptamer-IgE interactions. Biochim Biophys Acta. 2016 Jan;1864(1):154-64. doi: 10.1016/j.bbapap.2015.08.008. Epub 2015 Aug 22.

Garman L, Smith K, Muns EE, Velte CA, et al. Unique Inflammatory Mediators and Specific IgE Levels Distinguish Local from Systemic Reactions after Anthrax Vaccine Adsorbed Vaccination. Clin Vaccine Immunol. 2016 Aug 5;23(8):664-71. doi: 10.1128/CVI.00092-16. Print 2016 Aug.

Ref:
Ishiaka, K. 1985. Methods Enzymol. 116, 76.

Buy direct from the manufacturer.
Bulk Qty Available.

MW: 200,000
Extinction Coefficient: 1.33

Frozen in 100 mM Tris, pH 7.5, with 200 mM NaCl and 0.05% sodium azide.

IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior.

Storage:

Purity: >=95% by SDS-PAGE

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.

Product Citations:
Janssen, Kris PF, et al. "Multiplexed protein detection using an affinity aptamer amplification assay."?Analytical and bioanalytical chemistry?404, no. 6-7 (2012): 2073-2081.
Schachermeyer, Samantha, et al. "Aptamer-Protein Binding Detected by Asymmetric Flow Field Flow Fractionation."Journal of Chromatography A?(2013).

Abe, Takaaki, et al. "Antibody against immunoglobulin E contained in blood components as causative factor for anaphylactic transfusion reactions."?Transfusion?(2014).

Kashiwakura, Jun-ichi, et al. "Most highly cytokinergic IgEs have polyreactivity to autoantigens."?Allergy, asthma & immunology research?4, no. 6 (2012): 332-340.

Oh SS, et al. Synthetic aptamer-polymer hybrid constructs for programmed drug delivery into specific target cells.
J Am Chem Soc. 2014 Oct 22;136(42):15010-5. doi: 10.1021/ja5079464. Epub 2014 Oct 7.

Cao J, Wang H, Liu Y. Petal-like CdS nanospheres-based electrochemiluminescence aptasensor for detection of IgE with gold nanoparticles amplification. Spectrochim Acta A Mol Biomol Spectrosc. 2015;151:274-9. doi: 10.1016/j.saa.2015.06.104. Epub 2015 Jun 29.

Liu YM, et al. Aptamer-based detection and quantitative analysis of human immunoglobulin E in capillary electrophoresis with chemiluminescence detection. Electrophoresis. 2015 Oct;36(19):2413-8. doi: 10.1002/elps.201500158. Epub 2015 Jul 29.

Poongavanam MV, Kisley L, Kourentzi K, Landes CF, Willson RC.
Ensemble and single-molecule biophysical characterization of D17.4 DNA aptamer-IgE interactions. Biochim Biophys Acta. 2016 Jan;1864(1):154-64. doi: 10.1016/j.bbapap.2015.08.008. Epub 2015 Aug 22.

Garman L, Smith K, Muns EE, Velte CA, et al. Unique Inflammatory Mediators and Specific IgE Levels Distinguish Local from Systemic Reactions after Anthrax Vaccine Adsorbed Vaccination. Clin Vaccine Immunol. 2016 Aug 5;23(8):664-71. doi: 10.1128/CVI.00092-16. Print 2016 Aug.

Ref:
Ishiaka, K. 1985. Methods Enzymol. 116, 76.

Purchase IgE direct from the manufacturer.
Bulk Qty Available.

MW: 190,000
Extinction Coefficient: 1.46

Frozen in 15 mM potassium phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3.

IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior.

Storage:

Purity: >=95% by SDS-PAGE

Prepared from human myeloma plasma, Lambda shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Purchase IgE direct from the manufacturer.
Bulk Qty Available.

MW: 190,000
Extinction Coefficient: 1.46

Frozen in 15 mM potassium phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3.

IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior.

Storage:

Purity: >=95% by SDS-PAGE

Prepared from human myeloma plasma, Lambda shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Purchase IgE direct from the manufacturer.

MW: 190,000
Extinction Coefficient: 1.46

Frozen in 15 mM potassium phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3.

IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior.

Storage:

Purity: >=95% by SDS-PAGE

Prepared from human myeloma plasma, Kappa shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Product Citations:
Pérez Ruiz, Elena, Marijn Kemper, Dragana Spasic, Ann Gils, Leo J. van IJzendoorn, Jeroen Lammertyn, and Menno WJ Prins. "Probing the Force-induced Dissociation of Aptamer-Protein Complexes."?Analytical chemistry(2014).

Pérez-Ruiz E, Lammertyn J, Spasic D. Evaluation of different strategies for magnetic particle functionalization with DNA aptamers. N Biotechnol.2016 Dec 25;33(6):755-762. doi: 10.1016/j.nbt.2016.06.1459. Epub 2016 Jun 16.

Arghir J, et al. Improved surface plasmon resonance biosensing using silanized optical fibers. Sensors and Actuators B: Chemical, volume 216 September 2015 pg 518-526.

Ref:
Ishiaka, K. 1985. Methods Enzymol. 116, 76.

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Bulk Qty Available.

MW: 185,000
Extinction Coefficient: 1.7

Frozen in 10 mM Tris-HCl, pH 8.0, with 200 mM NaCl and 0.05% NaN3

IgD was first identified in 1965, and like other immunoglobulins, exists as both secreted and membrane forms. Its level in plasma is low with a mean concentration of 30 ug/ml. IgD is present in large quantities on the surface membrane of a majority of human circulating B lymphocytes. IgD enhances humoral immune responses through its induction of IgD-receptor expression on T lymphocytes.

Storage:

Purity: >=95% by SDS-PAGE.

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Ref:
Coico RF, Siskind GW, Thorbecke GJ 1988. Immunol. Rev. 105:45.

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Bulk Qty Available.

MW: 160,000
Extinction Coefficient: 1.32

Frozen in 10 mM phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3.

In normal adult serum, the approximate percentage composition of IgA with respect to is subclasses is IgA1:90% and IgA2:10%. In secretory IgA, the subclass proportions may approach 50:50. The clinical significance of the subclasses has yet to be determined.

Storage:

Purity: >=95% by SDS-PAGE. No reaction by IEP to IgA1 antiserum.

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Buy direct from the manufacturer.
Bulk Qty Available.

MW: 160,000
Extinction Coefficient: 1.32

Frozen in 10 mM phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3.

In normal adult serum, the approximate percentage composition of IgA with respect to is subclasses is IgA1:90% and IgA2:10%. In secretory IgA, the subclass proportions may approach 50:50. The clinical significance of the subclasses has yet to be determined.

Storage:

Purity: >=95% by SDS-PAGE. No reaction by IEP to IgA1 antiserum.

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Buy direct from the manufacturer.
Bulk Qty Available.

MW: 160,000
Extinction Coefficient: 1.32

Frozen in 10 mM phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3.

In normal adult serum, the approximate percentage composition of IgA with respect to is subclasses is IgA1:90% and IgA2:10%. In secretory IgA, the subclass proportions may approach 50:50. The clinical significance of the subclasses has yet to be determined.

Storage:

Purity: >=95% by SDS-PAGE. No reaction by IEP to IgA2 antiserum.

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.